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Inga Songailiene

@ingasongailiene.bsky.social

🇱🇹 Biochemist, PhD. CRISPR and TA systems, anti-phage response, nucleases, Cryo-EM. MSCA postdoc at N.Taylor lab in CPH 🇩🇰

56 Posts207 Follower318 Folgt
Anna Lopatina@annalopatina.bsky.social · 3w ago

Heads-up! I’ll be starting my research group at MedUni Vienna in January 2027, with postdoc and PhD positions available soon. Looking for people interested in bacteria–phage interactions, bacterial defense systems, and phage anti-defense strategies. Please send your CV: anna.lopatina@univie.ac.at

Alexander Harms@aharms485.bsky.social · last mo.

The final version of our manuscript on TnSeq in bacteriophages (“HIDEN-SEQ”) is out today – if you have an interesting phage phenotype and want to know the underlying genetic basis, this one is for you! 1/4

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Inga Songailiene@ingasongailiene.bsky.social · 2mo ago

Structure of P2 phage is out on bioRxiv! 🔍📖 This phage is like a spoiled kid - hard to propagate, purify, get infection, etc! - but instead of doing it, you can now just read a paper by @vkleinsousa.bsky.social 🪄👏

Victor Klein-Sousa@vkleinsousa.bsky.social · 2mo ago

I'm happy to share one of my favorite projects from the past 4 years. 'Piercing mechanism in the minimal contractile tail of bacteriophage P2'. In this preprint, we applied #cryo-EM, #cryo-ET, TEM, and #computationalbiology to study the #phage P2 virion structure and its contraction mechanism.

Khaled_Selim Lab@selimlab.bsky.social · 2mo ago

A new preprint from the lab! 📄 We solved a long-standing question: how mechanistically the PTM AMPylation of glutamine synthetase controls the activity of one of the most important enzymes on Earth. 🌎 w/ beautiful cryoEM structures @hhu.de & @sfb1381.bsky.social www.biorxiv.org/content/10.6...

Metabolic regulation and structural mechanism of glutamine synthetase AMPylation

In bacteria, glutamine synthetase (GS) is the main ammonium assimilation enzyme. Its activity is tightly regulated according to cellular energy status and carbon/nitrogen balance through reversible AM...

biorxiv.org

Inga Songailiene@ingasongailiene.bsky.social · 2mo ago

Happy to share our review article on CRISPR-Cas spacer aquistion "Building CRISPR immunity: evolution and mechanisms of spacer acquisition". 🎏 It was a great pleasure to contribute to it! www.sciencedirect.com/science/arti...

Building CRISPR immunity: evolution and mechanisms of spacer acquisition

CRISPR–Cas systems in prokaryotes serve as adaptive immune systems that neutralize phage infections through RNA-guided nucleases. Immunization is achi…

sciencedirect.com

Tominaga K. (tomiken)@pacyc184.bsky.social · 2mo ago

A tRNA-derived second messenger mediates antiviral defense | bioRxiv

A tRNA-derived second messenger mediates antiviral defense

CRISPR-Cas systems are RNA-guided nucleases that enable prokaryotic immunity; however, some loci encode additional associated genes that cooperate with CRISPR effectors to perform diverse biological functions. Here, we uncover a CRISPR-associated kinase (CASK) system that links the recognition of target RNA to protein phosphorylation. We show that the kinase Csx33 phosphorylates Csx34 following activation of Cas13, enabling Csx34 to bind DNA in a sequence-specific manner. Together, Csx33 and Csx34 function as a transcriptional activation module that upregulates cas13 and associated genes, revealing a positive autoregulatory circuit that potentiates the immune response upon detection of foreign RNA. At the molecular level, Csx33 is activated by CCA trinucleotide RNA generated by Cas13-mediated cleavage of tRNA 3′ tails, uncovering a novel linear second messenger in bacterial immunity and a previously unrecognized signaling role of collateral RNA fragments. Together, these findings establish CASK systems as a new platform for RNA sensing and for engineering programmable phosphorylation-based signaling systems. ### Competing Interest Statement The authors have declared no competing interest. National Heart, Lung, and Blood Institute Division of Intramural Research, https://ror.org/023ny1p48, DP2-HL185100

biorxiv.org

Typas Lab@typaslab.bsky.social · 2mo ago

Excited to share our paper with @savitski-lab.bsky.social, led by @tarabartolec.bsky.social, Karin Mitosch & Clément Potel, out now in @nature.com. We show that T7 phage broadly disables bacterial defenses using a unique ‘loose cannon’ protein kinase. www.nature.com/articles/s41...

Pervasive phosphorylation by phage T7 kinase disarms bacterial defences - Nature

The T7 bacteriophage deactivates bacterial defence systems through T7K phosphorylation of nearly all host and phage proteins during infection.

nature.com

Typas Lab@typaslab.bsky.social · 2y ago

Excited to share our preprint with @savitski_lab led by @tarabartolec.bsky.social, @KMitosch & Clément Potel! We uncovered a mechanism by which the T7 phage broadly counteracts DNA-targeting bacterial defenses by deploying a loose cannon kinase in its genome @embl.org www.biorxiv.org/content/10.1...

Inga Songailiene@ingasongailiene.bsky.social · 2mo ago

Phosphorylation by T7 kinase inactivates Eco9 retron and DarTG TA systems, as well as targets many other DNA/RNA-binding proteins! ✨️🪄 Very grateful for the authors that they tested our plasmids: MNT-HEPN and ApeA systems 🙏

Pervasive phosphorylation by phage T7 kinase disarms bacterial defences - Nature

The T7 bacteriophage deactivates bacterial defence systems through T7K phosphorylation of nearly all host and phage proteins during infection.

nature.com

Jens Hör@jenshoer.bsky.social · 3mo ago

Do you need to analyze bacterial microscopy data and are looking for an all-in-one tool for Windows or macOS? Check out BactoMate by Lasse Hallenga and Philipp Popp! I'm happy that we were able to contribute to this great work. www.biorxiv.org/content/10.6...

BactoMate: an integrated platform for reproducible bacterial microscopy analysis

Quantitative microscopy of microorganisms increasingly produces large, multidimensional datasets, yet their analysis often depends on fragmented workflows spanning file conversion, segmentation, quali...

biorxiv.org

Inga Songailiene@ingasongailiene.bsky.social · 3mo ago

With such a diversity of mechanisms, anti-phage defence should soon become a standard chapter in immunology textbooks, covering general principles across all three domains of life. 🤹

Kranzusch Lab@kranzuschlab.bsky.social · 3mo ago

The shared principles of human and bacterial antiviral immunity An honor to highlight remarkable discoveries from many labs over the past 12 years that unite previously disparate fields of how animal cells and prokaryotes defend against viruses. www.nature.com/articles/s41... rdcu.be/fzwyB

Landon Getz@landongetz.bsky.social · 3mo ago

DNA glycosylases are repair enzymes, essential to all cellular life. They find chemically damaged bases and remove them so DNA can be repaired. Today in Nature Microbiology, we show bacteria have repeatedly repurposed them into antiviral immune systems 🧵 www.nature.com/articles/s41...

Antiviral defence is a conserved function of diverse bacterial DNA glycosylases - Nature Microbiology

DNA glycosylases, typically used for DNA repair, can also protect bacteria against phages that would otherwise evade host immunity by incorporating modified bases into their genomes.

nature.com