noam
@prywes.bsky.social
Rubisco, protein engineering and photosynthesis at the Cambridge Biochemistry Department. pryweslab.org
New design for a DIY mini bioreactor from David Jordan in Cambridge - with accurate temperature control, gas composition monitoring, illumination, and pumps for feed and waste - ready for reactor networked arrays. Full details at: (notes.livingphysics.org/build_posts/...)
Machine learning-assisted directed evolution of plant Rubisco www.biorxiv.org/content/10.6...
Machine learning-assisted directed evolution of plant Rubisco
Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) is foundational to life on Earth, catalyzing carbon dioxide (CO2) fixation to generate biomass. However, Rubisco is a slow and inefficient enz...
biorxiv.org
A microbial growth-coupled platform for in vivo interrogation of Rubisco oxygenase activity www.biorxiv.org/content/10.6...
A microbial growth-coupled platform for in vivo interrogation of Rubisco oxygenase activity
Rubisco catalyzes the primary CO2-fixing reaction of the biosphere, yet its competing oxygenation reaction reduces net global carbon fixation and has resisted direct exploration in living cells. Here,...
biorxiv.org
Join our HFSP team on this amazing project! This will be an experimental postdoc position based in Amsterdam. There will be a more bioinformatic/evolutionary postdoc position based in Cambridge coming up soon. #socialfluids
Joris M. Koene@joriskoene.bsky.social · 4w agoAre you a biologist with experience in and enthusiasm for imaging and -omics techniques? Apply for working in Amsterdam (@vuamsterdam.bsky.social) on the exciting topic of socially transferred materials! 🐌 🐸 🐜🧬🔬 workingat.vu.nl/vacancies/po...
There are three different size systems (mini, midi, super) to cope with different scales, up to about a kilogram of fresh weight of liverwort per tray for the super size. Can take plants through the entire life cycle - great for plant spore production.
Form I and II Rubiscos Exhibit Temperature Dependent Carbon Kinetic Isotope Effects https://www.biorxiv.org/content/10.64898/2026.06.29.735352v1
Congratulations to Iva Tchasovnikarova who has been awarded the Lister Prize from @thelisterinstitute.bsky.social to expand her work on fundamental epigenetic pathways. Read more about Iva's research group and the Lister Prize: www.bioc.cam.ac.uk/news/iva-tch... #ListerPrize2026 #GurdonInstitute
Chloroplast genome editing of Rubisco boosts photosynthesis and plant growth @utokyo-yamori.bsky.social ! www.nature.com/articles/s41...
Chloroplast genome editing of Rubisco boosts photosynthesis and plant growth - Nature Communications
Rubisco has inherently low catalytic efficiency limiting crop productivity. Here the authors show that chloroplast genome editing can be used to generate substitutions in Arabidopsis rbcL that increas...
nature.com
Wow! Incredible work especially working in nitrogen fixing bacteria!
Kaçar Lab at UW-Madison@kacarlab.bsky.social · 2mo agoNew preprint! How do you engineer one of biology’s most complex enzymes without breaking it? We used evolution as a guide to map a protein interface in nitrogenase, screening >9,000 variants to reveal sequence-function rules for future nitrogen fixation engineering. www.biorxiv.org/content/10.6...
Congratulations to @claudiabonfio.bsky.social on her Harrison-Meldola Early Career Prize from @rsc.org for developing biophysical and supramolecular approaches to understand the origins and evolution of primitive cells. Read more about the prize and her lab: www.bioc.cam.ac.uk/news/claudia...
A bifunctional coiled-coil protein generates the membrane-within-condensate architecture of the CO2-fixing pyrenoid @mjonikas.bsky.social lab www.biorxiv.org/content/10.6...
A bifunctional coiled-coil protein generates the membrane-within-condensate architecture of the CO2-fixing pyrenoid
How membranes are integrated into biomolecular condensates is a fundamental question in cell biology. In the algal pyrenoid, an organelle responsible for one-third of global carbon fixation, CO2-deliv...
biorxiv.org
Excited to release #OpenSplice - the impact of >500,000 mutations on the alternative splicing of 600 human exons www.biorxiv.org/content/10.6... Led by @gioia-quarantani.bsky.social + funded by @opentargets.org @sangerinstitute.bsky.social @crg.eu #cshlsymp26 #RNA2026 @rnasociety.bsky.social
biorxiv.org
Partitioning of Rubisco activase into the pyrenoidal Rubisco condensate is mediated by a functional protein-protein interaction "https://doi.org/10.1038/s41467-026-70724-5" www.nature.com/articles/s41...
Partitioning of Rubisco activase into the pyrenoidal Rubisco condensate is mediated by a functional protein-protein interaction - Nature Communications
Algal photosynthesis relies on a biomolecular condensate known as the pyrenoid, which sequesters the cell’s Rubisco. The pyrenoid constituent Rubisco activase partitions to the condensate via an eleme...
nature.com
7/8 The full mechanistic model 🏛️: every step gated, every checkpoint structural. A complete blueprint for engineering better gene insertion tools. 🛠️
1/23 New preprint! Been thinking about the origin of cellular life. Specifically, how protocells (or *any* compartments) just aren't enough to support the evolution of increasingly complex cells. You gotta de-Darwinize the parts! The ribosome/genome do this. www.biorxiv.org/content/10.6...
biorxiv.org
With Eugene Koonin, we wrote a rather comprehensive review on the origin, evolution and organization of the #virosphere. We describe all 10 viral realms and the logic behind them, and so much more. Check it out! comptes-rendus.academie-sciences.fr/biologies/ar...
A de novo CO2 Reductase Featuring a Cysteine-Ligated Cobalt Porphyrin Cofactor https://www.biorxiv.org/content/10.64898/2026.05.07.723500v1
in vivo mutagenesis technique development continues to heat up, congrats to Max, @erika-alden.bsky.social and the whole team!
Pioneer Labs@pioneerlabs.bsky.social · 3mo agoThis tube is a library of bacteria with every single-base-pair genome mutation, all DNA-barcoded. 🧪Growth + barcode sequencing = data on millions of mutations. 📄 Report: www.biorxiv.org/content/10.6... Retweet if you want more data, and read on if you want to use the library! 🧵
Structural Insights into the Coupling Mechanism of Vectorial CO2 Uptake by DAB1 https://www.biorxiv.org/content/10.64898/2026.05.01.722082v1
Hornwort pyrenoids: a terrestrial exception with engineering lessons. @tarobison.bsky.social @fernway.bsky.social @laura-gunn.bsky.social @jcarlosvillarreal.bsky.social academic.oup.com/plphys/advan...
Validate User
academic.oup.com
This #EarthDay we're featuring Rockefeller's @flamholz.bsky.social, who studies how microbes contribute to #climatechange and how they could offer solutions to this problem via their metabolisms.
A Microbiologist Mines Bacterial Metabolism to Tackle Climate Change | The Scientist
Avi Flamholz leverages computational tools and microbial biochemistry to improve climate models and provide novel engineering solutions to address climate change.
the-scientist.com
We are recruiting a postdoc interested in using machine learning and AI to understand and control post-transcriptional gene regulation. Please apply and join us at @cambiochem.bsky.social ⏳ Closing date: 16 May 2026 🔗 www.jobs.cam.ac.uk/job/55254 Learn more about us: www.sblab.uk
bbc.com/audio/brand/... Now free for all archaea lovers to hear!
BBC Audio | In Our Time
Listen to the latest episodes of In Our Time on BBC Audio
bbc.com
New paper where we report that Npc2 - a shuttle-like lipid transfer protein - in yeast directly transports sphingolipids to the = vacuole membrane during formation of microautophagy-linked ordered domains #lipidtime www.sciencedirect.com/science/arti...
Transport of sphingolipids by yeast Npc2 supports phase separation of the vacuole membrane
The yeast vacuole membrane forms ordered microdomains that facilitate microlipophagy under nutrient limitation. We previously found that this process …
sciencedirect.com
Out recently in @natbiotech.nature.com — first authors @ddingding.bsky.social and @evangroover.bsky.social premier a cell-based method for understanding how #photosynthesis genes can be turned "up" or "down". Read here: ow.ly/KHWg50YyWbR
📣Huge preprint 🔔 Today we share something our group has been working toward for a long time, led by @lucasmoriniere.bsky.social We asked can we predict which receptor a phage targets from its genome sequence alone? For most phages, we couldn’t. So Lucas set out to do something I had only dreamed of.
Excited to see the major chapter of my PhD in print! We present a massively-parallel approach for screening CRISPR edits in plants to speed up agricultural test cycles + benchmark genomic LMs. Thanks to my fantastic co-authors and advisors. Link below!
Savage Lab@savagecatsonly.bsky.social · 4mo agoCan CRISPR edits enable precise tuning of plant gene expression? We think: yes. In our newest manuscript, we measured the effects of >30,000 CRISPR-like promoter mutations in sorghum protoplasts.
Can CRISPR edits enable precise tuning of plant gene expression? We think: yes. In our newest manuscript, we measured the effects of >30,000 CRISPR-like promoter mutations in sorghum protoplasts.
We have been cooking up this story for a while and we are excited to finally be able to share! Read on if you're interested in whole plant regeneration WITHOUT the application of hormones!
