Daniel Krüger
@danielkrueger.bsky.social
Researcher in the Netherlands. Postdoc with Hans Clevers. Organoid models. Sozi aus Deutschland.
Short write-up of our journey. Searching for T cell transcription factors, Marc v/d Wetering stumbled across Wnt signaling. Vladja Korinek linked Wnt to colon cancer and crypt biology. Nick Barker discovered the gut stem cell. Toshi Sato invented organoids authors.elsevier.com/a/1n3No6tu0C...
Intestinal villus cells extrude living, following a calcium pulse. Unlike from extruding apoptotic cells, Ca2+ messaging stops at the first neighbors! #FluorescenceFriday Part of our TrepatLab preprint: www.biorxiv.org/content/10.1... Video by @ronjahoutekamer.bsky.social @gloerichlab.bsky.social
Great to see our conclusions align with the beautiful work from @marija-matejcic.bsky.social in the Trepat lab 3D force mapping, Ca²⁺-triggered myosin-IIA meshwork dissolution, and neighbour lamellipodia generating traction. Great synergy, highlighting the role of tension in extrusion regulation.
Levayer Lab@levayerr.bsky.social · 11mo agoThanks for highlighting this! My background in fly morphogenesis shaped how I think about tissue forces, their interplay with signaling, and experimental design. An experience I’d wholeheartedly recommend — Drosophila remains a rich source of inspiration, continuing to reveal fundamental concepts.
Levayer Lab@levayerr.bsky.social · 11mo agoI may have a biased interpretation here, but I suspect the background of @danielkrueger.bsky.social in fly morphogenesis brought a lot here (congrat for the impressive work !) , a good example of virtuous background mixing and work built on important conceptual/technical premise from model organism
This is very complementary with the recent preprint of @xaviertrepat.bsky.social and Maria Matejcic using 2D organoids and providing 3D mechanical characterisation of the process
Important story dissecting the mechanism of gut cell extrusion using organoids. Local heterogeneity of tension promotes live extrusion and basal relaxation is sufficient to kick the cell out. Nice combination of optogenetics and live imaging to demonstrate this www.science.org/doi/abs/10.1...
👉 Check out our key findings in this detailed thread by Hans Clevers.
Hans Clevers@hansclevers.bsky.social · 11mo ago1/11 In @science.org: A new perspective on how our intestines renew. Cells are not “pushed out” by crowding or die from apoptosis. Instead, cells play a mechanical tug-of-war, where weaker cells extrude, reframing gut renewal as force-regulated. www.science.org/doi/10.1126/...
Out in @science.org! We found that the intestine doesn’t just push out cells when crowded. Instead, cells are in a mechanical tug-of-war, and weaker ones are extruded. A new view of how our gut keeps its balance and stays healthy. www.science.org/doi/10.1126/...
Epithelial tension controls intestinal cell extrusion
Cell extrusion is essential for homeostatic self-renewal of the intestinal epithelium. Extrusion is thought to be triggered by crowding-induced compression of cells at the intestinal villus tip. In th...
science.org
📣 It is my pleasure to announce our recent review with Margherita on the current approaches, challenges, and perspectives in studying human maternal-fetal interface using in vitro models www.cell.com/cell-stem-ce... 🚀✨️
Modeling the human maternal-fetal interface
This review explores the advances in stem cell and organoid models of the endometrium, placenta, and embryo, outlining challenges in integrating them to capture spatiotemporal dynamics and functional ...
cell.com
FMI science@fmiscience.bsky.social · 11mo agoIn a Review just published in @cp-cellstemcell.bsky.social, @vladbndk.bsky.social and Margherita Turco explore how stem cell and organoid models can be used and combined to better study the maternal–fetal interface. A 🧵
Jeey! @nfdi4bioimage.bsky.social published their #RDM-related postcards FAIRly (made by Henning Falk, licensed CC-BY 4.0) 🔬💾😍 github.com/NFDI4BIOIMAG...
First time that my cartoons are printed on postcards! Have a look 👇
NFDI4BIOIMAGE@nfdi4bioimage.bsky.social · 2y agoOh yes, we did! Here are our new postcards - educational outreach material that pinpoints challenges in bioimaging #RDM complexity. Turn them around to find out about solutions and support! And all illustrations are free for reuse (CC-BY). doi.org/10.5281/zeno... Many thanks to Henning Falk!
Exciting study by Amanda Andersson Rolf et al: In vitro expansion of a human fetal #pancreas #stemcell that generates all three pancreatic cell lineages”. www.cell.com/cell/fulltex... New #organoid protocol allows the seemingly indefinite expansion of human fetal pancreatic Lgr5+ stem cell 1/
Long-term in vitro expansion of a human fetal pancreas stem cell that generates all three pancreatic cell lineages
Identification of an LGR5 as a marker for a tripotent stem/progenitor cell of the human fetal pancreas. Organoids derived from single LGR5+ cells are capable of long-term expansion in vitro and genera...
cell.com
"The state of German-Polish relations is a European tragedy in the same way that the Warschauer Kniefall was a moment of European reconciliation." Brilliant analysis by @JeremyCliffe and also an important read before the Polish elections this weekend. There is so much at stake.
Europe’s east-west divide is widening
Relations between Germany and Poland define the continent. But, ahead of the latter’s election this weekend, they are in crisis and getting worse.
newstatesman.com
Anhören! Wie sich große branchenfremde Investoren (ALDI, Immobilien etc) riesengroße Ackerflächen 🚜 in Ostdeutschland kaufen. Am Staat vorbei, ohne Steuern zu zahlen, mit dem Segen des Bauernverbandes und zum Schaden der kleinen Landwirtschaftsbetriebe. 👇
Warum Investoren immer mehr Ackerland kaufen
Überregionale Investoren kaufen immer mehr Ackerland in Sachsen-Anhalt auf, die Preise sind stark gestiegen. Seit der Energiewende gibt es noch mehr Konkurrenz.
share.deutschlandradio.de
Jugendarbeitslosigkeit in der EU, August 2023: 🇪🇸26.8% 🇪🇪23.5% 🇬🇷22.5% 🇷🇴22.3% 🇮🇹22.0% 🇸🇪21.1% 🇵🇹20.3% 🇭🇷20.0% 🇸🇰19.8% 🇱🇺19.2% 🇨🇾16.3% 🇫🇮16.1% 🇫🇷16.0% 🇧🇪14.2% 🇪🇺14.0% 🇭🇺12.8% 🇧🇬11.8% 🇩🇰11.6% 🇦🇹11.4% 🇮🇪11.2% 🇵🇱11.1% 🇸🇮10.4% 🇱🇻9.0% 🇲🇹9.0% 🇳🇱8.7% 🇱🇹8.6% 🇨🇿8.5% 🇩🇪5.7% via: ec.europa.eu/eurostat/doc...
