Atg1 phosphorylates Atg2 to gate phospholipid flow through a continuous conduit for autophagosome biogenesis

Autophagosome biogenesis requires delivery of millions of phospholipids. In budding yeast, Atg2 and Atg18 couple the ER to the isolation membrane for lipid transport, yet the mechanism is poorly under...

biorxiv.org

bims-placeb 2026-08-02 papers

biomed.news

Phosphorylation of the AP2 µ2 subunit by p70S6 kinase facilitates clathrin-mediated endocytosis

Clathrin-mediated endocytosis (CME) internalizes cell-surface receptors via clathrin-coated invaginations of the plasma membrane. Both clathrin and endocytic cargo are recruited to these sites by the ...

journals.biologists.com

Spatiotemporal control of Atg2 association with the ER during autophagosome formation | PNAS

Autophagy degrades various intracellular components by sequestering them within membrane vesicles called autophagosomes and delivering them to lyso...

pnas.org

Direction of ESCRT-III-dependent membrane bending emerges from bilayer asymmetry

In cells, ESCRT-III is unique in mediating fission of membrane necks from inside, a process called reverse-topology fission. Yet, in vitro, the complex primarily assembles outside membrane necks and m...

biorxiv.org

Rapid removal of nuclear aggregates via proteasome- and VCP-dependent disaggregation

Cells use multiple protein quality control (PQC) mechanisms to counteract the toxic effects of protein aggregation caused by cellular stress, ageing or disease. While it is known that PQC mechanisms d...

biorxiv.org

Lipid-manager autophagy proteins ATG2 and ATG9 regulate extracellular vesicle secretion via amphisome biogenesis and cell lipidome modulation - Nature Communications

Singh et al. reveal that the lipid-handling autophagy proteins ATG9A and ATG2 regulate extracellular vesicle production by shaping cellular lipid composition and controlling whether cargo is degraded ...

nature.com

Lipid-manager autophagy proteins ATG2 and ATG9 regulate extracellular vesicle secretion via amphisome biogenesis and cell lipidome modulation - Nature Communications

Singh et al. reveal that the lipid-handling autophagy proteins ATG9A and ATG2 regulate extracellular vesicle production by shaping cellular lipid composition and controlling whether cargo is degraded ...

doi.org

Electron tomography reveals mitochondrial network and cristae remodelling during cell differentiation in the human placenta - Nature Communications

Mitochondria adapt their structure through fusion and fission. Here the authors couple array and cryo-electron tomography with proteomic analysis to descript changes in the mitochondrial network and c...

nature.com

Conserved and divergent DNA methylation features of extraembryonic tissues between human, mouse and chicken - BMC Genomics

Background The first cell fate decisions in embryogenesis give rise to extraembryonic tissues, such as the yolk sac and placenta, which are essential for embryo survival. In mammals, extraembryonic cell types arising from these first lineage decisions show distinct DNA methylation patterning, including large partially methylated domains, gene body methylation and accumulation across developmental CpG island (CGI) promoters. Analysis of extraembryonic membranes beyond mammalian species has been limited. Using bisulphite-seq and RNA-seq, we comparatively define these epigenetic characteristics of extraembryonic tissues in both mouse and human. We then tested whether these features are conserved in the chicken extraembryonic membranes (EEMs) including the yolk sac, chorion and chorioallantoic membrane (CAM). Results Transcriptomic analysis revealed that embryonic day (E)4 yolk sac/chorion and E14 CAM express similar marker genes to the mouse chorionic trophoblast, supporting that the chicken chorionic ectoderm shares a ‘trophoblast-like’ cell identity. We find that partially methylated domains and transcription-linked gene body methylation patterning are highly conserved between mouse and human proliferative multipotent trophoblast cells but were not evident in the chicken EEMs. However, we find that methylation of CGIs associated with developmental genes was a conserved feature of mouse, human and chicken EEMs. Conclusions These findings support that features of the extraembryonic DNA methylation landscape have distinct underlying mechanisms. We find that only one feature is conserved across extant amniotes, suggesting distinct evolutionary pressures are associated with different features of EEM epigenetic landscape.

tinyurl.com

Electron tomography reveals mitochondrial network and cristae remodelling during cell differentiation in the human placenta - Nature Communications

Mitochondria adapt their structure through fusion and fission. Here the authors couple array and cryo-electron tomography with proteomic analysis to descript changes in the mitochondrial network and c...

nature.com

Rapid optogenetic blockade of autophagy reveals that nuclear pore complex proteins are robust autophagy substrates

Autophagy, a conserved recycling process, manages intracellular quality control to mitigate stress. To determine the rapid effects of autophagy perturbation, we developed the first optogenetic tool to...

biorxiv.org

ATG-9-Induced Lysosomal Membrane Permeabilization and Cell Death in a Caenorhabditis elegans model of Mucolipidosis type IV

Mucolipidosis type IV is a lysosomal storage disease that is characterized by delayed psychomotor development and retinal degeneration due to cell death, in addition to other symptoms that are due to ...

biorxiv.org

Placental Cell Biology Group | NIEHS | Durham, NC

Our lab studies how the placenta develops and functions, using state-of-the-art cell biology approaches. This enables us to explore interventions to diagnose and treat complex pregnancy disorders of p...

guardialab.com

How the U.S. is losing ground to China in university research

Two researchers — one in Massachusetts and one in Shanghai — hoped for the same breakthrough: a gene therapy for deaf children. New Chinese investment in science propelled the one who got there first.

n.pr

Justin Taraska, Ph.D. | Principal Investigators | NIH Intramural Research Program

irp.nih.gov