With Cryo-ET/EM, the macromolecules we study (and biological questions we ask) are SNR-limited. Here, we share Nilas, a low-voltage milling method named after thin sea ice. Nilas produces thin (sub 50 nm in places!), minimally damaged lamellae. biorxiv.org/content/10.64898/2026.07.21.739890v1

Optimized cryo-FIB milling strategy to generate thin, minimally damaged biological lamellae

Focused ion beam (FIB)-milling has been adapted to thin frozen cells for visualization of macromolecular structures in situ with cryogenic electron microscopy. However, only a few large and abundant c...

biorxiv.org

In a series of 3 papers and preprints, we’re thrilled to share with you the working laser phase plate. In collaboration with research led by Holger Müller at UC Berkeley, this is a huge innovation in imaging to make small and faint objects inside cells visible. bit.ly/4vK9LVn

XLPP in practice www.biorxiv.org/content/10.6...

We're happy to announce our new preprint! 🐸 easymode: general pretrained networks for cellular cryo-ET. Segment ~20 cellular features – ribosomes, microtubules, mitochondria, nuclei & more – with zero model training. 🔗 doi.org/10.64898/202... 🧵👇

New preprint🧊🔬Introducing LowDoseWizard—a guided SerialEM workflow for rapid, standardised cryo-TEM setup! Setting up low-dose can be slow & daunting, especially for new users. LowDoseWizard automates optics, camera, and alignment steps from minimal input — full setup in <15 min. #cryoEM #SerialEM

cryoEM papers@cryoempapers.bsky.social · 3mo ago

LowDoseWizard - rapid and standardised setup of low-dose cryo-TEM imaging in SerialEM www.biorxiv.org/content/10.64898/2026.05.05.722937v1 #cryoEM

We're super excited to share MissAlignment: a new ML-based approach to reference-free tilt series alignment, spearheaded by @martenchaillet.bsky.social. We think it's going to make your cryo-ET life a lot better. Preprint: www.biorxiv.org/content/10.6... Code: github.com/warpem/miss-... 🧶 Thread:

If you wanna try our new Autogrid High-Pressure Freezing Planchettes ❄️ - you can use the provided drawings & CAD files to produce them in-house 🛠️ - you can now also contact www.wohlwend-hpf.ch to order yours with a shiny gold-coating ✨ The preprint for instructions ➡️ www.biorxiv.org/content/10.6...

Preprint 🧵! I’m really excited to introduce BIGSMALL, an interleaved multi-magnification cryo-ET scheme bridging molecular and cellular scales in a single acquisition! BIGSMALL = 𝐁road 𝐈nformation 𝐆athering 𝐒trategy by 𝐌ultiscale 𝐀cquisition of lame𝐋𝐋a www.biorxiv.org/content/10.6... (1/6)