Calling all Developmental Biologists! We're accepting self-funded PhD students to work on Wnt and Notch signalling using Gastruloids! Spread the word! www.liverpool.ac.uk/courses/mole...

To find out more about this work and the people behind it, we spoke to first author Tina Balayo, and to co-corresponding authors André Dias @dias-andre.bsky.social and David Turner @gastruloids.bsky.social: journals.biologists.com/dev/article/...

Delighted that this article is now out journals.biologists.com/dmm/article/... Great team work with Dharani Hapangama, Chris Hill and first author Dimas Rahmana from @livuni-ilcams.bsky.social @livunihls.bsky.social With generous funding from the Indonesian government.

Compound design of a patient-derived 3D cell culture system modelling early peritoneal endometriosis

Summary: Endometriosis cannot be accurately modelled in laboratory animals. Our in vitro peritoneal endometriosis model uses patient-derived cells to accelerate understanding of disease onset processe...

journals.biologists.com

Listening to David Lammy on @BBCr4today attempting to justify abolishing trial by jury, it is increasingly and terrifyingly clear that he simply does not understand the issues.

As set out in this thread, the causes of the court backlog are chronic lack of funding and absurd inefficiency. The government has made no proposals to address either. Their answer is to remove juries. Rather than fix the leaking roof, their solution is to burn the house down.

The Secret Barrister@barristersecret.bsky.social · 8mo ago

Here is a list of reasons why some of my hearings and trials this year have been delayed and kicked off into the long grass, stuck in our record court backlog. Serious allegations which will now be tried *years* after the event. 🧵👇

Now out in @dev-journal.bsky.social Lots of new results! Check it out!! journals.biologists.com/dev/article/...

N2B27 media formulations influence gastruloid development

Highlighted Article: This work explores the cellular and molecular sensitivity of the mouse gastruloid model system to variations in N2B27 media formulations, highlighting the need for careful media s...

journals.biologists.com

André Dias@dias-andre.bsky.social · last yr.

Fun collaboration! All the nitty-gritty about N2B27 and gastruloids. Kudos to all the authors, particularly Tina Balayo (¡que va de primero!)

There's increasing evidence that in organoid/embryoids conditions of cells at start of experiments is crucial/ determines outcome. Much to be learnt still. Taking advantage of mouse #gastruloids, two groups explore this here. Important work to calibrate the field www.biorxiv.org/content/10.1...

Now out in @dev-journal.bsky.social journals.biologists.com/dev/article/...

N2B27 media formulations influence gastruloid development

Highlighted Article: This work explores the cellular and molecular sensitivity of the mouse gastruloid model system to variations in N2B27 media formulations, highlighting the need for careful media s...

journals.biologists.com

I do like the @dev-journal.bsky.social "Research Report" format for shortish papers This one is valuable. "Is it the N2B27?" is the stem cell biologist version of "Is it the magnesium?" Congrats Tina Balayo @dias-andre.bsky.social David Turner & all authors journals.biologists.com/dev/article/...

N2B27 media formulations influence gastruloid development

Highlighted Article: This work explores the cellular and molecular sensitivity of the mouse gastruloid model system to variations in N2B27 media formulations, highlighting the need for careful media s...

journals.biologists.com

Like Wnt signalling? How about Notch? How about both together (Wntch!)... in gastruloids? Come and and apply for a PhD studentship with us in Liverpool! Applications open 30th October! tinyurl.com/3bpx3y2b

MRC DiMeN Doctoral Training Partnership: How does Wnt and Notch (Wntch) signalling coordinate cell fate assignment during gastrulation? at University of Liverpool on FindAPhD.com

PhD Project - MRC DiMeN Doctoral Training Partnership: How does Wnt and Notch (Wntch) signalling coordinate cell fate assignment during gastrulation? at University of Liverpool, listed on FindAPhD.com

tinyurl.com

This year's Crick @crick.ac.uk PhD programme is open for applications - £27,715 tax-free student stipend per year for 4 years - Tuition fees paid - Student visa fee & health surcharges covered Deadline 5 Nov We have a project available. For all the details visit: www.crick.ac.uk/careers-stud...

New insights directly link WT1 and smooth muscle cell lineages Read this Research Highlight showcasing work from Suad Alsukari, Huei Teng Ng, @gastruloids.bsky.social, Bettina Wilm @bwilm.bsky.social and colleagues: journals.biologists.com/dev/article/...

Very happy to announce that this article is now published @dev-journal.bsky.social @biologists.bsky.social journals.biologists.com/dev/article/... With Suad Alsukari and Huei Teng Ng and @gastruloids.bsky.social

Dynamic WT1 expression during gastrulation specifies peritoneal smooth muscle fate independently of mesothelial fate

Highlighted Article: Genetic lineage tracing and expression analyses reveal insights into the developmental lineage of smooth muscle cells in the intestine and mesentery, specified by WT1 expression d...

journals.biologists.com

An englishman, down and out in Paris…..trying to read the news and laughing....at a french joke? @gastruloids.bsky.social‬

Look at this amount of work that has gone into the analysis of these beautiful (yes, we are biased) images of rods and rings. Now you can automatically classify these structures which is rather tedious to do manually. 🔬📷🖼️💻📄 bmcbiol.biomedcentral.com/articles/10....

Domain-specific AI segmentation of IMPDH2 rod/ring structures in mouse embryonic stem cells - BMC Biology

Background Inosine monophosphate dehydrogenase 2 (IMPDH2) is an enzyme that catalyses the rate-limiting step of guanine nucleotides. In mouse embryonic stem cells (ESCs), IMPDH2 forms large multi-prot...

bmcbiol.biomedcentral.com

I cannot help but think of these rod-rings as oh so pretty. amazing work by some of our @cci-liv.bsky.social users.

CCI Uni of Liverpool@cci-liv.bsky.social · last yr.

Look at this amount of work that has gone into the analysis of these beautiful (yes, we are biased) images of rods and rings. Now you can automatically classify these structures which is rather tedious to do manually. 🔬📷🖼️💻📄 bmcbiol.biomedcentral.com/articles/10....

Great preprint out from the MicroAge team in @livuni-ilcams.bsky.social! www.biorxiv.org/content/10.1...

Excellent work supporting #modularity of the mammalian #embryo, a role for #hypoxia & how development exploits these features. It shows what we can learn from assemblies of #gastruloids @ spatio-temporal coordination of the modules in AP organization. Putting modules together, extremely informative

Aydan Bulut-Karslioglu@bulutkarslioglu.bsky.social · last yr.

⚠️ I am really excited to share the work of Anastasios Balaskas, an excellent PhD candidate in the lab, with the wider world. Tasos made a significant advance: generating a stem cell-based embryo model that contains both posterior and anterior neural tissues of the late-stage gastrulating embryo.

The modularity of the mammalian #embryo in display. Controlled assembly of classic #gastruloids and uncommitted precursors in #hypoxia, patterns the AP axis www.biorxiv.org/content/10.1... Beautiful & insightful from @bulutkarslioglu.bsky.social‬ group. Building models to learn and learning.

This was a fabulous collaboration! Thanks @gastruloids.bsky.social and to Suad and Huei who worked incredibly hard to make it happen!

Great to see our article published in @dev-journal.bsky.social! Wonderfull collaboration with @bwilm.bsky.social to understand how Wt1 guides cell fate in the early gastrulating embryo! journals.biologists.com/dev/article/...

Also, an interesting reflection from Bob Edwards on the contrast between Research Institutes and Universities. Having spent my academic life in the latter, I can relate. From "A matter of life" a first hand account of the path to IVF that I suggest as a read for those interested in human #DevBio

Great to see our article published in @dev-journal.bsky.social! Wonderfull collaboration with @bwilm.bsky.social to understand how Wt1 guides cell fate in the early gastrulating embryo! journals.biologists.com/dev/article/...

Dynamic WT1 expression during gastrulation specifies peritoneal smooth muscle fate independently from mesothelial fate

The Wilms’ Tumour protein (WT1) was previously linked to the mesothelial and vascular smooth muscle cell (vSMC) lineage in the mouse intestine, with evidence suggesting that intestinal vSMCs arise fro...

journals.biologists.com

We previously showed that DNA Typewriter can record high-resolution lineage trees in cultured cells (HEK293T). www.nature.com/articles/s41... But could we use it to study how clonal memory shapes cell fate decisions during development? (2/13)

A time-resolved, multi-symbol molecular recorder via sequential genome editing - Nature

A DNA memory device, DNA Typewriter, uses sequential prime editing to record the order of multiple cellular events.

nature.com

New preprint with the Shendure lab @jshendure.bsky.social ! We apply the DNA Typewriter lineage recorder to mouse 3D gastruloids, a powerful model of early mammalian development. Led by Sam Regalado and CX Qiu @cxqiu.bsky.social : www.biorxiv.org/content/10.1... (1/13)

Lineage recording in monoclonal gastruloids reveals heritable modes of early development

Mammalian stem cells possess a remarkable capacity for self-organization, a property that underlies increasingly sophisticated in vitro models of early development. However, even under carefully contr...

biorxiv.org

We've just updated our manuscript to a more 'publishable' version! www.biorxiv.org/content/10.1... New details about the role of Wnt in suppressing neural differentiation and fostering a posterior epiblast state, and the temporal effect of Nodal signalling in driving mesendodermal fates

André Dias@dias-andre.bsky.social · 2y ago

www.biorxiv.org/content/10.1... Here we explore the role of #Wnt and #Nodal signalling during mammalian gastrulation using #gastruloids. Building on the work of many labs, we propose that antagonistic signalling-dependent #modularity in the PS establishes the early mammalian body plan.

Our new paper just arrived! Fun collaborative effort led by Sam Ball and Meagan Hennessy to develop an AI tool to segment and classify #IMPDH2 rod-rings in mouse ESCs doi.org/10.1186/s129...

Domain-specific AI segmentation of IMPDH2 rod/ring structures in mouse embryonic stem cells - BMC Biology

Background Inosine monophosphate dehydrogenase 2 (IMPDH2) is an enzyme that catalyses the rate-limiting step of guanine nucleotides. In mouse embryonic stem cells (ESCs), IMPDH2 forms large multi-prot...

doi.org

Great fun at last night's Gala Dinner at the stunning St Georges Hall. #biologists100

Right after the spanish edition of #TheMasterBuilder comes the Portuguese one, thanks to www.temasedebates.pt Have a look for a cell’s eye view of Biology in contrast with the widespread gene’s eye. #CellsRUs #NotInTheGenes

Wonderful evening in #Barcelona w/ friends sharing memories, science and hope in these troubled times.

In the wake of Barcelona #Gastruloid meeting, we can say w/ some confidence that this is a system w/ potential in several areas of biomedical research: models, rather than replicas, are useful &also teach. For details linkinghub.elsevier.com/retrieve/pii... onlinelibrary.wiley.com/doi/10.1002/...