Excited to see this collaboration now in print. Dye chemistry meets AI-designed proteins. Three small, orthogonal tags; lifetime modulation; split versions. Request the free dyes at dyes.janelia.org www.science.org/doi/10.1126/...

De novo design of orthogonal far-red, orange, and green fluorophore-binding proteins for multiplexed imaging

Fluorescent proteins and small-molecule dyes offer complementary advantages for biological imaging: proteins are amenable to genetic tagging, whereas dyes provide superior brightness and photostabilit...

science.org

Is this satire?

In Preparation@inprep.bsky.social · 3w ago

OK--is this satire??? The fact that we can't tell anymore is..... inpreparation.substack.com/p/opinion-i-...

Opinion: I Was Not Allowed To Type Prompts Into ChatGPT During My Chalk Talk And This Is Discrimination

By Dr. Rachel Simmons, Postdoctoral Fellow, Stanford University

inpreparation.substack.com

Out in @natmethods.nature.com: More dyes. They work. Quite well. And blink. Pick the one that fits your target, your technique, and your labeling density. With too many collaborators and institutes to list, but anchored at @hhmijanelia.bsky.social www.nature.com/articles/s41...

A series of spontaneously blinking dyes for super-resolution microscopy - Nature Methods

A series of spontaneously blinking dyes in the far-red range facilitate single-molecule localization microscopy. These dyes vary in their blinking properties and can be matched to the applications and...

nature.com

The best two years you can spend before graduate school! Get the hands of a 4th-year grad student contributing to Open Science: bit.ly/47mU5xx Or join an interdisciplinary AI/biology team (don't worry, the AI still needs you to do the actual experiments): bit.ly/4taqzni Both at Janelia!

Research Technician - Tool Translation Team

Primary Work Address: 19700 Helix Drive, Ashburn, VA, 20147 Current HHMI Employees, click here to apply via your Workday account. Summary: We are seeking a chemist to join the Tool Translation Team, "...

bit.ly

Excited to share our new paper and lipid- and protein-directed photocatalytic labeling method (POCA) just out in @natchembio.nature.com. tinyurl.com/2kcxuvvv. Big congrats to first author Andrew Becker and the whole team for launching our lab into the wild world of singlet oxygen interactomics.

Thanks, as always, to @rhodamine110.bsky.social for hooking us up with buckets of JaneliaFluor-HaloTag ligand dyes.

What is the computational role of dendritic excitations? Byung Hun Lee and team mapped voltage dynamics throughout the dendritic trees of CA1 pyramidal neurons in mice navigating in virtual reality. www.biorxiv.org/content/10.6...

Got the last word in this great perspective on the state of fluorescent indicators in @nature.com by @dianakwon.bsky.social www.nature.com/articles/d41...

How genetically encoded sensors have lit up neuroscience

Tools that track specific molecules in neurons have enabled researchers to probe previously unexplored aspects of neurobiology — although important caveats remain.

nature.com

Registration for THE chemical biology conference of 2026 is now open! EMBO ChemBio 2026 in Heidelberg DeGrado, Arikin, Picotti (Keynotes). @lmkdassama.bsky.social @brianliau.bsky.social @rhodamine110.bsky.social @benlehner.bsky.social @alitavassoli.bsky.social www.embl.org/about/info/c...

Chemical biology 2026

embl.org

Cool paper using a new JF derivative to map synaptic proteins. And AI didn't like the generality of our general synthetic methods section (that we've used for years), so we expanded them from 2 succinct paragraphs to 1.5 gloriously unreadable pages. Better than Ambien. www.nature.com/articles/s41...

Single-cell synaptome mapping of endogenous protein subpopulations in mammalian brain - Nature Communications

Synapses are diverse within a single neuron. Here, the authors present a method for single-cell synaptome imaging of endogenous protein subpopulations in the mouse brain, enabling spatial mapping of s...

nature.com

Found it!

A fantastic paper by my @hhmijanelia.bsky.social colleague and friend Heejun Choi (who is on the job market!). Another elegant use of the Janelia Fluor dyes for cellular imaging. www.nature.com/articles/s41...

Secretome translation shaped by lysosomes and lunapark-marked ER junctions - Nature

Live-cell imaging of mRNA encoding secretome proteins and translated nascent peptide markers show that secretome translation occurs at endoplasmic reticulum junctions near lysosomes, requires lun...

nature.com

Check out this cool method to quantify EV diffusion in vivo using the JF dyes! Awesome work by Ema Cocucci and coworkers. urldefense.com/v3/__https:/...

Exploring the Spatial Limits of Extracellular Vesicles‐Mediated Intercellular Communication

Extracellular vesicles (EVs) are biological nanovectors that retain molecular signatures of their cells of origin and mediate intercellular communication, resulting in ideal platforms for the develop....

urldefense.com

Outlook is autocorrecting "on:off ratio" to "on😯ff ratio" and I love it so much.

Very cool collaborative work between multiple groups across three institutions. Shoutout to @rhodamine110.bsky.social and his crew along with longtime @the.3i.social community @so-lets-kilab70.bsky.social for deploying 3i's #latticelightsheet for some particularly elegant chromatin microscopy. 🔥🔬🧠🧬🥽

David Solecki@so-lets-kilab70.bsky.social · 9mo ago

When chemistry meets neurons! Game-changing paper on multifunctional fluorescent ligands. Luke and Pratik built the nerdy toys; we got to play! Jason Vevea nailed biotin-HTL for mito magic. My lab rocked the JQ1 HTL to shuffle chromatin in mins. 1st collab win for our Neuronal Cell Biology Division.

Just upgraded to a Claude Enterprise account (thanks HHMI) and was playing with the Molecule studio artifact. Here is rhodamine 110, my BlueSky username. Looks like our jobs are safe for now!

A new, nerdy paper. We figured out (some) of the rules underlying cell-permeability of probes and designed ligands that light up, grab, and move proteins around. Awesome @hhmijanelia.bsky.social x @uwmadison.bsky.social x @stjuderesearch.bsky.social collaboration! www.pnas.org/doi/10.1073/...

📢 Applications are open for the 4th installment of our ChemTools conference. We'll explore: 🧪 Platforms & tools for analyzing cell/tissue networks ⚡ Methods to perturb biological networks 🔍 Applications that yield new insights 🛏️ Lodging + meals covered ➡️ Apply by Dec 5: https://janelia.news/CHM26

PSA: If you are a #ChemDraw user on the Mac, they changed the font for the ACS Document 1996 style in the recent past; Helvetica to Arial. Watch out for vestigial Helvetica characters in figures, which some journal submission platforms *really* don't like.

a rainbow and a star with the words " make you know " on it

ALT: a rainbow and a star with the words " make you know " on it

media.tenor.com

My debut on BlueSky! Here’s how we harvest the firing of neurons to see them better in the living brain! 👇 www.nature.com/articles/s41... Grateful for the amazing dyes from @rhodamine110.bsky.social and insightful suggestions from @adamezracohen.bsky.social !

Imaging neuronal voltage beyond the scattering limit - Nature Methods

ALI is an approach for analyzing voltage imaging data that is inspired by algorithms used in super-resolution microscopy. It allows resolving the activity of single neurons in densely labeled populati...

nature.com

Nature Methods@natmethods.nature.com · last yr.

ALI, a method for analyzing voltage imaging data, allows resolving the activity of single neurons in densely labeled populations in scattering conditions. www.nature.com/articles/s41...

The sophomore outing for this revelatory conference is here! Join us for #OPTSRC for the latest in optical probes, which are crucial for biological imaging techniques. You'll meet organizers like @rhodamine110.bsky.social and more. View the agenda and save your space: buff.ly/LIRRF15

We're looking for 1-2 enthusiastic early career chemists to join our Open Chemistry team at @hhmijanelia.bsky.social. Come help us create amazing dyes and share them with the world. Please share with the best recent/soon-to-be B.S. chemists you know! bit.ly/42w8C6N

Research Technician - Open Chemistry

Primary Work Address: 19700 Helix Drive, Ashburn, VA, 20147 Current HHMI Employees, click here to apply via your Workday account. Summary: Open Chemistry is a collaborative research group whose missio...

bit.ly

Thanks Luke! Grateful for your good work kind sir. Thirty years ago, I remember crowding around the lightbox in awe with my Pharmingen R&D colleagues at the glowing BioColors vectors we had grown up after licensing from Roger Tsien's Lab down the street at UCSD where I was about to graduate. 🔬🧪🥽🔥🌈

a picture of luke skywalker with the words luke !!! above him

ALT: a picture of luke skywalker with the words luke !!! above him

media.tenor.com

I’ve been a dye chemist for a quarter century! 25 years ago today I walked into this funky little company in Eugene, OR called Molecular Probes. My first task was making Alexa Fluor 633 and I never looked back. I am grateful for all my teachers and mentors along the way.

Honored to help enable two back-to-back papers in @natureneuro.bsky.social; first DELTA www.nature.com/articles/s41... with collaborators at @hhmijanelia.bsky.social and then EPSILON www.nature.com/articles/s41... with @adamezracohen.bsky.social; @dyerfulchymist.bsky.social is always in the mix!

DELTA: a method for brain-wide measurement of synaptic protein turnover reveals localized plasticity during learning - Nature Neuroscience

Researchers developed DELTA, a method for brain-wide measurement of synaptic protein turnover with single-synapse resolution, providing a powerful tool to localize and study mechanisms underlying syna...

nature.com

🥼 Janelia researchers led by @boazmohar.bsky.social @nspruston.bsky.social @rhodamine110.bsky.social & @svoboda314.bsky.social @alleninstitute.bsky.social have developed a new imaging tool that maps brain-wide changes in neuronal connections. 🧠 🔗 www.janelia.org/news/new-ima...

A little nugget of whimsy: Apple Music has these “lyrics” for the Star Wars main theme (from ESB).

You know you're getting old when your Organic Chemistry Lab TA training partner from the first week of grad school is now an editor at @acs.org (does this mean I have to click 'Accept' @pomerantz.bsky.social?)

How it started: About eight years ago Bob Tjian ("Tij") and I sat in the back of cab in San Francisco and discussed the crazy idea of using live-cell single-molecule tracking for high-throughput drug screening. How it's going: