I’m excited to share this article from my PhD, exploring the role and prevalence of peptide deformylases! It has been a truly enriching and fascinating scientific journey. Thank you, @amazeld.bsky.social, for welcoming me into your lab and giving me the scientific freedom to follow my curiosity!

Didier Mazel@amazeld.bsky.social · 10mo ago

We've come full circle! I began my postdoctoral career by identifying the peptidyl deformylase gene. Today, we show that half of bacterial species harbor multiple PDF genes (up to 7, for always a single Met-tRNA transformylase), and while the role of these PDFs ... academic.oup.com/mbe/article/...

Thrilled to share my first PhD paper, out of the Mazel lab! @amazeld.bsky.social We discovered that accessory peptide deformylases (PDFs) are widespread in bacteria and may provide resistance to natural inhibitors, posing a challenge to future antibiotic strategies doi.org/10.1101/2025...

Unraveling the prevalence and multifaceted roles of accessory peptide deformylases in bacterial adaptation and resistance

Peptide deformylases (PDFs) are enzymes that are essential for bacterial viability and attractive targets for antibiotic development. Yet, despite their conserved function, many bacteria encode multip...

doi.org

First preprint from my PhD is now online! We show that deep learning can recognise modes of action of antibiotics directly from brightfield images of bacteria. 🦠

bioRxiv Microbiology@biorxiv-microbiol.bsky.social · 2y ago

Deep learning recognises antibiotic modes of action from brightfield images https://www.biorxiv.org/content/10.1101/2025.03.30.645928v1

Out today @ Science: TIR signaling activates caspase-like immunity in bacteria We report a new immune signaling molecule: N7-cADPR. Produced by phage-induced TIRs and activates a defensive bacterial caspase Congrats Francois Rousset, Ilya Osterman and coauthors! www.science.org/doi/10.1126/...

New preprint from our Lesterlin Lab! Yannick Baffert and @nfrk92.bsky.social, in collaboration with @macerwan.bsky.social and @val-meve.bsky.social, shed light on the molecular mechanisms driving pOXA-48 plasmid maintenance and transfer. We reveal novel TA system, regulators, and T4SS components!

Genetic Determinants of pOXA-48 Plasmid Maintenance and Propagation in Escherichia coli

Conjugative plasmids are the main drivers of antibiotic resistance dissemination contributing to the emergence and extensive spread of multidrug resistance clinical bacterial pathogens. pOXA-48 plasmi...

biorxiv.org

🚨 New preprint! We know that plasmids are associated with very variable fitness costs in their different bacterial hosts. But, what is the contribution of each of the plasmid-genes in these host-specific effects? Study led by @jorgesastred.bsky.social, @sanmillan.bsky.social and myself! 1/14

Dissecting pOXA-48 fitness effects in clinical enterobacteria using plasmid-wide CRISPRi screens

Conjugative plasmids are the main vehicle for the spread of antimicrobial resistance (AMR) genes in clinical bacteria. AMR plasmids allow bacteria to survive antibiotic treatments, but they also produ...

biorxiv.org

Welcome to the official Bluesky page of the Institut Pasteur! 🔬 Explore with us the frontiers of biomedical research 🧪 Follow us to uncover groundbreaking discoveries 💡 Share knowledge for global health 🌍 Join a community passionate about progress and open science.

www.nature.com/articles/s41... New paper out!!

A plasmid-chromosome crosstalk in multidrug resistant enterobacteria - Nature Communications

The authors describe a new crosstalk between a globally disseminated carbapenem resistance plasmid and clinical enterobacteria clones. This crosstalk provides a fitness advantage to the plasmid-carryi...

nature.com

👋 Hi antiphage defense community, we are soon releasing an update of DefenseFinder. We are doing our best to include all the great discoveries from the community, but with so much going on we might miss things. Please answer wt preprints/papers with new systems or mail/git them to us. 🙏

🚨New review🚨 Evolution and ecology of anti-defence systems in phages and plasmids Link: www.cell.com/current-biol...

1/ 🔬 Our work, "Dynamic transitions of initiator binding coordinate the replication of the two chromosomes in Vibrio cholerae", is now published in Nature Communications. Here's a thread on how we think Chr1 and Chr2 replication is coordinated in Vibrio. 🧵 Link : www.nature.com/articles/s41...

Our paper on 🦠queuosine 34 modification of tRNA tyrosine and antibiotic response is finally out in its final form in @eLife ... and quite different from the preprint version. Many thanks to reviewers, editors and kudos to all authors🙏 elifesciences.org/articles/96317

Aminoglycoside tolerance in Vibrio cholerae engages translational reprogramming associated with queuosine tRNA modification

tRNA Q34 modification impacts tyrosine codon decoding and leads to proteome reprogramming in response to antibiotic stress in Vibrio cholerae.

elifesciences.org