Bergeron lab
@bergeronlab.bsky.social
Structural biology, and beer. Not necessarily in that order.
Happy to share our recent story on the Type VII secretion system. Here were present new insights into the regulation of secretion mediated by the central pore. Thanks to all authors involved in the work. 🙂
bioRxiv Microbiology@biorxiv-microbiol.bsky.social · 5d agoIntrinsic flexibility of Type VII secretion central pore is required for substrate translocation https://www.biorxiv.org/content/10.64898/2026.07.31.742005v1
Interested in 3D-printed models of cryo-EM grids, autogrid and cryo-FIB for visual teaching/decoration. Download our model and print: makerworld.com/en/models/30... Designed in Blender by Khan Bao, an undergrad in the lab.
Cryo-EM Grids for Teaching - Free 3D Print Model - MakerWorld
Download this free 3D print file designed by CiliaBuilder. EM Grid Scaled ModelScaled up and simplified 3D model of EM grids and autogrids for demonstration of preparation process and milling. Content...
makerworld.com
Delighted to host Julien Bergeron @bergeronlab.bsky.social for our next department seminar @ipdbsc.bsky.social onTuesday May 26 👇 @pasteuredu.bsky.social @kingscollegelondon.bsky.social @cnrsbiologie.bsky.social
MX2 Mediates Collapse of the HIV-1 Capsid https://www.biorxiv.org/content/10.64898/2026.05.07.723526v1
Latest pre-print from the lab. Superb work led by former PhD student (now senior scientist at Prosemble) Andy, in close collaboration with the Malim lab.
biorxiv.org
The structure of the Vibrio alginolyticus flagellar filament suggests molecular mechanism for the rotation of sheathed flagella pubmed.ncbi.nlm.nih.gov/42026045/ #cryoEM
Latest paper from the lab - particularly glad that we managed to get this one out, after getting scooped several times over… more spectacular work from PhD students extraordinaire, Kailin and Rosa, in close collaboration with Marc Erhardt’s group.
The structure of the Vibrio alginolyticus flagellar filament suggests molecular mechanism for the rotation of sheathed flagella - Nature Communications
Most bacteria use a flagellum, to swim and disseminate in their environment, and it is essential for virulence. In Vibrio, the flagellum is surrounded by a sheath, a membrane-like shield that protects...
nature.com
Our latest review on the flagellum structure: www.sciencedirect.com/science/chap...
Insights into the structure and diversity of the bacterial flagellum from cryo-EM studies
The bacterial flagellum is a large proteinaceous macromolecular machine that enables motility, host interaction, and virulence across bacterial specie…
sciencedirect.com
Our paper on the in situ structure of the Tad pili machine is now published in mBio journals.asm.org/eprint/D9AT2... #microsky
In situ architecture of the Tad pilus machine in Caulobacter crescentus | mBio
Investigating the Tad pilus nanomachine in a genetically tractable, non-pathogenic organism like Caulobacter crescentus provides a powerful model for elucidating the architecture and functional dynamics of this widespread system. Insights gained from studying the Tad machinery can improve our understanding of related Tad pilus systems in pathogenic bacteria such as Aggregatibacter actinomycetemcomitans, where Tad pili are a key determinant of biofilm formation and chronic infection. Additionally, the remarkable functional diversity of Tad systems, ranging from surface sensing in C. crescentus to bacterial predation in Myxococcus xanthus, highlights their broad biological relevance. By revealing the in situ architecture of the Tad pilus biosynthetic machinery, this study advances our understanding of a major class of bacterial nanomachines and may thus provide structural insights that could inform the development of new therapeutic strategies targeting pilus-mediated virulence.
journals.asm.org
Yves Brun lab@brunlabcaulo.bsky.social · 9mo agoGreat collaboration with @guo-lab.bsky.social to determine an in situ architectural model of the Tad pilus machine. Be on the lookout soon for a preprint on the same topic by Grant Jensen and @viollierpat.bsky.social. #microsky
Molecular basis of high-torque transmission of the Vibrio polar flagellar motor pubmed.ncbi.nlm.nih.gov/41871453/ #cryoEM
I had Claude make this as a mini-game for Relay's disconnect screen (like Chrome's dinosaur), but then pivoted to stand-alone. In this game, you have to find Euler angles matching the target projection, i.e. cosplay RELION. It can be pretty hard. Works on mobile too! warpem.github.io/euler-hunt/
Euler Hunt
Can you find the Euler angles of a cryo-EM projection?
warpem.github.io
Our latest pre-print! More superb work led by duo extraordinaire Kailin and Rosa (The order of names is wrong, I should not be listed first…)
The structure of the Vibrio alginolyticus flagellar filament suggests molecular mechanism for the rotation of sheathed flagella
In several pathogenic bacteria, including Vibriospecies, the filament of the bacterial flagellum is encased by a membranous sheath, an extension of the bacterial outer membrane. It has been proposed t...
researchsquare.com
I probably shouldn’t comment on how amazing and transformative I think this work is, since I’m examining the thesis of its first author in a few weeks. So I’m not going to.
Basil Greber@bjgreber.bsky.social · 10mo agoNow out in Science! Cyclin-dependent kinases (CDKs) are key regulators of the cell cycle. In @vcushing.bsky.social's magnum opus, we use #cryoEM to figure out how the CDK-activating kinase recognises CDKs to fully activate them - a key step in cell cycle control. www.science.org/doi/10.1126/...
@kingscollegelondon.bsky.social spin-out company Prosemble, co-founded by @bergeronlab.bsky.social has been awarded @innovateuk.bsky.social funding as part of the Biomedical Catalyst to transform cancer care with cutting-edge nanoparticle and AI technologies. Read more: www.kcl.ac.uk/news/kings-s...
King's spin-out company awarded Innovate UK Biomedical Catalyst funding | King's College London
King's College London spin-out company Prosemble, co-founded by Dr Julien Bergeron, has been awarded Innovate UK funding as part of the Biomedical Catalyst to transform cancer care with cutting-edge n...
kcl.ac.uk
That is very cool.
Tanmay Bharat@tbharat-lab.bsky.social · 11mo agoSub-cellular chemical mapping in bacteria using correlated cryogenic electron and mass spectrometry imaging Congrats Hannah Ochner and authors on this important paper! Strong collaboration with @kiranrpatil.bsky.social www.biorxiv.org/cgi/content/... @mrclmb.bsky.social @wellcometrust.bsky.social
The structure of the Tad pilus alignment complex reveals a periplasmic conduit for pilus extension pubmed.ncbi.nlm.nih.gov/40730569/ #cryoEM
The peer-reviewed version of Sash and Iryna’s paper on the Tad pilus is out:
The structure of the Tad pilus alignment complex reveals a periplasmic conduit for pilus extension - Nature Communications
The Tad pilus is involved in the formation of antibiotic-resistant biofilms in many bacteria, including the human pathogen Pseudomonas aeruginosa. The authors report the structure of the Tad pilus pro...
nature.com
Hmmmm… there’s a, let’s call it “interesting” new sculpture at the entrance of the @crick.ac.uk . I’m curious to hear what this is meant to illustrate.
Superb work from our lab neighbours!
Atherton Lab KCL@athertonlabkcl.bsky.social · last yr.Excited to see our @natcomms.nature.com paper out! #Cryo-EM reveals a #microtubule stimulated ADP-release transition state of #kinesin 1. The auto-inhibitory tail has no affect on motor domain conformations. Focussed subunit processing helps! #cryoEM #microtubules www.nature.com/articles/s41...
In a new study, @bergeronlab.bsky.social have used the world’s most advanced electron microscope to reveal the complete architecture of the bacterial flagellum - a molecular machine essential for bacteria to cause infection, that could also be a target for antibiotics ⬇️ www.kcl.ac.uk/news/scienti...
Scientists target 'molecular machine' in the war against antimicrobial resistance
Scientists have studied a new target for antibiotics in the greatest detail yet – in the fight against antibiotic resistance.
kcl.ac.uk
#NewResearch 🚨Out now! Structural analyses of the extracellular flagellum shows mechanism for flagellin incorporation #MicroSky 🦠 www.nature.com/articles/s41...
The structure of the complete extracellular bacterial flagellum reveals the mechanism of flagellin incorporation - Nature Microbiology
Cryo-electron microscopy analysis of the extracellular flagellum structure, including the hook–filament junction and filament cap complex, reveals mechanisms of flagellin incorporation into the growin...
nature.com
A lovely write-up from Kings about our latest paper, and its role in combating antibiotic resistance:
Scientists target 'molecular machine' in the war against antimicrobial resistance
Scientists have studied a new target for antibiotics in the greatest detail yet – in the fight against antibiotic resistance.
kcl.ac.uk
The peer-reviewed version of our article on the flagellum filament assembly is out! A masterpiece from duo extraordinaire Kailin and Rosa, and an incredibly productive collaboration with Marc Erhardt and Morgan Beeby.
The structure of the complete extracellular bacterial flagellum reveals the mechanism of flagellin incorporation - Nature Microbiology
Cryo-electron microscopy analysis of the extracellular flagellum structure, including the hook–filament junction and filament cap complex, reveals mechanisms of flagellin incorporation into the growin...
nature.com
When they go low, you go high, said a very wise woman Just goes to show how pathetic Europe is exactly 💪🏻🧪 (Well done European Commission and ERC)
Craig Nicholson@clanicholson.bsky.social · last yr.ERC doubling funding to lure researchers to the EU www.researchprofessionalnews.com/rr-news-euro...
A family of bacterial actin homologs forms a three-stranded tubular structure pubmed.ncbi.nlm.nih.gov/40073056/ #cryoEM
Latest paper from the lab: the Cryo-EM structure of BeeR! (Yes we did name the protein. No it’s not a coincidence)
A family of bacterial actin homologs forms a three-stranded tubular structure | PNAS
The cytoskeleton is crucial for cell organization and movement. In Eukaryotes, it largely consists of the protein actin, that forms a double-strand...
pnas.org
Curvature generation and engineering principles from Shewanella oneidensis multi-flagellin flagellum www.biorxiv.org/content/10.1101/2025.02.07.637127v1 #cryoEM
