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Daniel L. Barber, PhD

@danbarberphd.bsky.social

Immunologist. Major focus is on T cell responses to Mycobacterium tuberculosis infection.

19 Posts2347 Follower279 Folgt
Daniel L. Barber, PhD@danbarberphd.bsky.social · 3mo ago

So we blocked IFNγ in Mtb infected macaques and….the animals were fine. Turns out mice overestimate the importance of IFNγ in TB because IFNγ drives iNOS in mice but not macaques or humans. This means we don’t actually know how T cells suppress Mtb. rdcu.be/ff9GB #tuberculosis #immunology

IFNγ blockade in Mycobacterium tuberculosis infected macaques alters the granuloma environment but not bacterial control

Nature Communications - Here, the authors show that IFNγ blockade does not impair resistance to Mycobacterium tuberculosis infection in macaques, opposed to what has been observed in mice, due...

rdcu.be

Daniel L. Barber, PhD@danbarberphd.bsky.social · 5mo ago

In this new paper by @nelson-lab.bsky.social, we found that pre-exposure of NHP to glucocorticoids impairs control of SARS-CoV-2 and is associated with reduced pDC, eosinophil and γδT cell responses. journals.plos.org/plosone/arti...

Glucocorticoids suppress early lung inflammation and impair control of SARS-CoV-2 in non-human primates

In severe cases of COVID-19, glucocorticoid treatment improves clinical outcomes. However, in non-hospitalized patients, glucocorticoids have limited benefit and may impair viral clearance. Here, we u...

journals.plos.org

Daniel L. Barber, PhD@danbarberphd.bsky.social · 7mo ago

Important paper by Björn Corleis. For decades a central tenet of #tuberculosis immunology has that IFNγ-driven iNOS expression in macrophages is the primary mechanism of bacterial control. Turns out this is really only in mice and maybe cattle. It’s not true in most species including humans an NHP.

bioRxiv Immunology@biorxiv-immuno.bsky.social · 7mo ago

Species-specific iNOS expression distinguishes epithelial and myeloid IFNγ responses in tuberculosis https://www.biorxiv.org/content/10.64898/2026.01.11.697682v1

Daniel L. Barber, PhD@danbarberphd.bsky.social · 8mo ago

What we’ve got here is failure to communicate - some macrophages you just can’t reach! Multiple features of granulomas limit effective T cell:MΦ interactions, posing a major barrier for vaccine-mediated protection. Check out our new review! onlinelibrary.wiley.com/doi/10.1111/...

<em>Journal of Internal Medicine</em> | Wiley Online Library

Tuberculosis (TB) remains a leading infectious cause of morbidity and mortality, and the development of a new, highly effective vaccine would have a tremendous beneficial impact on global health. Alt....

onlinelibrary.wiley.com

Daniel L. Barber, PhD@danbarberphd.bsky.social · 11mo ago

We are looking to hire post-docs to work on T cell responses to M. tuberculosis infection using murine and NHP models. If you know T cells and wanna work on the top global infectious killer check us out. BSL3 experience not required. The NIH intramural program in Bethesda MD is an amazing place!

Daniel L. Barber, PhD@danbarberphd.bsky.social · last yr.

We are looking for post-docs interested in studying T cell responses to M. tuberculosis infection…especially basic T cell immunologists who are interested in learning to work with BSL3 pathogens. The NIH intramural program is an amazing place to train!

Paul Baker@pauljbaker.bsky.social · 2y ago

More details in this 🧵 from my powerhouse PI @mayerbarber.bsky.social whose foresight and guidance got us to this fantastic manuscript!

Kat Mayer-Barber,PhD@mayerbarber.bsky.social · 2y ago

Best #Nikolaus 🎅! Our paper on how the 🫁 microenvironment can shape #innate immunity against #viruses is out @sciimmunology.bsky.social This was a herculean effort brilliantly led by @pauljbaker.bsky.social who singlehandedly established the model in the lab during the pandemic. 🧪 #Immunosky 1/9

Graphical summary of our paper. In mice, prior lower airway exposure to diverse inflammatory stimuli, including chronic bacterial infections such as M. tuberculosis, acute bacterial infections such as pulmonary S. aureus, viral infections such as Influenza A, type-II allergic responses such as the OVA-Alum model, activation of pulmonary TLR9 by CpG or pulmonary TLR1/2 by Pam3CSK4 leads to reduced viral burden upon subsequent infection with SARS-CoV-2 (SCV2). (2) This SCV2 restriction occurs prior to induction of SCV2-specific adaptive immune responses and is mediated through innate immune responses, including the induction of IFN-I, TNFα and IL-1 and sustained changes to the TRM (Tissue resident macrophage) cellular compartment and the pulmonary epithelium. (3) Innate cytokine and TLR signaling to both recruited immune cells and the pulmonary epithelium creates a microenvironment in the lung that limits early replication of SCV2. IFN-I signaling to pulmonary ECs (epithelial cells) increases expression of interferon-stimulated genes, that likely cell-intrinsically limit viral replication. TNF- or IL-1 suppress SCV2 independently of IFN-I signaling. TNF acts exclusively through radio-resistant cell types such as the lung epithelium, whereas IL-1 affords control both direct and indirectly, through either stromal and hematopoietic cell types, to restrict overall early SCV2 burden.
Paul Baker@pauljbaker.bsky.social · 2y ago

Excited to share this paper from my post-doc at @niaidnews.bsky.social out now in Science Immunology! We show that recent or ongoing 🫁 infections or other pulmonary environmental exposures at the time of infection with SARSCoV2 can vastly influence early replication of the virus in mice.

The inflammatory microenvironment of the lung at the time of infection governs innate control of SARS-CoV-2 replication

Recent or ongoing respiratory inflammation in the lung regulates antiviral immune responses to SARS-CoV-2.

science.org

Kat Mayer-Barber,PhD@mayerbarber.bsky.social · 2y ago

🙏 to @niaidnews.bsky.social, first author @pauljbaker.bsky.social and my amazing lab team, our collaborators @kerryhilligan.bsky.social @danbarberphd.bsky.social 🙏to editor extraordinaire @hannah-isles.bsky.social and the reviewers who guided us along the way 9/9 www.science.org/doi/10.1126/...

The inflammatory microenvironment of the lung at the time of infection governs innate control of SARS-CoV-2 replication

Recent or ongoing respiratory inflammation in the lung regulates antiviral immune responses to SARS-CoV-2.

science.org

Kat Mayer-Barber,PhD@mayerbarber.bsky.social · 2y ago

Excited for my first post here to be our new #preprint. 👇🏻check it out below … the recent pulmonary exposure history matters for limiting SARS-CoV-2 viral titers in the 🫁”tweetorial” to follow later

bioRxiv Immunology@biorxiv-immuno.bsky.social · 2y ago

The inflammatory microenvironment of the lung at the time of infection governs innate control of SARS-CoV-2 replication https://www.biorxiv.org/content/10.1101/2024.03.27.586885v1