Tessera
Folgen

Alex Kingston

@alexjkingston.bsky.social

Wellcome trust PhD student @scicambridge.bsky.social @imperiallifesci.bsky.social working on tissue mechanics and cell fate Reposting papers so I can find them later :)

3 Posts38 Follower178 Folgt
Stefan Schoenfelder@stefanschoenfelder.bsky.social · 3w ago

Very happy to see this story finally out in @natbiotech.nature.com: www.nature.com/articles/s41... Fantastic collaboration with @lancasterlab.bsky.social on how to identify and ‘fix’ differentiation-compromised human pluripotent stem cells. @babrahaminst.bsky.social @mrclmb.ac.uk 🧵 below

Reversible epiblast regionalization determines differentiation potential of human pluripotent stem cells - Nature Biotechnology

The differentiation variability of human pluripotent stem cell lines is diagnosed and corrected.

nature.com

Jay Shendure@jshendure.bsky.social · 4w ago

Preprint: biorxiv.org/content/10.6... This is the product of many years of effort, and particularly amazing summer teamwork by Qi Yu, Haedong Kim, @seidels.bsky.social @cxqiu.bsky.social and many others from Shendure Lab & SeaHub (a @alleninstitute.org @biohub.org @uwmedicine.bsky.social colab) 2/n

In vivo reconstruction of the cell lineage history of a developing mouse with DNA Typewriter, from zygote to late organogenesis

The complete cell lineage of C. elegans, mapped over four decades ago, was tractable because the animal is small, transparent, and lineage invariant. Most animals are none of these, having orders of m...

biorxiv.org

Stirling Churchman@stirlingchurchman.bsky.social · last mo.

RNA-seq tells us how much RNA is present in the cell. But to understand gene regulation, we need to easily measure the synthesis and decay rates driving this abundance. We introduce AIR-seq: analog intrinsic recoding sequencing. (1/6) www.biorxiv.org/content/10.6...

Analog intrinsic recoding measures RNA dynamics without chemical conversion

Steady-state RNA abundance measurements mask the synthesis and decay rates that shape gene expression. Analog intrinsic recoding sequencing (AIR-seq) repurposes the base-pairing properties of N4-hydro...

biorxiv.org

Alex Kingston@alexjkingston.bsky.social · last mo.

Pleased to say that the first half of my PhD project is now out in preprint format! Using cortical organoids to model the mechanical transitions involved in early neurogenesis, we find that nuclear mechanotransduction is a crucial regulator of the timing and outcome of neuronal differentiation:

Organoids reveal niche-specific mechanotransduction-guided human cortical patterning and cell fate acquisition

The highly dynamic mechanical environment of the developing cerebral cortex has the potential to encode information vital for neuronal differentiation. Changes in local biophysical properties have bee...

biorxiv.org

James Briscoe@jamesbriscoe.bsky.social · 2mo ago

New in @dev-journal.bsky.social: how tissue shape tells cells what to become Using human stem-cell neuruloids, we show how that physical boundaries encode positional information that drives cell fate patterning in a model of trunk formation A thread 🧵 journals.biologists.com/dev/article/...

Boundary constraints can determine pattern emergence

Summary: A reaction-diffusion mechanism recapitulates pattern emergence in an in vitro model of neuromesodermal progenitors under a variety of boundary and geometrical perturbations.

journals.biologists.com

Cell Reports@cp-cellreports.bsky.social · 3mo ago

A human corticospinal organoid-slice connectoid model informs enhancer strategies for post-injury axon regrowth

A human corticospinal organoid-slice connectoid model informs enhancer strategies for post-injury axon regrowth

Gibbons et al. present a human corticospinal organoid slice-based connectoid model, with physically separate but functionally coupled cortical and spinal regions. Allowing cell-type-specific observations, the platform uncovers a developmental maturation-dependent decline in human axon regeneration and a transcriptional program intrinsic to cortical projection neurons that, when targeted, promotes repair post-injury.

dlvr.it

Bianca Dumitrascu@bimidu.bsky.social · 3mo ago

How are two embryos alike? As we collect spatio-temporal microscopy data, we want to quantify variability in the timing of key developmental events. Alignment of multiple recordings is a core engineering challenge here and we suggest a solution; read about it: www.biorxiv.org/content/10.6...

A quantitative coordinate system for developmental dynamics

Quantitative comparison of morphogenesis across individuals remains a fundamental challenge, as developing embryos vary in shape, orientation and developmental tempo. Moreover, real-time three-dimensi...

biorxiv.org

Nature Cell Biology@natcellbio.nature.com · 4mo ago

☕ @riddhiman.bsky.social et al. use experimentally matched mass-spec and RNA-seq to quantify proteins, phosphosites & transcripts across 4 stages of human & mouse gastruloid development. @jshendure.bsky.social @leastarita.bsky.social @nobuhamazaki.bsky.social @dschweppe.bsky.social bit.ly/4dAYyA1

The proteomic landscape and temporal dynamics of human and mouse gastruloid development - Nature Cell Biology

Garge et al. use experimentally matched mass spectrometry and RNA-sequencing to quantify proteins, phosphosites and transcripts across four key stages of human and mouse gastruloid development.

bit.ly

Nature Cell Biology@natcellbio.nature.com · 4mo ago

☕ @joshifrenster.bsky.social @amartinezarias.bsky.social & co utilize mosaic mouse #gastruloids as a model of cell fitness & competition, identifying a temporal window between primed pluripotency and early gastrulation during which #CellCompetition occurs in mammalian #embryogenesis. bit.ly/4cBmfGK

Mosaic gastruloids reveal a temporal restriction for developmental cell competition - Nature Cell Biology

Frenster et al. utilize mosaic mouse gastruloids as a model of cell fitness and competition, identifying a temporal window between primed pluripotency and early gastrulation during which cell competit...

bit.ly