Inducible cysteine synthesis by CysK2 provides copper resistance in Mycobacterium tuberculosis | mBio

Copper is a proposed component of the host immune response against Mycobacterium tuberculosis; thus, an understanding of how this pathogen mitigates copper stress may yield new targets for therapeutic...

journals.asm.org

A coupled TOM–TIM22 supercomplex in human mitochondrial protein import

Nature Structural & Molecular Biology, Published online: 31 July 2026; doi:10.1038/s41594-026-01852-1TOM and TIM22 complexes facilitate the transport of carrier protein substrates into mitochondria. New work shows that, in human mitochondria, these two protein complexes form transient supercomplexes to facilitate protein biogenesis.

dlvr.it

An adaptor for feedback regulation of heme biosynthesis by a mitochondrial protease

Heme biosynthesis is tightly coordinated to support essential functions without accumulating toxic porphyrins and depleting cellular iron. Heme induces degradation of the heme biosynthetic enzyme, 5-a...

science.org

UTR-ly unexpected: RNA chaperones tame intrinsically disordered proteins

How do cells ensure that complex, multidomain proteins fold correctly? Luo et al. reveal a self-contained solution. The 3′UTR of an mRNA co-translationally chaperones the protein it encodes, preventin...

cell.com

A tail of two ligases - Nature Chemical Biology

Targeted protein degradation eliminates disease-driving proteins by co-opting the ubiquitin–proteasome system. Now, a single degradation tail enables a molecular glue to hijack two E3 ubiquitin ligase...

nature.com

GID4-Recruiting PROTACs for BRD4 Degradation Overcome Resistance Driven by CRBN and VHL Deficiency

Despite the identification of over 600 E3 ligases, current proteolysis-targeting chimeras (PROTACs) predominantly rely on CRBN and VHL. Recently, GID4, a substrate receptor of the CTLH E3 ligase compl...

pubs.acs.org

Assembly of the catalytic module and the rotor of human ATP synthase - The EMBO Journal

Human ATP synthase is a molecular rotary machine bound in inner mitochondrial membranes, built from twenty-eight subunits of seventeen kinds, two encoded in mitochondrial DNA, the remainder in nuclear...

link.springer.com

Stress-induced ribosome degradation in Bacillus subtilis is mediated by the RNase Y-specificity complex - Nature Communications

Transcriptional regulation is a central mechanism underlying bacterial adaptation to stress. Here, the authors used a genetic fitness screen to show that the Y-complex in Bacillus subtilis contributes...

nature.com

Activation mechanism and structural assembly of the Mycobacterium tuberculosis ClpP1P2 protease and its associated ATPases

Weinhäupl et al. determine the cryo-EM structure of the ClpC1P1P2 complex from Mycobacterium tuberculosis, revealing an asymmetric architecture and selective activator binding. They show that molecula...

cell.com

The N-degron pathway regulates glucose and insulin homeostasis through the lysosomal degradation of RXRA/RXRα via SQSTM1/p62

Central to the pathogenesis of type 2 diabetes (T2D) is the failure in insulin secretion from pancreatic β-cells associated with insulin resistance. The nuclear receptor RXRA/RXRα (retinoid X recep...

tandfonline.com

FEBS Press

AAA+ unfoldases are essential molecular motors that power protein degradation and disaggregation. This review integrates recent cryo-electron microscopy (cryo-EM) structures and single-molecule bioph...

febs.onlinelibrary.wiley.com

From Doubt to Discovery: A 15-Year Journey into Proteostasis, Pathogens, and Persistence

More than 15 years ago, during my PhD, we stumbled upon something unexpected. In a yeast two-hybrid screen, bacterial effector proteins from Xanthomonas and Pseudomonas interacted with a subunit of…

theustunlab.com

A dynamic gateway: Uncovering the expanded human TOM complex interactome and its regulatory complexity

Human TOM complex was revealed as a dynamic hub, with FKBP8 as a human-specific interactor shaping biogenesis.

science.org

biorxiv.org

PNAS

Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans...

pnas.org

A C-degron regulates Chk1 kinase by allowing stability of inactive Chk1 and by making it short- lived upon activation | PNAS

The Arg/N-degron pathway of Saccharomyces cerevisiae is mediated by two interacting E3 ubiquitin ligases, Ubr1 and Ufd4. We show here that the mito...

pnas.org

Structural dynamics of the midnolin-proteasome during ubiquitin-independent substrate turnover - Nature Communications

Proteasomes typically degrades ubiquitin-tagged proteins, but midnolin enables a distinct ubiquitin-independent pathway. Here, the authors present cryo-EM structures of the midnolin-proteasome complex...

nature.com

NorQD AAA+ complex drives metal insertion by a twisting mechanism - Nature Communications

AAA+ -ATPases drive diverse molecular remodeling processes in cells. Here, the authors characterize the interaction between the NorQ ATPase and its VWA domain partner NorD, and propose a twisting mech...

nature.com

A molecular switch in NAC prevents mitochondrial protein mistargeting by SRP - Nature Communications

How ribosome-bound NAC distinguishes mitochondrial precursors from ER clients has remained unclear. Here, authors reveal a molecular switch in NAC that limits SRP access to nascent mitochondrial precu...

nature.com

Structure-function analysis of the bacterial ClpE/ClpP AAA+ protease

General and regulatory proteolysis in bacteria is executed by a set of ATP-dependent proteases composed of hexameric ring-forming AAA+ proteins and associated peptidase barrels (e.g. ClpP). These AAA+...

jbc.org

biorxiv.org

Research Group Leader Tenure Track - Structural Studies - LMB 2775 - Medical Research Council

Location: Cambridge. Vacancy: Research Group Leader Tenure Track - Structural Studies - LMB 2775. Closing Date: 16/03/2026, 23:55

mrc.tal.net

A dynamic displacement mechanism drives protein import into mitochondria

Most mitochondrial proteins are produced in the cytosol and imported through the translocase of the outer mitochondrial membrane (TOM) to reach their final destination. Although this protein entry gat...

biorxiv.org

The ribosome synchronizes folding and assembly to promote oligomeric protein biogenesis

Large oligomeric proteins constitute a major fraction of proteomes, but are difficult to refold in vitro, raising the question of how cells direct their biogenesis. Roeselová and Shivakumaraswamy et a...

cell.com

Single-molecule dynamics of the TRiC chaperonin system in vivo - Nature

Single-particle tracking experiments in intact cells reveal dynamic co- and post-translational interactions of the TRiC–PFD chaperonin complex with client proteins during in vivo protein folding.

nature.com

Molecular mechanisms of mitochondrial AAA+ proteases

Mitochondrial AAA+ proteases, LONP1, ClpXP, YME1L (i-AAA), and the m-AAA complex, maintain protein quality and shape organelle function. Growing interest in these enzymes stems from their association ...

jbc.org