If you miss science twitter, maybe you should join NeuroMethods on zulip (similar to slack but better): neuromethods.zulipchat.com/login/ If you have no idea what this is about, here's a brief blog post with the history: gcamp6f.com/2026/07/21/n...

Neuromethods

A space for neuroscientists to ask and answer questions about methods in Neuroscience (this replaces our previous Slack workspace). | Prospective users: you should be able to join with an academic ema...

neuromethods.zulipchat.com

Looking forward to #FENS2026 next week in Barcelona! I will present a poster on effects of locus coeruleus and arousal on astrocytes and neurons in hippocampus: PS06-09PM-201 PhD student Shinjini Ghosh will present a poster on a Theremin-based auditory virtual environment for mice: PS04-08PM-568

The @ptrrupprecht.bsky.social lab uses a really cool approach combining neuromodulator imaging, optogenetics, and two-photon calcium imaging to determine how locus coeruleus-mediated norepinephrine signaling influences different hippocampal cell types, including #astrocytes.

Peter Rupprecht@ptrrupprecht.bsky.social · last mo.

Happy to share that our paper is now out as a reviewed preprint : elifesciences.org/reviewed-pre...

Happy to share that our paper is now out as a reviewed preprint : elifesciences.org/reviewed-pre...

Peter Rupprecht@ptrrupprecht.bsky.social · 7mo ago

Excited to share our new preprint! We explore the link between the locus coeruleus (LC) and arousal for astrocytes, pyramidal cells, interneurons in the hippocampus. A fantastic collaboration with @sianduss.bsky.social @bohaceklab.bsky.social, and many others: www.biorxiv.org/content/10.6... 1/5

@ptrrupprecht.bsky.social can you give an estimate for the difference in baseline brightness between GCaMP8s and 6f?

I wanted to like QED's new 1% preprint ranking. I really did. But the more I looked at the data, the more uncomfortable it made me. Here is my full peer review. open.substack.com/pub/blekhman...

My Peer Review of The 1%

I wanted to like QED's new 1% ranking. I don't.

open.substack.com

The YouTube channel of @kurzgesagt.org made a great and accessible video on how memories are stored in our brains. I wrote a blog post to provide additional context and details for those interested in the view of a neurophysiology researcher: gcamp6f.com/2026/06/22/w...

What happens inside your brain when you form a memory?

Kurzgesagt is a Youtube channel that breaks down science topics for a broad audience. They have great videos, both in terms of narrative, animation, and scientific accuracy. A few days ago, the cha…

gcamp6f.com

I honestly hate watching talks made with AI. I hate it when speakers brag about how much time it saved them. If you have 80 people in your audience and they all watch you for 30 minutes, that's 40 hours of person-time. And you're telling us you're *proud* of how little you prepared? 🤷‍♀️

Two brain circuits. Same learning problem. Shared learning outcomes. Different dynamical implementations. Our work shows that learning is not a single canonical solution but can emerge through distinct population dynamics shaped by circuit architecture. Excited to share our latest preprint! 🧵1/12

bioRxiv Neuroscience@biorxiv-neursci.bsky.social · 2mo ago

Equivalent volitional learning emerges through circuit-specific population dynamics in motor cortex and hippocampus https://www.biorxiv.org/content/10.64898/2026.06.04.730137v1

We're thrilled to share this #Perspective @natneuro.nature.com on #astrocytes as a multilayered system of #functional #units that operate across spatial scales. 👉Available for reading here: rdcu.be/fk0B7 👉Full article here: www.nature.com/articles/s41...

The multiple scales of astrocytic functional units - Nature Neuroscience

Astrocytes modulate complex brain functions. These ubiquitous glial cells constitute a multilayered system of functional units that operate across multiple spatial scales, thereby increasing the degre...

nature.com

In this new blog post, I review some several recent studies on dendritic voltage and calcium imaging in hippocampus: gcamp6f.com/2026/05/19/d... Great work by @bhlee1117.bsky.social, Xiang Wu, @adamezracohen.bsky.social, Attila Losonczy, Balazs Rosza, Kevin Gonzalez, and others!

Dendritic imaging of pyramidal neurons in mouse hippocampus

Dendrites are the tree-like arborizations of neurons through which they receive input from other neurons. This compartmentalized anatomy has given rise to the idea that individual dendrites process…

gcamp6f.com

Delighted to share our discoveries about one of the brain's neurotransmitter systems: www.biorxiv.org/content/10.6... Together with colleagues at the @alleninstitute.org, we have learned a lot about a tiny cluster of neurons in the brainstem locus coeruleus (LC) that releases norepinephrine (NE). 1

Finally online @natmethods.nature.com www.nature.com/articles/s41... This was a fun collaboration with @finkryan.bsky.social @massecklab.bsky.social @tpatriarchi.bsky.social @martinfuhrmann.bsky.social @campbell-lab.bsky.social @jangrundemann.bsky.social 🧪🧠🔬🧬

PinkyCaMP: an mScarlet-based calcium sensor with enhanced brightness, photostability and multiplexing capabilities - Nature Methods

PinkyCaMP is a red genetically encoded calcium indicator with improved brightness and photostability. Derived from mScarlet, it does not exhibit photoswitching upon blue light illumination and is ther...

nature.com

Jonas Wietek@jwietek.bsky.social · 8mo ago

@massecklab.bsky.social @finkryan.bsky.social 🥳👏🧠🐭🔬🧪🩷

🧪 1/ New preprint from the lab: L5 pyramidal cell tLTD requires astrocyte Ca2+ and CB1 signaling www.biorxiv.org/content/10.6... We revisit a classic STDP result in visual cortex

1/8. New preprint! Using fUSi in head-fixed mice🐭, we found that arousal events trigger a brain-wide wave of activity 🌊🧠. Surprisingly, this pattern was preserved during opto manipulations of the locus coeruleus, pointing to a minor role for noradrenergic tone. www.biorxiv.org/content/10.6...

First whole-brain recording of social sound processing in a vertebrate. Surprises start in the hindbrain; thalamus gates conspecific calls; male and female brains diverge downstream. Work by @joerghenninger.bsky.social, @mh123.bsky.social sky.social and team. www.biorxiv.org/content/10.6...

Two-photon calcium imaging at 24,000 lines/s, with the resonant axis spanning 4x what other systems can do. Inertia-free. Diffraction-limited. No tradeoffs. Che-Hang Yu developed a 4x angle multiplier for laser scanning. His paper is out today: opg.optica.org/optica/fullt... 1/n #fluorescenceFriday

First time patching in months, y'all know what that means: I am about to spend the next few hours discovering fun and novel ways in which an ephys setup can break!

Interested in deep learning-based spike inference with Cascade? We re-implemented the code in Torch -previously in Tensorflow - for better compatibility. Blog: gcamp6f.com/2026/02/17/c... GitHub: github.com/PTRRupprecht...

CascadeTorch: a PyTorch version of Cascade for spike inference

I’m glad to share a PyTorch-based implementation of spike inference from calcium imaging data: CascadeTorch, now available on GitHub. The original Cascade repository remains fully supported. …

gcamp6f.com

A new preprint introduces aDISCO, a DISCO-based clearing approach that makes whole archival FFPE human tissues transparent and antibody-compatible, enabling true 3D light-sheet histology across brain & multiple organs at cellular resolution. Aguzzi & Helmchen teams. www.biorxiv.org/content/10.1...

But then where do the branch-specific variations in voltage come from? We found that in apical dendrites, small subthreshold depolarizations *preceding* a complex spike partially predict voltage (and Ca2+) amplitude *during* the event. This suggests conjunctive amplification of inputs and spikes.

What's the relation between voltage and calcium in dendrites? Xiang Wu studied this in CA2 hippocampal pyramidal cells in behaving mice. www.biorxiv.org/content/10.6...

Excited to share our new preprint! We explore the link between the locus coeruleus (LC) and arousal for astrocytes, pyramidal cells, interneurons in the hippocampus. A fantastic collaboration with @sianduss.bsky.social @bohaceklab.bsky.social, and many others: www.biorxiv.org/content/10.6... 1/5

Still have to read it carefully with all methods and details, but it's a candidate for my favorite paper of 2026! Very cool, and important work to understand how neurons and dendrites work!

@adamezracohen.bsky.social · 7mo ago

What is the computational role of dendritic excitations? Byung Hun Lee and team mapped voltage dynamics throughout the dendritic trees of CA1 pyramidal neurons in mice navigating in virtual reality. www.biorxiv.org/content/10.6...

What would it really mean to achieve experimental goals that currently seem out of reach: a new blog post. Part I - connectomics gcamp6f.com/2025/12/27/a...

Annual report of my intuition about the brain (2025, part I)

How does the brain work, and how can we understand it? To approach this big question from a broad perspective, I want to report on some ideas about the brain that marked me most over the …

gcamp6f.com

New preprint from the lab! 🚀 We find that hippocampal OLM interneurons provide a circuit-level inhibitory feedback signal that dynamically controls when and where behavioral timescale synaptic plasticity can occur. Feedback welcome!

bioRxiv Neuroscience@biorxiv-neursci.bsky.social · 8mo ago

Dendrite-targeting OLM interneurons regulate the formation of learning-related CA1 place cell representations https://www.biorxiv.org/content/10.64898/2025.12.21.695825v1

What is the computational role of dendritic excitations? Byung Hun Lee and team mapped voltage dynamics throughout the dendritic trees of CA1 pyramidal neurons in mice navigating in virtual reality. www.biorxiv.org/content/10.6...

Thankful for this blog piece by @ptrrupprecht.bsky.social on the Dirigo project. Glad you liked the posts! gcamp6f.com/2025/11/14/d...

Dirigo: a future Python alternative to Scanimage?

Over the last 20 years, many microscopes that are capable of resonant scanning two-photon microscopy have converged on using ScanImage, a powerful software package with many strengths but also two …

gcamp6f.com

Independent NeuroScience Services INSS was registered as a company on 14th November 2016, which means it's our 9th birthday today! As a birthday present we've treated ourselves to an SfN exhibitor booth for the first time ever. Come and say hello at Booth #3327

New blog post, where I highlight a few interesting papers on astrocyte ultrastructure and function, with papers from the labs of Moritz Helmstädter @mhlab.bsky.social, Kristen Harris, Andrea Volterra, Karin Pernet-Gallay, and Hailan Hu: gcamp6f.com/2025/11/14/i...

Interesting papers on astrocyte ultrastructure and function

Astrocytes are brain cells that are often completely overlooked and dismissed or, in the opposite extreme, presented as mysterious devices that somehow solve all problems of computational neuroscie…

gcamp6f.com