Anne Daebeler

@anne-daebeler.bsky.social

Microbes, their ecology, physiology, and genomes - especially nitrifiers and CH4 cyclers. Group leader at BC CAS, Czechia. Co-founder of PCI Microbiology. 🦠🐈🌱🏳️‍🌈 https://microbescancycle.wordpress.com

Happy to share our latest article in Environmental Science & Technology! We show that the acidophilic sulfate-reducing bacterium Acididesulfobacillus acetoxydans remains metabolically active under AMD-like acidity through cation transport and membrane remodelling! doi.org/10.1021/acs....

Proton Stress Adaptation in Acidophilic Sulfate-Reducing Bacteria: Insights from Acididesulfobacillus Acetoxydans for Acid Mine Drainage Bioremediation

Acid mine drainage (AMD) waters are a global environmental threat due to their extremely low pH (<3) and high metal loads. Acidophilic sulfate-reducing bacteria (aSRB) can mitigate AMD by reducing sulfate to sulfide, a proton-consuming process that also precipitates metals as metal sulfides. Although sulfate reduction has been observed in AMD waters, most characterized aSRB are only moderately acidophilic. Here, we examined the pH tolerance and proton stress adaptation of the complete organic acid-oxidizing aSRB Acididesulfobacillus acetoxydans. Continuous chemostat cultivations were operated across a pH gradient, reaching steady states from pH 5.0 (optimum) to pH 2.9. In subsequent batch incubations, biomass from a pH 2.9 chemostat remained metabolically active at pH 2.5. Transcriptomic profiles remained remarkably stable across conditions, except for the upregulation of the K+-transporting ATPase (kdpABC) at lower pH, suggesting an increased reliance on the chemiosmotic gradient to impede proton influx. Lipid analysis revealed increased core lipid saturation, midchain methylation, and a shift in priming precursors from leucine to valine at low pH, indicating reduced membrane permeability and more energy-efficient biosynthetic pathways. Together, these adaptations likely reduce proton entry, explaining how aSRB adapt to AMD-like acidity and unlock the pH bottleneck for AMD bioremediation and metal recovery.

doi.org

Reinier Egas@raegas.bsky.social · 10mo ago

New preprint out: Although sulfate reduction at pH < 3 is reported in acid mine/rock drainage environments, isolated aSRB have never matched this limit. Using axenic planktonic chemostats plus DNA/RNA and lipid analyses, we demonstrate aSRB activity and underlying physiology at AMD-like pH.

🔬 New funding opportunity in aquatic microbiology The Symbiosis in Aquatic Systems Initiative (Gordon and Betty Moore Foundation) is inviting proposals for high-risk, high-impact research on microbial symbiosis in marine and freshwater environments. Find out more: buff.ly/vQowRoS

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Do microbes breathe peat? 🦠⚡🌿 New preprint: we sampled Swedish bogs to 7 m and found dominant, uncultured Acidobacteriota & Verrucomicrobiota encode conserved extracellular electron transfer (EET) machinery that was transcriptionally active all the way down.

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How do you make methane from phosphite? Through interspecies H2 transfer! Our paper on lithosyntrophy is finally published - read about how we resolved the metabolic interactions in this phosphite oxidizing, methanogenic enrichment culture! #phosphorus #methanogenesis www.pnas.org/doi/10.1073/...

Lithosyntrophy: Obligate syntrophy in a phosphite-oxidizing, methanogenic culture | PNAS

The anaerobic conversion of organic matter to methane and carbon dioxide typically relies on obligate syntrophic interactions between bacteria and ...

pnas.org

Methane is the second most abundant greenhouse gas with a high heat-trapping capacity but a short lifespan in the atmosphere. So-called “microbial methane munchers” can lower methane emissions from diverse ecosystems, as explored on the new #FEMSmicroBlog. #FascinatingMicrobes

#FEMSmicroBlog: Exploring the metabolic activities of microbial methane munchers

#FEMSmicroBlog: So-called “microbial methane munchers” have the unique abilities to lower methane emissions from various ecosystems.

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Interested in microbial proteins and their diversity? 🖥️🧬🦠 We have recently released the Amino Acid Sequence Toolkit (AASTK). AASTK is designed to work with the GlobDB to create and work with datasets of protein sequences. AASTK currently consists of 4 tools: - CASM - PASR - CUGO - Meta 1/

♨️ Final version now published! 🥳 Explore our provocative new proposal on how to interpret the morphologies of archaean microfossils! 🌋🦠 Thanks to former colleague #DheerajKanaparthi for pulling this all the way through and to @elife.bsky.social for the like! 👍

On the nature of the earliest known lifeforms

Microfossils reported from Archaean BIFs most likely were liposome-like protocells, which had evolved intracellular mechanisms for energy conservation but not for regulating cell morphology and replic...

doi.org

In addition to the 2 postdoc jobs posted yesterday, we’re recruiting 2 PhD students (environmental microbiology/ molecular ecology) to join us from April. The projects will map microbial populations across niches in bioelectrochemical systems relevant to biomethanation & link them to performance.

Two fully funded PhD candidate positions in Electromicrobiology (3 years)

Application deadline: 4 March 2026 at 23:59 hours local Danish time

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