Myst (1993)

Gotta believe in your product!

My first corresponding author publication in @jacs.acspublications.org Very proud and feeling like a bona fide chemist pubs.acs.org/doi/full/10....

Apply by Feb. 3 to become a Janelia Group Leader! Group Leaders drive breakthroughs & experimental approaches in imaging, molecular engineering, protein chemistry, mass spectrometry, & methods that don't yet exist. Learn more: janelia.org/groupleader 🧪

Consider submitting your peptide research to a special joint issue of JOC and Biochemistry on the Chemistry and Biology of Peptides! axial.acs.org/biology-and-...

Call For Papers: Chemistry and Biology of Peptides | ACS Publications Chemistry Blog

This Special Issue will celebrate the chemistry and biology of peptides, from synthesis, to structure and modifications, to biology and applications, and finally to degradation. Submit your manuscript...

axial.acs.org

Bitopic ligands comprise a #pharmacophore & a linked moiety that bind to separate sites. @rche54.bsky.social &co show that #GPCRs that bind small molecules can be potently activated by #bitopic #nanobody -ligand conjugates to selectively target pairs of GPCRs @plosbiology.org 🧪 plos.io/4p3VOyH

So cool to read this commentary that covers our recent work! journals.plos.org/plosbiology/...

Nanobody-drug conjugates for targeting specific GPCR pairs

Bitopic ligands that engage two distinct binding sites offer exciting opportunities for finely tuned control of G protein-coupled receptor signaling. This Primer explores a recent study in PLOS Biology that reports novel nanobody-small molecule conjugates and demonstrates their logic-gated activity at co-expressed receptor pairs with improved signaling profiles.

journals.plos.org

Ross Cheloha@rche54.bsky.social · 9mo ago

So pleased to share the final version of this work published in @plosbiology.org ! journals.plos.org/plosbiology/...

So pleased to share the final version of this work published in @plosbiology.org ! journals.plos.org/plosbiology/...

Development of bitopic nanobody-ligand conjugates targeting G protein-coupled receptors and exhibiting logic-gated signaling

Bitopic ligands are comprised of a pharmacophore and a linked moiety that bind to separate sites. This study shows that G-protein coupled receptors (GPCRs) that bind small molecules can be potently ac...

journals.plos.org

Ross Cheloha@rche54.bsky.social · last yr.

Proud to share this work from my group in the intramural research program at NIH! We describe a new kind of conjugate made from a small molecule adenosine analog linked to a GPCR targeting nanobody to make antibody drug conjugates that potently and specifically activate signaling (1/n) 🧪 #chembio

Read this in a paper and it was totally new to me (after 18 years in biochemistry research): “Peptides translated from the sense and antisense strands of DNA interact specifically, analogous to the complementary interactions between DNA double strands.” Is this widely studied/actually true?? 🤯

Faculty opening in Cornell Chemistry at the Assistant Professor level in the area of chemical biology, broadly defined! academicjobsonline.org/ajo/jobs/30338 #chemjobs

Last day at #ENDO2025 was great! Amazing symposium on bone and calcium disorders 🦴 by @rche54.bsky.social @MichailLionakis @MichaelMannstadt with great talks on GPCRs and nanobodies, JAK inhibitors in APECED syndrome & new PTH therapies 🤩

This Wednesday and Thursday is the VIRTUAL Nebraska Drug Discovery and Development Pipeline Symposium! Registration is still open! Plenary speakers include Brian Kobilka and Virginia Man-Yee Lee. #chemsky, you should attend! research.unl.edu/blog/annual-...

Annual Nebraska Drug Discovery and Development Pipeline symposium June 25-26

World-renowned experts will offer their insights during the fifth annual ND3P Symposium, set for June 25-26. The symposium will be virtual and highly interactive.

research.unl.edu

Check out the final published product in Journal of Biological Chemistry! www.jbc.org/article/S002...

Evaluation of Alphafold modeling for elucidation of nanobody-peptide epitope interactions

Models of Ab-antigen complexes can be used to understand interaction mechanisms and for improving affinity. This study evaluates the use of the protein structure prediction algorithm AlphaFold (AF) fo...

jbc.org

Ross Cheloha@rche54.bsky.social · last yr.

Excited to share a preprint covering a collaborative project from my group, the Bewley lab (NIDDK), and the Pierce lab (University of Maryland) describing our first foray into assessing Alphafold for predicting peptide-nanobody interactions (1/n) www.biorxiv.org/content/10.1...

Excited to see this out in @science.org today!! www.science.org/doi/10.1126/...

Intracellular protein editing enables incorporation of noncanonical residues in endogenous proteins

The ability to study proteins in their native cellular context is crucial to our understanding of biology. In this work, we report a technology for intracellular protein editing, drawing from split in...

science.org

George Burslem@gburslem.bsky.social · 2y ago

Ever wanted to edit a protein sequence inside a living mammalian cell? Maybe to site specifically incorporate a non-canonical residue? Perhaps you’d like to do a pull down without an antibody? Or use a small molecule fluorophore to image an endogenous protein? 1/4

Next up, a collaboration with Ross Cheloha's group (@rche54 @rche54.bsky.social) and @MitchellHo leveraging a novel self-labeling approach for site-specific modification of nanobodies for biological applications. doi.org/10.1002/advs... 3/5

It's been a while since I posted on some of the ☢️ #radiopharmaceuticals ☢️ work we've been brewing. Let's fix that... 🧵👇 1/5

Great news: NIH postbac program is recruiting again! If your grad school plans were affected by program cutbacks or admissions freezes this year I highly encourage you to apply, this could be a perfect opportunity. Please repost. www.training.nih.gov/research-tra...

Check out our new review on induced proximity modalities (#TPD and a lot more!) aimed cell surface targets @naturebiotech.bsky.social, spearheaded by postdocs Nick Till and "Rahm" Ramanathan www.nature.com/articles/s41...

Induced proximity at the cell surface - Nature Biotechnology

Technologies to modulate induced proximity at the cell surface advance the manipulation of diverse biological responses.

nature.com

The findings suggest that optimizing input parameters and refining competition-based modeling approaches may enhance predictive accuracy for antibody-antigen interactions. Intriguing.

Excited to share a preprint covering a collaborative project from my group, the Bewley lab (NIDDK), and the Pierce lab (University of Maryland) describing our first foray into assessing Alphafold for predicting peptide-nanobody interactions (1/n) www.biorxiv.org/content/10.1...

Evaluation of Alphafold modeling for elucidation of nanobody-peptide epitope interactions

Models of Ab-antigen complexes can be used to understand interaction mechanisms and to improve affinity. This study evaluates the use of the protein structure prediction algorithm AlphaFold (AF) for e...

biorxiv.org

Proud to share this work from my group in the intramural research program at NIH! We describe a new kind of conjugate made from a small molecule adenosine analog linked to a GPCR targeting nanobody to make antibody drug conjugates that potently and specifically activate signaling (1/n) 🧪 #chembio

bioRxivpreprint@biorxivpreprint.bsky.social · last yr.

Nanobody-based conjugates targeting small molecule-binding GPCRs and exhibiting logic-gated signaling https://www.biorxiv.org/content/10.1101/2025.03.07.642093v1

Nanobody-based conjugates targeting small molecule-binding GPCRs and exhibiting logic-gated signaling https://www.biorxiv.org/content/10.1101/2025.03.07.642093v1

One good thing I saw today

Proud to share this publication about the development of a new method for visualizing tumors! This work resulted from a close collaboration among 3 groups. (1/n) 🧪@freddyeescorcia.bsky.social advanced.onlinelibrary.wiley.com/doi/10.1002/...

Generation of Site‐Specifically Labeled Affinity Reagents via Use of a Self‐Labeling Single Domain Antibody

Several methods have been used to label antibodies to facilitate visualization of cognate targets. Here, we leverage a new variety of conjugation chemistry, based on a crosslinking nanobody, to label...

advanced.onlinelibrary.wiley.com

Our NCATS research program has a history of collaborating and partnering to get treatments to patients. One of my first goals as SD was to capture and share our success in one place - and here it is! 4 approvals and 56 INDs to date - not bad for 12 years old! ncats.nih.gov/research/our...

Overjoyed and honestly relieved to share a Chem Rev magnum opus from Brian Chen & Julia Li titled Synthetic Lipid Biology! In it, we organize efforts across numerous fields to create, manipulate, and analyze lipids and biomembranes. Check it out! doi.org/10.1021/acs.... #lipidtime #chemsky #chembio

🎄Xmas gift!🎁Joint work with #OliverHantschel in @naturecomms.bsky.social 💥! Huge kudos 💪🏼😻 Nina 👩‍🔬 for leading as 1st author & Frank 👨🏼‍🔬 for training her like a pro❤️ Small delay😅 -my👵broke her hip🤦🏼‍♀️-loved ones always come first❤️surgery went good💪🏼🤩Let's celebrate🥳🥂! #ChemBio www.nature.com/articles/s41...

Development of mirror-image monobodies targeting the oncogenic BCR::ABL1 kinase - Nature Communications

In this work, the authors develop mirror-image monobodies (Mb) made of D-amino acids against the BCR::ABL1 SH2 domain with high binding affinities. The heterochiral Mb-SH2 structures reveal an unusual...

nature.com

1/ In two back-to-back papers, we present our de novo TRACeR platform for targeting MHC-I and MHC-II antigens TRACeR for MHC-I: go.nature.com/4gcLzn5 TRACeR for MHC-II: go.nature.com/4gj5OQk

A thought provoking scenario: what would happen if "mirror" (reverse chirality) microbe were created and released? The time estimate (10 years) seems long to me as there's been incredible progress in making mirror enzymes. You don't need much of a few mirror enzymes for a self sustaining cascade..🧪

Confronting risks of mirror life

Broad discussion is needed to chart a path forward.

science.org

Are you a senior PhD student or postdoc? Do you have a cool science story to tell? Do you want to visit Buffalo when the weather is perfect? Then apply for the Emerging Leaders Seminar Series at Roswell Park! DM or email me with questions. (RT please!) www.roswellpark.org/education/pr...