Rob Campbell

@raacampbell.com

Advanced Microscopy Facility Manager Sainsbury Wellcome Centre, UCL http://swcmicroscopy.com

A new design framework brings two-photon miniscopes to 1g! Our lab's MINI2P-II (1.1g) overcomes the widely accepted assumption that high optical performance and ultralow weight cannot coexist. A climbing mouse can carry two, a singing songbird one 🧵 www.biorxiv.org/content/10.6...

Multi-region two-photon calcium imaging in freely moving animals

Complex behaviors emerge from the coordinated activity of neural circuits distributed across widely separated brain regions. Existing optical approaches for monitoring neural activity lack the combine...

biorxiv.org

Remember how power bricks used to be bigger, heavier, hotter, and less powerful than power modern power supplies? The SNES power supply can provide 8.5W and this much smaller GaN USB-PD can supply 65W, at greater efficiency. Well, the same thing is probably going to happen to grid transformers. 🧵

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"A water industry source said these plans were not published to avoid alarming the public. The government said the plans were not published for security reasons" Following Starmer's line, Burnham & Co. refuse to inform the public about what's coming Insane www.theguardian.com/environment/...

UK government ‘irresponsible’ for not publishing emergency drought plans

Campaigners demand greater transparency into water companies’ plans not made public due to ‘security reasons’

theguardian.com

Excited to share our work now in Nature Methods! We wrote this manuscript to help others better analyze and interpret calcium imaging data. As a central aspect, we show why nonlinear behavior of calcium indicators matters in practice. Very happy about this work!

Nature Methods@natmethods.nature.com · 3mo ago

An Analysis from @ptrrupprecht.bsky.social et al investigates the behavior of GCaMP8 variants in spike inference using different algorithms. www.nature.com/articles/s41...

Methods, trade-offs and opportunities in high-speed optical microscopy for neural voltage imaging ->Nature | More info at BigEarthData.ai

Methods, trade-offs and opportunities in high-speed optical microscopy for neural voltage imaging

Adam, Y. et al. Voltage imaging and optogenetics reveal behaviour-dependent changes in hippocampal dynamics. Nature 569, 413–417 (2019). Villette, V. et al. Ultrafast two-photon imaging of a high-gain voltage indicator in awake behaving mice. Cell 179, 1590–1608 (2019). Fan, L. Z. et al. All-optical physiology resolves a synaptic basis for behavioral timescale plasticity. Cell 186, 543–559 (2023). Wenzel, M., Hamm, J. P., Peterka, D. S. & Yuste, R. Reliable and elastic propagation of cortical seizures in vivo. Cell Rep. 19, 2681–2693 (2017). Sato, T. K., Nauhaus, I. & Carandini, M. Traveling waves in visual cortex. Neuron 75, 218–229 (2012). Zhao, H. T. et al. Neurovascular dynamics of repeated cortical spreading depolarizations after acute brain injury. Cell Rep. 37, 109794 (2021). Zhang, Y. et al. Fast and sensitive GCaMP calcium indicators for imaging neural populations. Nature 615, 884–891 (2023). Manley, J. et al. Simultaneous, cortex-wide dynamics of up to 1 million neurons reveal unbounded scaling of dimensionality with neuron number. Neuron 112, 1694–1709 (2024). Yang, Y., Li, B. & Li, Y. Genetically encoded sensors for the in vivo detection of neurochemical dynamics. Annu. Rev. Anal. Chem. 17, 367–392 (2024). Wei, Z. et al. A comparison of neuronal population dynamics measured with...

nature.com

At Cosyne, Chris Olah from Anthropic told us that neuroscientists had “3-4 years left to make a contribution” Why was not clear. Perhaps he was hinting at the below graph accompanying today’s Claude Science launch. Apparently we’ll all be obsolete soon.

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Our new preprint 'NucleiSky enables cross-scale multimodal registration of microscopy data using nuclei constellations' is out! 🎉Check out @guijacquemet.bsky.social post first to learn more🤩 In this 🧵 I’ll share how we used NucleiSky in smart microscopy and how you can install and try it yourself 👇

Guillaume Jacquemet@guijacquemet.bsky.social · 4mo ago

Delighted to share our latest preprint "NucleiSky enables cross-scale multimodal registration of microscopy data using nuclei constellations" www.biorxiv.org/content/10.6... Code and App: github.com/CellMigratio...

Since I did do my PhD in cyanobacteria growth and treatment optimization modeling AND work in the water treatment industry, I’m gonna share my theory as to what’s happening here and how to fix it. People are focusing on the painted bottom of the pool increasing residual heat, and while that 1/19

derek guy@dieworkwear.bsky.social · 5mo ago

RIP Mark Rothko. You would have loved how the hydrogen peroxide bleach is only turning the Reflecting Pool blue around the perimeter