Nice use of ❤️Chrimson-PdCO💜 to investigate the synaptic connectivity and its topographical organization between the BLA and mPFC along the rostro-caudal axis. 🧠🧪 doi.org/10.64898/202... PdCO resources: docs.google.com/spreadsheets...
Jonas Wietek
@jwietek.bsky.social
Synapse modulation, biophysics, optogenetics, opsins, optoGPCRs & channelrhodopsins... garden stuff, photography, cats, cycling, parenting...still postdocing. Patch-clamper with imaging ambitions https://scholar.google.com/citations?user=4xDsEF4AAAAJ&hl=en
A long-standing question in neuroscience is why the eCB system uses two ligands for the same receptors and how their release differs. To address it, we’re happy to share a pair of selective GRAB sensors that detect 2-AG and AEA, respectively, in real time. biorxiv.org/content/10.6...
Excited to share our work now in Nature Methods! We wrote this manuscript to help others better analyze and interpret calcium imaging data. As a central aspect, we show why nonlinear behavior of calcium indicators matters in practice. Very happy about this work!
Nature Methods@natmethods.nature.com · 3w agoAn Analysis from @ptrrupprecht.bsky.social et al investigates the behavior of GCaMP8 variants in spike inference using different algorithms. www.nature.com/articles/s41...
www.biorxiv.org/content/10.6... nice stuff!
A highly Ca2+-permeable channelrhodopsin from the ancyromonad Nutomonas longa enables optogenetic control of Ca2+ signaling
Ca²⁺ is a ubiquitous regulator of cellular function, linking electrical activity to gene expression, secretion, metabolism, and synaptic plasticity. Yet, tools for its direct, time-resolved optical ma...
biorxiv.org
light-activated tetanus neurotoxin (LATeNT) by the @aliceyting.bsky.social lab. Includes a comparison with the light-switchable PdCO (www.nature.com/articles/s41...), showing a long lasting inhibition for 1h hour post illumination. www.nature.com/articles/s41...
Light-activated tetanus neurotoxin for conditional proteolysis and inducible synaptic inhibition in vivo - Nature Methods
LATeNT is a light-activatable form of tetanus neurotoxin that can be used for spatiotemporally precise inhibition of exocytosis in neurons and other cells. It can inhibit synaptic transmission in vivo...
nature.com
What if part of Huntington's disease wasn't just damage, but a circuit stuck in the wrong pattern? Our new paper, out today in Nature, tests that idea.
Whats going on @fens.org ? Please lift this restriction. This tool was of incredible value!!!
Nikolas Karalis@nikolaskaralis.bsky.social · 3mo agoWith #FENS2026 just around the corner, our unofficial @fens.org abstract explorer is back: fens2026.neuronaldynamics.eu • Search abstracts by author, keyword, affiliation, and more • Discover related work using abstract similarity • Save favorites, add notes and tags, and build your own itinerary
My department, Neurobiology and Biophysics at the University of Washington (nbio.uw.edu), is hiring a new tenure-track Assistant Professor, application deadline 09/30/26. It's a broad search — molecular to organismal, all of neuroscience/physiology/biophysics welcome. apply.interfolio.com/187735
congrasts @labnir.bsky.social for getting an @erc.europa.eu Proof of Concept '26
@ptrrupprecht.bsky.social can you give an estimate for the difference in baseline brightness between GCaMP8s and 6f?
The Mandalorian (Book of Boba Fett): This Is The Way
ALT: The Mandalorian (Book of Boba Fett): This Is The Way
static.klipy.com
Harmit Singh Malik@harmitmalik.bsky.social · 2mo agoI know it seems like a heretical idea, but one way to figure out if a preprint is really interesting (to you) is... to read the preprint. Then, in the spirit of sharing, send a note to authors: a simple kudos or a more detailed question. I do this a dozen times a year. It's awesome. Try it.
congratulations @oyiz.bsky.social @weizmann.ac.il for securing and @erc.europa.eu AdG on Neural circuits for Early-Life Social Trajectories (NEST)
Excited to share our first red ACh sensor! 🔴 We developed GRAB_rACh1h, the first genetically encoded red fluorescent sensor for recording acetylcholine dynamics in vivo. (1/3)
With #FENS2026 just around the corner, our unofficial @fens.org abstract explorer is back: fens2026.neuronaldynamics.eu • Search abstracts by author, keyword, affiliation, and more • Discover related work using abstract similarity • Save favorites, add notes and tags, and build your own itinerary
A red-emitting, genetically encoded indicator for two-photon voltage recording in vivo www.biorxiv.org/content/10.6...
A red-emitting, genetically encoded indicator for two-photon voltage recording in vivo
Genetically encoded voltage indicators (GEVIs) enable minimally invasive, cell‑type‑specific optical measurements of neuronal membrane potential with millisecond temporal resolution. Red‑shifted GEVIs...
biorxiv.org
Excited to announce big steps for electrical imaging in the brain! In 4 preprints, we and collaborators present 2 new GFP-based voltage sensors: ASAP6c for high-throughput spike recordings, and ASAP7y for subthreshold 1P and 2P imaging bit.ly/4x3k3kX bit.ly/3RKZhq3 bit.ly/4o4fKl3 bit.ly/4uHXuk3 🧵
