This study shows that third-harmonic generation (THG) is enhanced in a wavelength-dependent manner by biological pigments. This mechanism can be used to detect absorbing structures in THG microscopy. @laboptbio.bsky.social @cnrs.fr @inserm.fr @ipparis.bsky.social 🔗 https://aippub.org/4dNQCv4

Resonant third harmonic generation in biological pigments

Third harmonic generation (THG) microscopy provides label-free structural contrast of biological tissues. While the THG process can be enhanced by electronic tr

pubs.aip.org

Preprint is out! Our BakingTray software turns a 2-photon microscope into a serial-section tomography system for automated whole-organ imaging. ~€35k to convert an existing scope. 11 systems worldwide, >7,000 samples at SWC alone #neuroskyence #microscopy www.biorxiv.org/content/10.6...

@stelladees.bsky.social et al.'s work on THG in pigments is featured in #Scilight @aip.bsky.social 🔹 doi.org/10.1063/10.0...

A label-free imaging technique can distinguish biological pigments

The method identifies tiny interfaces within a material by monitoring variations in refractive index and absorption, making it sensitive to pigments.

doi.org

Emmanuel Beaurepaire@ebeaurepaire.bsky.social · 2mo ago

Resonant third harmonic generation in biological pigments Dees et al, APL Photonics 2026 doi.org/10.1063/5.03... A new article from @laboptbio.bsky.social microscopy unit @cnrs.fr @inserm.fr @xpolytechnique.bsky.social @ipparis.bsky.social (1/5)

A nice study by @laboptbio.bsky.social colleagues V.Abdelsayed, P.Mahou,G.Latour,MC.Schanne-Klein and collaborators in J.Ramella-Roman's group at FIU. They use polarized SHG to analyze the reorganization of cervix collagen matric during gestation in mice. Congrats Vaky & co! doi.org/10.1063/5.03...

Advanced optical polarimetric microscopy reveals spatio-temporal dynamics of cervix remodeling during gestation

Polarimetric microscopy is a powerful tool for assessing fiber orientation and reorganization during various biological processes. Collagen fiber remodeling in

doi.org

Resonant third harmonic generation in biological pigments Dees et al, APL Photonics 2026 doi.org/10.1063/5.03... A new article from @laboptbio.bsky.social microscopy unit @cnrs.fr @inserm.fr @xpolytechnique.bsky.social @ipparis.bsky.social (1/5)

Resonant third harmonic generation in biological pigments

Third harmonic generation (THG) microscopy provides label-free structural contrast of biological tissues. While the THG process can be enhanced by electronic tr

doi.org

A new paper from the LOB microscopy unit on high-speed multiphoton light-sheet imaging has been published in Optica. We achieved kilohertz frame rates by enhancing the signal-to-photodamage ratio during live imaging. doi.org/10.1364/OPTI...

🚀 KiloHz two photon live imaging #MultiphotonMicroscopy @laboptbio.bsky.social Two-photon light-sheet live imaging at kilohertz frame rate using birefringence-based pulse splitting #LightSheetMicroscopy #Bioimaging #Photonics #Microscopy #Zebrafish Article is available here: doi.org/10.1364/OPTI...

Caroline Boudoux, Professor at @polymtl.bsky.social and entrepreneur will give the next LOB seminar: "It Goes without Saying: Taking the Guesswork of Your PhD in Engineering" See you on April 17th at 11AM in Amphi Becquerel. lob.ip-paris.fr/en/events/se...

Seminar of Caroline Boudoux - Professor at Polytechnique Montréa et an entrepreneure (Castor Optics)

11:00am, Ecole Polytechnique, Amphi Becquerel

lob.ip-paris.fr

An INSERM CHAIR (tenure track position) has just been opened in the Laboratoire d'Optique et Biosciences (LOB) (INSERM U1182) in @xpolytechnique.bsky.social / @ipparis.bsky.social @inserm.fr Deadline to apply : September 2. pro.inserm.fr/wp-content/u...

Our paper “Label-free nonlinear microscopy probes cellular metabolism and myelin dynamics in live tissue” is published in @commsbio.nature.com, thanks to an exciting collaboration between @laboptbio.bsky.social and @institutducerveau.bsky.social www.nature.com/articles/s42...

Label-free nonlinear microscopy probes cellular metabolism and myelin dynamics in live tissue - Communications Biology

Multimodal label-free nonlinear microscopy and advanced image analysis map single-cell metabolism and myelin dynamics in live cerebellar tissue during demyelination, supporting research in neuroinflam...

nature.com

(2025/10/08) Congratulations to @chiarastringari.bsky.social on the defense of her Habilitation à Diriger les Recherches (HDR) "Label-free multimodal non-linear optical microscopy for functional imaging of living tissues" !

Hey, it's out! doi.org/10.1016/j.ce... Aligning Big Brains and Atlases, ABBA in short, is published in Cell Reports @cp-cellreports.bsky.social . @biancasilvalab.bsky.social's lab added an analysis pipeline on top of it (BraiAn) and did all the relevant biological work. What is it ? Short thread:

Multiscale characterization of myelin distribution with polarized THG microscopy Morizet et al, Optica 2025 doi.org/10.1364/OPTI... @laboptbio.bsky.social @institutducerveau.bsky.social @cnrs.fr ‪‪ @ip-paris.bsky.social @inserm.fr (1/8)

Congratulations to @caporalclement.bsky.social on his brilliant defence of his PhD thesis, entitled 'Quantitative Analysis of Large Volume Imaging in Developmental Neurobiology' !

Two postdoc positions in the lab within the ERC Synergy project "Chronology" in collaboration with S. Ostojic, M. Jazayeri and V. van Wassenhove @virginievanw.bsky.social . Two focuses: sound sequences representations across the brain & novel all-optical technology. Contact: brice.bathellier@cnrs.fr

🆕 We're hiring! We're looking for postdoc 🎓 candidates to work at the intersaction of bioimage analysis 🖥️🔬, tissue biomechanics 🧫, and neuroscience 🧠. 👉 Application deadline: 2025/06/02 👉 Duration: July 2025 - December 2026 (for now) Come work with us! 🤗 dipc.ehu.eus/en/dipc/join...

Joint Postdoctoral Position in Biophysics – Bioimage Analysis / AI / Neuroscience

dipc.ehu.eus

Congratulations to Stella Dees for defending her thesis on "Multicontrast 3P-microscopy for biological tissue imaging"

Congratulation to Giulia Galante for her thesis defense about the "Characterization and physico-chemical monitoring of collagen degradation in parchments by polarization-resolved multiphoton microscopy!

See you on Friday February 21st for the @eurobioimaging.bsky.social Virtual Pub on "Fluorescence Lifetime Microscopy (FLIM) for label-free metabolic imaging of living tissues"! #FLIM #labelfree #microscopy #metabolism You can register online here! www.eurobioimaging.eu/events/flim-...

Do you need to analyze your FLIM data with open source software? The #Virtual #I2K2024 workshop on "Interactive #phasor analysis of #FLIM data using FLUTE" given by Bahar Asadipour is online. www.youtube.com/watch?v=q7W1... #FLUTE can analyze data of most commercial cards github.com/LaboratoryOp...

Interactive phasor analysis of FLIM data using FLUTE

YouTube video by I2K Conference

youtube.com

Un poste d’ingénieur en analyse d’images est ouvert en mobilité pour rejoindre @laboptbio.bsky.social à @polytechniqueparis.bsky.social (Palaiseau). Poste IR ouvert aux IE de la fonction publique et IR @cnrs.bsky.social. Deadline: 20/02. Lien (concours 508): profilsdemplois.cnrs.fr/ords/afipprd...

An image analysis engineer position is open for mobility to join @laboptbio.bsky.social at @polytechniqueparis.bsky.social ky.social in Palaiseau. IR position open to civil service IEs and CNRS IRs. Deadline: 20/02. Link (position #508): profilsdemplois.cnrs.fr/ords/afipprd...