Please share: The application for Les Houches is open (until Oct 31) fluorescenceleshouches.wordpress.com *This is for you, if you ever wanted to learn all about fluorescence markers & how to apply them for your biological research!* PS: no fees this year, you just need to get there, that's all!

French-German WE-Heraeus-Seminar "Fluorescence markers for advanced microscopy : From photophysics to biology - 2026"

March 15-20 2026, Les Houches, France

fluorescenceleshouches.wordpress.com

Our conference Dinner in the Green House of the Botanical Garden Bonn #SMLMS2025

Attended #SMLMS2025 last week in Bonn, Germany. Many thanks to the organizers @uendesfelder.bsky.social @heilemannlab.bsky.social and Prof. Kubitscheck for introducing #youngSMLMS. That was really mindful of the young researchers including me:). Had a great time in Bonn discussing science.

It was an amazing meeting! Thank you Ulrike (@uendesfelder.bsky.social), Mike (@heilemannlab.bsky.social), Ulrich and all! I’m still under the spell of the scientific insights and the warm, encouraging spirit of this community.💖

Heilemann Lab@heilemannlab.bsky.social · 11mo ago

And we are done --- #SMLMS2025 is over, and it was great. Thank you Uli & Ulrike & all the helpers 👍, and thanks to everyone joining this event 🙏 ... safe travels home & see you soon!

This year’s pre-event symposium #youngSMLMS brings together ECRs in single-molecule microscopy: smlms.org/young-smlms/

#SMLMS2025 has been an absolute pleasure🤩Not to mention the joy of learning about the cool techniques+advances happening in this vibrant community, and meeting some references in the field🥹Thank you very much to the organisers+ #ySMLMS's for the opportunity and the warm,stimulating+energetic event🌟

Heilemann Lab@heilemannlab.bsky.social · 11mo ago

Estibaliz Gomez de Marisca @gomez-mariscal.bsky.social from Lisbon going live and sharing great work on AI-empowered microscopy #SMLMS2025 and accessible AI

And we are done --- #SMLMS2025 is over, and it was great. Thank you Uli & Ulrike & all the helpers 👍, and thanks to everyone joining this event 🙏 ... safe travels home & see you soon!

Helge Ewers @ewerslab.bsky.social takes us on an exciting journey into light-controlled release of single proteins in living cells enabling cool new cell biology such as light-controlled signalling studies #SMLMS2025

🔬Wrapping up #SMLMS2025 @smlm-symposium.bsky.social in Bonn! Thanks to everyone who stopped by our booth! It was great connecting and sharing how our #sdAb-based solutions enhance precision, simplify #Multiplexing, and streamline workflows in #SuperResolution microscopy. See you next year! 🦙

Next on stage is Mahipal Ganji from the IIT Bangalore @ganjilab.bsky.social shares great work on stacking interactions in DNA/RNA and multplexed-DNA PAINT to measure nuclear ultrastructure #SMLMS2025

Thank you @smlm-symposium.bsky.social for the opportunity to present at #SMLMS2025! 🎉 Stay tuned for the upcoming preprint! 📄

Heilemann Lab@heilemannlab.bsky.social · 11mo ago

Sebastian Bauer @sebjbauer.bsky.social from SciLifeLab Stockholm @juliettegriffie.bsky.social reporting #SMLMS2025

Hannah Kim from the Lakadamyali lab at UPenn @melikel.bsky.social reports an analysis pipeline for cell-type specific chromatin analysis #SMLMS2025

Now on stage is Yoav Shechtman from Technion @technion.bsky.social reporting phase mask engineering for 3D SMLM and one-click image reconstruction using a CNN #SMLMS2025

Sebastian Bauer @sebjbauer.bsky.social from SciLifeLab Stockholm @juliettegriffie.bsky.social reporting #SMLMS2025

Next on stage is Remi Galland from @iins-bordeaux.bsky.social reporting a novel platform for in-depth super-resolution imaging #SMLMS2025

Estibaliz Gomez de Marisca @gomez-mariscal.bsky.social from Lisbon going live and sharing great work on AI-empowered microscopy #SMLMS2025 and accessible AI

Anna-Karin Gustavsson @gustavssonlab.bsky.social from Rice University starts off day #3 of #SMLMS2025 with impressive work on whole-cell 3D single-molecule imaging.

#SMLMS2025 delivers outstanding talks again. Glad I could come and enjoy it again.

Heilemann Lab@heilemannlab.bsky.social · 11mo ago

Ever wondered where cargo travels through the central channel of the NPC? Siegfried Musser lab @musser-lab.bsky.social measured that in living cells using MINFLUX. Fascinating data! #SMLMS2025

Next on stage is Jonas Ries @jonasries.bsky.social from Max Perutz Labs in Vienna sharing great work on a MINFLUX simulator that helps in designing experiments 👉🏻 SimuFLUX #SMLMS2025

Ran Huo from TU Delft (Kristin Grußmayer lab) reports very nice work on fast super-resolution microscopy using DNA labels for SOFI #SMLMS2025 www.tudelft.nl/en/faculty-o...

Paul Welzl from the Balzarotti lab @fbzt.bsky.social in Vienna shares impressive imaging data on the 3D shape of mRNA in cells measured with 3D MINFLUX #SMLMS2025 (unpublished data, no images here)

Ever wondered where cargo travels through the central channel of the NPC? Siegfried Musser lab @musser-lab.bsky.social measured that in living cells using MINFLUX. Fascinating data! #SMLMS2025

We are starting the afternoon session with Steffen Sahl from MPI for Multidisciplinary Sciences in Göttingen @mpi-nat.bsky.social sharing exciting MINFLUX data reporting intra-molecular distances and conformations #SMLMS2025

We are so happy to have Zaida Luthey-Schulten here at #SMLMS2025 as a keynote speaker. Zaida is the Director of the NSF Science & Technology Center of Quantitative Cell Biology and Endowed Professor at UIUC. She pioneered 4D modelling of an entire cell integrating experimental data. So inspiring!

Day #2 of #SMLMS2025 is taking off right now and introduced by Uli Kubitscheck👇🏻

First on stage is Maja Klevanski @majaklevanski.bsky.social from Heidelberg University presenting her great work on multiplexed super-resolution imaging of proteins and glycans in tissue #SMLMS2025

Jarno Mäkelä @jmakela.bsky.social from Aalto University shares his great work on single-molecule dynamics of heat shock proteins in E. coli #SMLMS2025 (unpublished work, so no images here)

Johannes Hohlbein @hohlbeinlab.bsky.social from Wageningen presenting his great work on the dynamics of the E. coli replication initiator DnaA #SMLMS2025

👉🏻 check out his recent paper that came out right on time for #SMLMS2025 👍 doi.org/10.1038/s414...

The Escherichia coli replication initiator DnaA is titrated on the chromosome - Nature Communications

A DNA-binding protein, DnaA, regulates the initiation of chromosomal replication in bacteria. Here, Olivi et al. show that the bacterial chromosome sequesters DnaA in a growth rate-dependent manner, s...

doi.org

The next presentation is by Qingru Li from TU Delft reporting really 'cool work' on cryo-4Pi single-molecule localization microscopy #SMLMS2025