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KPL ApS

@kplaps.bsky.social

At the Cutting-Edge of Protein Innovation. Learn more at https://kplbio.com/ .

17 Posts95 Follower293 Folgt
KPL ApS@kplaps.bsky.social · 6mo ago

🧪 Just launched: Trypsin/Lys-C Premium Mix 🚀 We found that with the right formulation, you can add Lys-C and Trypsin together instead of sequentially. So we made it 💡 ✅ One step ✅ Lowest missed cleavages in its class ✅ Fully recombinant 🪧Available now 🔗 kplbio.com/trypsinlysc/

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KPL ApS@kplaps.bsky.social · 7mo ago

Does enzyme quality matter in #proteomics? New study in JPR w/ @jesperolsenlab.bsky.social shows: LysC-Trypsin reduces missed cleavages from 30% → 15% KPL's A. lyticus LysC achieves >90% efficiency in systematic comparison. Congrats to the authors! pubs.acs.org/doi/10.1021/... #TeamMassSpec

Comparative Analysis of Lysine-Specific Peptidases for Optimizing Proteomics Workflows

This study presents a comparative analysis of three LysC endopeptidase homologues from Achromobacter lyticus (A. lyticus),Pseudomonas aeruginosa and Lysobacter enzymogenes for mass spectrometry-based proteomics. Utilizing a protein aggregation capture workflow with HeLa cell lysates, we assessed the enzymes’ cleavage specificity, digestion efficiency, and performance across various experimental conditions. Results showed that while all three LysC homologues exhibited high cleavage specificity at lysine residues, A. lyticus LysC outperformed the two others with superior peptide identification, digestion efficiency, and protein coverage, especially at shorter digestion times. Our experiments using a combination ofA. lyticusLysC and trypsin demonstrated the importance of employing LysC for significantly minimizing missed cleavage rates in tryptic digests, especially with regard to lysine-containing peptides. This study underscores A. lyticus LysC’s potential as an optimal choice for enhancing mass spectrometry-based proteomics.

pubs.acs.org

KPL ApS@kplaps.bsky.social · 7mo ago

Happy Holidays from KPL! 🎄 Our first full year: 2 product launches, lots of foundation building, and countless hours pushing #proteomics forward. Our commitment: high quality, high performance, competitive prices. No compromises. 2026 is looking exciting - stay tuned! ✨

Shabaz Lab@shabazlab.bsky.social · last yr.

A basic solution to a very common problem. collab with @kusterlab.bsky.social and @bendavisgroup.bsky.social www.mcponline.org/article/S153...

Addressing NHS chemistry: Efficient quenching of excess TMT reagent and reversing TMT-over labelling in proteomic samples by methylamine

N-Hydroxysuccinimide (NHS) ester chemistry is used extensively across proteomics sample preparation. One of its increasingly prevalent applications is in isobaric reagent-based quantitation such as iT...

mcponline.org

Shabaz Lab@shabazlab.bsky.social · last yr.

Shout out to the organisers @jesperolsenlab.bsky.social, (Tina Ludwig) @baybioms.bsky.social kathrynslilley.bsky.social, Caroline May and Britta Eggers and the speakers @bonalditiziana.bsky.social, @coscialab.bsky.social, @kusterlab.bsky.social, @anamdv.bsky.social, @jsandow.bsky.social

Shabaz Lab@shabazlab.bsky.social · last yr.

The Brixen Proteomics Summer School speaker list has been finalised!. Lots of amazing science to be discussed! Big thanks to @eupaproteomics.bsky.social @msaid-de.bsky.social @thermofishersci.bsky.social @biognosys.bsky.social @ionopticks.bsky.social @evosep.bsky.social for support

KPL ApS@kplaps.bsky.social · 2y ago

🚀Introducing Arg-C Zero (KPL0146)🧬 Next-gen proteomic enzyme with close to 0% missed cleavages at Arginine residues! 🔬100% Arginine cleavage + ultimate specificity = next-level protein analysis magic ✨ 🧬 Details: kplbio.com/product/arg-c/ #Proteomics #TeamMassSpec #proteins

Arg-C Zero - High-Fidelity Protease for Mass Spectrometry | KPL Bio

Discover Arg-C Zero [KPL0146], a high-fidelity arginyl endopeptidase delivering 0% missed cleavages. Ideal for proteomics, Arg-C Zero ensures complete digestion, high purity, and compatibility with ma...

kplbio.com

Devin Schweppe@dschweppe.bsky.social · 2y ago

That’s a wrap, we’ve uploaded our version of record for the large scale PISA work led be Jon & co. to @elife.bsky.social : elifesciences.org/articles/95595 Thanks to the reviewers for helping us improve the manuscript, enjoyed the process of developing this resource.

Large-scale characterization of drug mechanism of action using proteome-wide thermal shift assays

An approachable framework for the scalable implementation of proteome-wide thermal shift assays to assess drug mechanisms of action.

elifesciences.org