Our latest paper is out! 🎉 We show that the HSV-1 origin binding protein UL9 tethers dsDNA thereby forming intramolecular DNA loops and intermolecular DNA tethers. 🤝 www.nature.com/articles/s41...

KymoSketch started out as a GUI for our kymotracker script, but after I added DeepDiffusion to it I got suggestions to add more features and it’s since become a more fleshed out analysis platform (1/6)

Korak Ray@korakray.bsky.social · last mo.

🚨 Preprint alert 🚨 Excited to share our latest bioRxiv preprint introducing DeepDiffusion, a tool for analysing facilitated 1D diffusion! 🔗 Preprint: www.biorxiv.org/content/10.6... (1/3)

🚨 Preprint alert 🚨 Excited to share our latest bioRxiv preprint introducing DeepDiffusion, a tool for analysing facilitated 1D diffusion! 🔗 Preprint: www.biorxiv.org/content/10.6... (1/3)

DeepDiffusion: a Physics-Informed Neural Network for Heterogeneous Facilitated 1D Diffusion

Single-molecule fluorescence microscopy combined with optical tweezers has enabled the direct observation of diffusing proteins on tethered DNA. These and complementary techniques reveal facilitated o...

biorxiv.org

The first paper from my postdoc, looking at how tension activates DNA cleavage by the endonuclease ANKLE1, is out in @narjournal.bsky.social. ANKLE1 processes ultrafine bridges, persisting linkages between chromosomes that can lead to catastrophic DNA ruptures. (1/3) doi.org/10.1093/nar/...

We're looking for a new Roving Research @mrc-lms.bsky.social A unique post for those who love research, learning new techniques & working across different teams. Must be a great communicator & well organised! Apply by 21 June: fa-evzn-saasfaukgovprod1.fa.ocs.oraclecloud.com/hcmUI/Candid...

Investigator Scientist Roving Researcher

Investigator Scientist (Roving Researcher) (LMS 2369) Salary £42,159 – 44,801 plus London Allowance (£5,560) per annum | 2 years fixed term | Full time | Closing Date: 21 June

fa-evzn-saasfaukgovprod1.fa.ocs.oraclecloud.com

1/11 In our story out today in @nature.com , we showcase the molecular mechanisms that enable Cas9 to bind and cleave negatively supercoiled ((-)SC) off-targets! 🧬🧵 🔗 www.nature.com/articles/s41... #CRISPR #Cas9 #DNATopology #AFM #CryoEM #SingleMolecule #Biophysics

Structural basis of supercoiling-induced CRISPR–Cas9 off-target activity - Nature

Cas9 structures explain topology sensing and off-target activation.

nature.com

🔬 Calling all early-career researchers (ECRs) for the GRS Single Molecule Approaches to Biology! 🌎 Location: Les Diablerets, Switzerland 📆 Save the date: June 27-28, 2026 🔗 More information: www.grc.org/single-molec... #biology #biophysics #single-molecule #microscopy #ECR

Slightly overdue article highlight! In PNAS, we use smFRET to reveal how polypeptide release factor 2 (RF2) undergoes a cascade of structural changes to catalyze polypeptide release during translation termination and ArfA-mediated ribosome rescue. 🧵⬇️ doi.org/10.1073/pnas...

A bit of good news! Part of my PhD work in collaboration with @ssjang.bsky.social and David Lynall is out in @narjournal.bsky.social! We uncover a surprising mechanism by which an ultrastable RNA stem-loop tunes its flexibility, enabling it to both fold rapidly and resist unfolding.

Ruben Gonzalez@rubenlgonzalez.bsky.social · last yr.

New from our lab in @narjournal.bsky.social: We dissect the folding dynamics of a fundamental element of RNA secondary structure—a stem-loop—at single-molecule and microsecond resolution. doi.org/10.1093/nar/... 1/5

Are you at #bps2025 and want to hear about: HSV-1 DNA replication, DNA looping, #smFRET, TIRF, optical tweezers or cryoEM? Come and talk to me at my poster B175 on Wed, 10.30am!

Exciting new research from the Cellular Stress and Single Molecule Imaging groups has created a new technique for transfecting cells that could pave the way for new gene therapies! We'll be recapping some of the highlights from the article in this thread. 🧵1/6

Precise intracellular uptake and endosomal release of diverse functional mRNA payloads via glutathione-responsive nanogels

We present a novel, highly customizable glutathione-responsive nanogel (NG) platform for efficient mRNA delivery with precise mRNA payload release con…

sciencedirect.com

tMAVEN, our open-source comprehensive platform for single-molecule data analysis, is finally here! Check out our article describing the platform and how we use it to investigate the use of kinetic models to extract mechanistic info from data, out in Biophys J. www.sciencedirect.com/science/arti...

Increasing the accuracy of single-molecule data analysis using tMAVEN

Time-dependent single-molecule experiments contain rich kinetic information about the functional dynamics of biomolecules. A key step in extracting th…

sciencedirect.com

Spooky season reminder that, at its core, The Monster Mash is a cautionary tale about working in the lab late one night and, ultimately, the dangers of poor work/life balance under late capitalism.

🚨 Preprint news!! 🚨 The collaboration with @ckinzthompson on a novel definition of resolution and how it can be used to calculate the local atomic resolution and map-to-model correspondence in cryoEM structures is finally on bioRxiv!!!! www.biorxiv.org/content/10.1... 🧵(1/6)

Scale-dependent hierarchical resolution: applications to atomic resolution and model validation in c...

bioRxiv - the preprint server for biology, operated by Cold Spring Harbor Laboratory, a research and educational institution

biorxiv.org

BREAKING NEWS: Three scientists have won the 2023 #NobelPrize in Chemistry for their research on the discovery and synthesis of quantum dots. Moungi G. Bawendi, Louis E. Brus and Alexei I. Ekimov share prize for research that added color to nanotechnology: cen.acs.org/people/nobel...