Colleagues and I address a recent phylogenetic & evolutionary hypothesis on mosquito evolution (from Pierce et al. 2025), namely that the two subfamilies of mosquitoes were not reciprocally monophyletic, and that Culex and Anopheles were sister to one another. We do not agree.
The Culicinae are Monophyletic and Ancient: A response to Pierce et al. 2025 https://www.biorxiv.org/content/10.64898/2026.05.04.720205v1