Krista Freeman
@kfreeman.bsky.social
Big phan of physics & phages | cryo-EM, phage engineering, a little immunology | e-bike enthusiast CWRU Physics Assistant Professor Cleveland kid.
(1/6) Thrilled to share this story! In our preprint from my PhD in the @kranzuschlab.bsky.social, with help from the Hatfull lab and @soreklab.bsky.social, we discover RyDEP, a phage-encoded RyR-domain glycosidase that allows phages to evade Thoeris defense. Highlights below! doi.org/10.64898/202...
Well, I just was denied the COVID vaccine in Cleveland, Ohio. I can’t begin to express the rage I feel at being barred from protecting my vulnerable loved ones. My “senators” just got voicemails - I will keep calling until access to these safe and effective vaccines is restored. My body, my choice.
Join me in donating to ABRCMS. For many of us early in our careers (and for many of us now), this is THE conference. Let’s make sure future generations get the same experience. I was told if each poster judge donated $25, their goal would be quickly met, but I think we can do better! 🙏🏾💪🏾
Katie Wilkinson@drkwilkinson.bsky.social · 12mo agoCalling all @apsphysiology.bsky.social members: let’s help raise some money to support ABRCMS student presentation awards! Goal is $1200/discipline to support 12 awards by Sept 30th! 🧪 abrcms.org/donate/stude...
Together with leading science organizations, ASM filed an amicus brief calling for immediate restoration of NIH grants supporting early-career scientists. Read the press release: asm.org/press-releas...
Four Leading Science Organizations File Amicus Brief Supporting Funding Restoration
ASM joined leading life science organizations in an amicus brief filed in support of a suit calling for immediate funding restoration.
asm.org
Now online! Structure and infection dynamics of mycobacteriophage Bxb1
Structure and infection dynamics of mycobacteriophage Bxb1
Cryo-EM and cryo-ET reveal the structural details and conformational dynamics of mycobacteriophage Bxb1 as it infects its Mycobacterium smegmatis host.
dlvr.it
Krista Freeman@kfreeman.bsky.social · last yr.Hurray, it is finally out! Meet bacteriophage Bxb1 - the subject of my first full-phage cryo-EM study. My structures are beautifully complemented by the Park Lab’s cryo-ET analysis, shedding light on mycobacteriophage structural changes during infection. www.sciencedirect.com/science/arti...
Some eye-catching #TeamTomo work here. Congrats to @kfreeman.bsky.social and all the authors! 🧪🧶🧬🔬
ᐯIᑕTOᖇ ᑎIᘔET, ᗰᗪ@nizet.bsky.social · last yr.Krista Freeman et al 𝘊𝘌𝘓𝘓 stunning atomic-level imaging (Cryo-EM, cryo-ET) reveals how 𝐛𝐚𝐜𝐭𝐞𝐫𝐢𝐨𝐩𝐡𝐚𝐠𝐞 Bxb1 reshapes its tail tip to breach the mycobacterial cell wall and deliver DNA to the cytoplasm can inform precise targeted phage therapies for TB and NTM infections www.cell.com/cell/fulltex...
Krista Freeman et al 𝘊𝘌𝘓𝘓 stunning atomic-level imaging (Cryo-EM, cryo-ET) reveals how 𝐛𝐚𝐜𝐭𝐞𝐫𝐢𝐨𝐩𝐡𝐚𝐠𝐞 Bxb1 reshapes its tail tip to breach the mycobacterial cell wall and deliver DNA to the cytoplasm can inform precise targeted phage therapies for TB and NTM infections www.cell.com/cell/fulltex...
Host invasion by a mycobacteriophage—visualized in action! 🦠✨ Thrilled to have contributed to this exciting work! Great collaboration with the amazing Krista @kfreeman.bsky.social & Graham Hatfull lab. Huge thanks to the Park lab for this fantastic opportunity 🙌 #CryoEM #CryoET #phage #mycobacteria
Krista Freeman@kfreeman.bsky.social · last yr.Hurray, it is finally out! Meet bacteriophage Bxb1 - the subject of my first full-phage cryo-EM study. My structures are beautifully complemented by the Park Lab’s cryo-ET analysis, shedding light on mycobacteriophage structural changes during infection. www.sciencedirect.com/science/arti...
Do you love phages? Other viruses? Cool science done by cool people? Check out this paper by my former bay mate @kfreeman.bsky.social and colleagues that sheds light on the cool molecular machines that are phage tails.
Krista Freeman@kfreeman.bsky.social · last yr.Hurray, it is finally out! Meet bacteriophage Bxb1 - the subject of my first full-phage cryo-EM study. My structures are beautifully complemented by the Park Lab’s cryo-ET analysis, shedding light on mycobacteriophage structural changes during infection. www.sciencedirect.com/science/arti...
Hurray, it is finally out! Meet bacteriophage Bxb1 - the subject of my first full-phage cryo-EM study. My structures are beautifully complemented by the Park Lab’s cryo-ET analysis, shedding light on mycobacteriophage structural changes during infection. www.sciencedirect.com/science/arti...
Structure and infection dynamics of mycobacteriophage Bxb1
Mycobacteriophage Bxb1 is a well-characterized virus of Mycobacterium smegmatis with double-stranded DNA and a long, flexible tail. Mycobacteriophages…
sciencedirect.com
Excited about progress we are making to build a system for preclinical interrogation of #phages for #Tb #TBSKY virologyj.biomedcentral.com/articles/10....
Evaluation of host immune responses to Mycobacteriophage Fionnbharth by route of delivery - Virology Journal
For much of the last decade, tuberculosis (TB) was the leading cause of mortality due to an infectious pathogen (Mycobacterium tuberculosis, M.tb). Approximately 1.3 million deaths in 2023 worldwide w...
virologyj.biomedcentral.com
Use of epigenetically modified bacteriophage and dual beta-lactams to treat a Mycobacterium abscessus sternal wound infection. DOI: 10.1038/s41467-024-54666-4 https://pubmed.ncbi.nlm.nih.gov/39609405/
Use of epigenetically modified bacteriophage and dual beta-lactams to treat a Mycobacterium abscessus sternal wound infection - PubMed
Nontuberculous mycobacterium (NTM) infections are challenging to manage and are frequently non-responsive to aggressive but poorly-tolerated antibiotic therapies. Immunosuppressed lung transplant patients are susceptible to NTM infections and poor patient outcomes are common. Bacteriophages present …
pubmed.ncbi.nlm.nih.gov
Come join us at CWRU Physics! We're hiring in Condensed Matter Experiment at assistant professor level. 🧪⚛️ case.edu/academic-car...
Assistant Professor (tenure-track) in Experimental Condensed Matter Physics - Department of Physics | Academic Careers | Case Western Reserve University
The Department of Physics at Case Western Reserve University invites applications for a tenure-track faculty position at the rank of Assistant Profes...
case.edu
Thanks for making this starter pack, Joscelyn! Awesome to see so many MOSAIC scholars assembling here in the bluer skies. So happy to connect!
Joscelyn Mejías, PhD@jcmejias.bsky.social · 2y agoI started a list of MOSAIC scholars to help find and support each other. I'm sure I've missed many people, lmk if you want to be added! go.bsky.app/9Ui711k
LOVING the NIH BioArt effort! BUT… where are the phages?? Hoping to see some in the official release! Anyone know if there’s a mechanism to request additions? #PhageSky
I’m pleased to share that I’ll be joining Case Western Reserve University as an Assistant Professor of Physics, starting July 2025! Looking forward to returning to my Physics roots while continuing to investigate the wonderful world of phages. :)
Big congrats to Graham Hatfull on being elected a member of the National Academy of Sciences! I couldn’t ask for a better mentor. So glad to have had the opportunity to learn from him the last 7 (!) years!!
Home from my first ever ABRCMS and feeling, in a word, uplifted! I hope to come back year after year to cultivate friendships, learn from mentors, and connect with the amazing next generation of diverse scientists. Huge thanks to the ASM MOSAIC program for the warm welcome :)
PhamClust: a phage genome clustering tool using proteomic equivalence @mSystemsJ Methods and Protocols from Graham Hatfull journals.asm.org/doi/10.1128/...
Just test driving this new platform… apparently if I add a test tube my post will go to the ScienceSky feed? 🧪 And I suppose threads can be done with a string of comments on a post? So far so good… the waters do seem calmer here.
Krista Freeman@kfreeman.bsky.social · 3y agoHi, my name is Krista… a recent transplant from Science Twitter. I’m a postdoc studying bacteriophages - viruses that infect bacteria. When I’m not reconstructing cool phage structures or working on my faculty applications, I love traveling and exploring the great outdoors.
Hi, my name is Krista… a recent transplant from Science Twitter. I’m a postdoc studying bacteriophages - viruses that infect bacteria. When I’m not reconstructing cool phage structures or working on my faculty applications, I love traveling and exploring the great outdoors.