Robertas Ursache

@robertasu.bsky.social

Group leader at CRAG in Barcelona. Interested in cell wall remodeling, cutin and suberin dynamics, optimizing gene editing and histological techniques

📆 Deadline approaching today! 🌾Are you interested in how plant cells perceive and respond to mechanical signals?🧪 Apply for this postdoctoral scholarship and join @stephanevrg.bsky.social's group. 👉 See the original post for details and application.

Umeå Plant Science Centre@umeaplantsciencecentre.se · 3mo ago

🧪🌾VACANCY: Postdoctoral scholarship in Plant Cell Adhesion and Mechanosensing🔬 Interested in how plant cells perceive and respond to mechanical signals? Join @stephanevrg.bsky.social’s group and work on fundamental questions in plant cell biology. Apply by June 22👇: www.umu.se/en/work-with...

Not sure which scRNA-seq platform to use for 🪴 plant samples? How well do doublet detection algorithms really work in 🌱? How can I optimise sample prep ? Find out in our benchmark study! Led by @carogro.bsky.social and @thomaseekhout.bsky.social. Open access at EMBO Journal: doi.org/10.1038/s443...

Benchmarking plant single cell RNA-sequencing sample processing strategies - The EMBO Journal

The isolation of single plant cells from complex tissues is prone to selective enrichment and sampling biases, which complicates accurate profiling of the large diversity in cell types. Optimizing methodologies for cell enrichment and single-cell transcriptomics is therefore critical for single-cell studies addressing plant cell heterogeneity. Here, we systematically compared protoplast enrichment technologies (including conventional and image-based flow cytometry, as well as magnetic cell sorting) and single-cell RNA sequencing (scRNA-seq) platforms (10X Genomics Chromium, BD Rhapsody) using Arabidopsis roots. Image-based flow cytometry offered increased precision due to customizable gating strategies, while magnetic sorting provided faster processing and enhanced representation of cell size heterogeneity. Both scRNA-seq platforms captured root cell heterogeneity and yielded reproducible gene expression profiles, but showed platform-associated differences in cell type composition. Notably, single-nucleotide polymorphism analysis of a mixed ecotype sample revealed that, among cells identified as doublets by computational algorithms, two-thirds were likely to have been misclassified. These insights identify key biases in plant cell purification and scRNA-seq workflows and provide practical guidance for improving data quality across plant species.

doi.org

A synthetic fluorescent nanobody platform for multicolor imaging - thanks to the de Oliveira, Nimmerjahn, and Verkhusha Labs and the whole team! 🧬Find the plasmids here: www.addgene.org/browse/artic...

Addgene: Synthetic multicolor antigen-stabilizable nanobody platform for intersectional labeling and functional imaging.

addgene.org

Sofia de Oliveira, Ph.D.@sdeoliveira.bsky.social · 3mo ago

🧪Thrilled to see this work out in @Nature Methods from Verkhusha Lab with Natalia Barykina as 1st author @einsteinmededu.bsky.social VIS–Fbs is a multicolor fluorescent nanobody platform for imaging, and #Zebrafish helped extend it into an in vivo vertebrate system. Verry happy to be part of it 🤗

Plasmidsaurus RNA-Seq is now processed in Cologne for our European customers! Ship at ambient temperature with SEQguard Dino Preserve 🦖 • 3-day turnaround • $50/sample (academia), $80 (industry) • Gene expression analysis • Up to ~10M unique transcript 3’ end reads per sample • Interactive results

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