And here it is: the final protocols for your future favorite 16S rRNA gene primer pair for anplicon sequencing studies -> V4EXT. P.S. Mighty are the small ones - metric💩tons of Patescibacteria everywhere. Except in 💩, that is. academic.oup.com/ismecommun/a...

Revised 16S rRNA V4 hypervariable region targeting primers enhance detection of Patescibacteria and other lineages across diverse environments

Abstract. Primer bias in 16S rRNA gene amplicon sequencing can distort microbial diversity estimates by underrepresenting key taxa. We introduce a modified

academic.oup.com

I did not do justice to the great work of @bcoltman.bsky.social on MetaCoOc in this thread. A manuscript describing the tool fully, and a stable release of the code, are in preparation. In the meantime, the code can be found here: github.com/bcoltman/met...

In addition, the environmental metadata is included in the amino acid sequence toolkit (AASTK) SQL database, and can be directly accessed using the Meta command. Thus, any dataset of GlobDB derived AA sequences can be annotated with their primary environments. More on this here: globdb.org/aastk

We now provide environmental metadata for the GlobDB genomes! 🖥️🧬🦠 More info here: globdb.org/news 🧵

Our new preprint introduces #kineochelins, a new group of #siderophores produced by the Antarctic soil actinomycete 🦠🔬🧫 #Actinokineospora sp. UV203 Kudos to the amazing Stanislava Králová & a fantastic team of collaborators 🤩 #microbiomesky www.biorxiv.org/content/10.6...

Phage–host interactions are a key driver of microbial ecology, but observing them in nature is hard! With metaHi-C, we detect freshwater phage–host pairs and link their infection dynamics to evolution. Together with @nadal-molero.bsky.social & Ana Martin-Cuadrado www.biorxiv.org/content/10.6...

A diet enriched in microbiome-fermentable substrates (more fruits and veggies) was shown to be associated with lower frailty and lower mortality risk in an older cohort (N>6000; 65+ y/o), with inflammation markers significantly mediating the dietary effect. link.springer.com/article/10.1...

Associations of dietary intake for gut microbiota with frailty risk and mortality in older adults - Journal of Translational Medicine

Journal of Translational Medicine - The associations between a diet that associated with a healthy gut microbiota and the risk of frailty, as well as mortality in older adults with frailty, remains...

link.springer.com

Fresh from our group, a new role for oxygen production in ammonia-oxidizing archaea: Oxygen production as an electron overflow pathway in ammonia-oxidizing archaea Congrats to Thomas Pribasnig for this great work! Check it out here: www.biorxiv.org/content/10.6...

Respiratory endosymbionts, that allow their ciliate hosts to breathe nitrate instead of (or in addition to) oxygen are frequent members of the wastewater microbiome. 🦠 🖥️🧬 Great to see this work by @louison-nicolas.bsky.social published in ISME coummuncations! doi.org/10.1093/isme...

Cosmopolitan marine bacteria facilitate a vast phytoplankton-derived sulfonate-based carbon flow through sulfoquinovosidases www.nature.com/articles/s41... #jcampubs 🌊

Microflora Danica: What can you learn from collecting and sequencing 10,000+ samples from a single country? Check out our new paper in @nature.com to find out. Incredible work led by Caitlin Singleton, Thomas B. N. Jensen, and Mads Albertsen from @aau.dk. 🦠🧫🧬 www.nature.com/articles/s41...

The Microflora Danica atlas of Danish environmental microbiomes - Nature

Microflora Danica—an atlas of Danish environmental microbiomes—reveals that although human-disturbed habitats have high alpha diversity, species reoccur, revealing hidden homogeneity.

nature.com

This nice summary by @michiwagner4.bsky.social makes it easy to fit my post in a few characters. Amplicon sequencing is still an affordable and quick way to get good insights into who lives in your samples. Our new primers make sure your vision is 20/20. Try em, and contact me if you have questions!

Michael Wagner@michiwagner4.bsky.social · 8mo ago

📢 New preprint on bioRxiv! We introduce V4-EXT, a revised 16S rRNA V4 primer set that dramatically improves detection of Patescibacteria and other undersampled lineages ✅ 938 samples ✅ More novel ASVs Primer choice matters 👀 www.biorxiv.org/content/10.1... @ppjevac.bsky.social #HuifengHu

Organizer Thilo Hofmann @cemess.bsky.social, contributions from 30+ @univie.ac.at scientists, incl @michiwagner4.bsky.social, @christinakaiser.bsky.social, Stephan Krämer, @jillianmpetersen.bsky.social, @ppjevac.bsky.social, @andreasrichter.bsky.social, and @loutsi.bsky.social from our cluster.

Distinct Microbial Communities Within and On Seep Carbonates Support Long-term Anaerobic Oxidation of Methane and Divergent pMMO Diversity academic.oup.com/ismej/advanc... #jcampubs

Metabolic biochemical models of N2 fixation for sulfide oxidizers, methanogens, and methanotrophs journals.asm.org/doi/full/10.... #jcampubs

Struc­tures of meth­ane-ac­tiv­at­ing en­zyme in elu­sive ar­chaea @mariecmueller.bsky.social @cuwelte.bsky.social @mpimarinemicrobio.bsky.social #methane #ANME #archaea #MCR #microbiology #biochemistry #StructuralBiology mpi-bremen.de/en/Structure...

✈️ Calling all grad students & postdocs in microbial ecology! Want to work, train, or collaborate abroad? ISME’s Scholar Mobility Fund can help you get there. 📅 Apply by 1 October 🌍 Open to applicants worldwide (esp. outside NA & Europe!) Details: isme-microbes.org/isme-scholar... #MicrobialEcology

One week to go! Apply by Sept 17 for the Full Professor position in Soil Ecosystems and Global Change at #CeMESS at the University of Vienna. More info: 🔗 ter.univie.ac.at/news-talks/n... @univie.ac.at #SoilEcosystems #WeAreHiring

We are hiring: Full Professor of Soil Ecosystems and Global Change

Soil ecosystems form the foundation of planetary health and play a critical role in climate feedback mechanisms. However, they are also vulnerable to the impacts of global change.

ter.univie.ac.at

Deadline approaching on Sept 17! We are hiring a Full Professor of Environmental Biogeochemistry of Metals at #CeMESS at the University of Vienna. Learn more: 🔗 edge.univie.ac.at/news-talks/n... @edge-vienna.bsky.social @univie.ac.at #EnvironmentalBiochemistry

We are hiring: Full Professor of Environmental Biogeochemistry of Metals

Human activities are driving rapid environmental change on a global scale, with pollutants playing a critical role in pushing planetary systems beyond safe limits. These changes have profound conseque...

edge.univie.ac.at

Trans rights are, in fact, human rights. Pass it on. 🏳️‍⚧️🫂

Dragons of Wales@dragonsofwales.bsky.social · 12mo ago

Trans rights are, in fact, human rights. Pass it on.

Biofilm lifestyle across different lineages of ammonia-oxidizing archaea academic.oup.com/ismej/advanc... #jcampubs

Holy cow - hyperspectral detection of bacteria at long distance.... "The bacteria were detectable outdoors under ambient light from up to 90 m in a single 4,000-m2 hyperspectral image taken using fixed and unmanned aerial vehicle-mounted cameras." 🤯 www.nature.com/articles/s41...

Hyperspectral reporters for long-distance and wide-area detection of gene expression in living bacteria - Nature Biotechnology

Hyperspectral reporter genes allow imaging of ground bacteria from aerial vehicles

nature.com

For our antimicrobial drug development efforts at the University of Vienna we are looking for input from physicians/clinicians who are familiar with treatment, diagnostics, infection control, etc. Your insights would be highly valuable! docs.google.com/forms/d/e/1F...

Survey on the clinical need for an ultra-narrow spectrum N. gonorrhoeae Antibiotic

Welcome and thank you for participating in this survey! We are the researchers in Boettcher's group at University of Vienna, who are developing the new antibiotic specifically targeting N. gonorrhoeae...

docs.google.com

Congrats to Nils Ganzwohl and Carolin Otersen for winning the Poster prizes at the 28th European Nitrogen Cycle meeting #ENC28

Our new preprint on mixotrophy of sponge-associated AOA via the uptake of branched-chain amino acids is online. www.biorxiv.org/content/10.1...

Jan Erisman closed the 28th European Nitrogen Cycle Meeting with a raw reminder: the nitrogen cycle is out of sync! Still, the meeting was full of inspiring research on all things N and some promising paths to reduce our impact! Big questions remain… #ENC28 univie.eventsair.com/enc2025/

See our new study examining how P additions in tropical rainforests affect tree growth and feedback to AMF and EMF communities. Very interesting links to EMF biomass production strategies. Great work led by Qingshui Yu 🍄‍🟫🍄 www.sciencedirect.com/science/arti...

Decadal nutrient addition reveals phosphorus limitation and its adaptive mechanisms in tropical rainforests

Tropical rainforests on low-phosphorus soils are highly biodiverse and productive, playing a crucial role in climate change mitigation. However, the d…

sciencedirect.com

Open postdoc position combining all the good things: #Ocean, #Nitrogen-cycling, #OxygenMinimumZones, #Biogeochemistry🧪🌊🔬 Check out this postdoc opening with my great colleague and friend #LauraBristow at #Uni #Gothenburg - part of our ERC Synergy #RECLESS: web103.reachmee.com/ext/I005/103...

2y biogeochem postdoc in ERC Synergy project RECLESS, with a focus on microbial nitrogen cycling in oxygen-depleted waters, based in Gothenburg, Sweden Info & apply here: web103.reachmee.com/ext/I005/103...

CeMESS is hiring! Join us at the Division of Terrestrial Ecosystem Research (@ter-cemess.bsky.social) as Full Professor of SOIL ECOSYSTEMS AND GLOBAL CHANGE. Collaborate across a leading institution in microbiome science and environmental research. 🔗 Learn more: cemess.univie.ac.at/news/detail-...

We are hiring: Full Professor of Soil Ecosystems and Global Change

Soil ecosystems form the foundation of planetary health and play a critical role in climate feedback mechanisms. However, they are also vulnerable to the impacts of global change.

cemess.univie.ac.at