This #FluorescenceFriday we have an Endosome sorting itself out! 37min of PI(3)P (green) positive sorting endosome with fluorescent Transferrin in Magenta. The sorting of Transferrin is tightly interlinked with PI(3)P intensity. Soon we will publish how cargo sorting controls PI(3)P levels!

Hopefully the reputational damage of being on the front line international news will make @healthcare.nikon.com reconsider how they deal with this disgrace to the microscopy community and their inaptitude to adhere to their own rules www.bbc.co.uk/news/article...

Tiny image sparks big backlash in Nikon photo contest

Dr Ning Xu denies he broke the rules of Nikon's annual Small World In Motion contest.

bbc.co.uk

It’s amazing how much some mathematical modelling can add to a cell biology problem. When I hired my first postdoc two years ago I took a bit of a gamble by hiring someone with computational background and little wet lab experience and I think it’s was the best decision ever.

Opportunity in @cambiochem.bsky.social for those looking to make their first step to independence: www.bioc.cam.ac.uk/research/ind...

Finally reached the point where I completely hate the paper I have been writing and working on for the last three years! This means we must be getting close....

I know I am probably very late to realising this but I really don’t like the layout of @embojournal.org website since they joined with springer. Makes it look just like another run down the mill Springer journal and has lost its own identity

Starting to write up the first mechanistic paper from my lab about regulation of endosomal PI(3)P. We see retromer tubules (Vps35) budding from PI(3)P positive sorting endosomes with Actin at the neck. When PI(3)P homeostasis is perturbed this organisation breaks down and cells cant recycle cargo.

Nothing better for procrastination than going through some live cell imaging of Endosomes... Today we have the fusion of two equally sized PI(3)P positive endosomes. This is at 5s framerate, wish I had taken it at higher temporal resolution but still super cool to see

How do Endosomes dance? Looks like a waltz? Or maybe the more of a cèilidh? Cells expressing the PI(3)P probe imaged every 5s for ~15min. Currently preparing a really exciting paper about the regulation of endosomal PI(3)P homeostasis. Stay tuned!

Starting to put together an exciting paper about endosomal membrane identity and PowerPoint's AI knows exactly what I am up to: "A collage of different spots". Sums up this whole paper pretty well 😅

Great resource and evaluation on everything to do with preprints in the life sciences! Preprints have been transformational and there is a lot of good work being done at @ripplingideas.bsky.social !

Rippling Ideas@ripplingideas.bsky.social · 8mo ago

Our report on the current state of preprinting in the life sciences is out! Read it here: zenodo.org/records/1... & watch our video on the key data: youtu.be/G_mmmkuBua8 #preprints #scicomm #academicsky

Imaging phosphoinositides during mitosis ! Cells stained against PI(4,5)P2 using recombinant biosensors (tinyurl.com/447fabz8) PI(4,5)P2 is up regulated for cortex-membrane attachment. Also notice the membranes being shed and taken up by non-mitotic bystanders. #blebbisomes @mag2art.bsky.social?

We're hiring! Enjoyed @jackholcombe.bsky.social PhD Prize talk @edrc2025.bsky.social @fly-eds.bsky.social? We're recruiting a #postdoc to explore how cell biology is patterned in the #Drosophila renal system. Come join us for live-imaging, molecular cell biology and omics! Deadline 25th January.

Jack Holcombe@jackholcombe.bsky.social · last yr.

Such an amazing experience sharing science with a wonderful community! Thanks so much 🧬🔬@edrc2025.bsky.social @fly-eds.bsky.social

Early bird deadline in 10 days!! This is going to be amazing!

The Company of Biologists@biologists.bsky.social · 9mo ago

Early-bird deadline to join @jcellsci.bsky.social at their meeting on Imaging Cell Dynamics in Spain in May is Friday 16 January. Apply to attend at: biologists.com/meetings/jcs... #JCSimaging

Two days left to apply for a PhD position in plant development with me, Sam Amsbury and Andrew Fleming!!

Lisa Smith@plantylisasmith.bsky.social · 11mo ago

Interested in plant development and/or cell walls? We (Sam Amsbury, Andrew Fleming and I) have three PhD positions at the University of Sheffield open for applications until 7th January. We offer a friendly, jointly-run lab, great facilities, an affordable city and proximity to the Peak District!

While this does not solve the underlying issues with for-profit entities exploiting the free labour of academics, it does make my life easier in the short term. At the moment funders require me to publish open access while refusing to pay for the APC (a bit of a catch22). So this will help with that

Jisc@jisc.bsky.social · 10mo ago

We've secured landmark agreements with all five major academic publishers, giving UK universities read and publish access to 11,000+ journals and breaking pricing links to article volume.

#membrane seminars are back this THURSDAY for a super session with @dr-downes.bsky.social from the @giuliazanetti.bsky.social lab and Xin Zhou from the Ikonen lab! @felixmendu.bsky.social @ishier.bsky.social

This is making the rounds and people are be worried about fake data being published. I don’t see the point. All data can already be faked. All graphs and numerical data can freely be made up. Controls and samples can be switched. If we can trust scientists and institutions we might as well stop.

Pedro Beltrao@pedrobeltrao.bsky.social · 11mo ago

I tried an even harder example on Gemini Pro image generation and this is quite scary/amazing. I asked for a microscopy image of around 20 HeLa cells, GFP tagged 20% nuclear, 10% membrane, +1 nuclear staining, + overlap. Image below and prompt in the following post.

Really glad to show our recent work using recombinant phosphoinositide probes to investigate membrane identity changes during endosomal trafficking. Should be a great mini symposium to highlight the power of fluorescent microscopy techniques for cell biology!

FocalPlane @focalplane.bsky.social · last yr.

Join us this Thursday for our mini-symposium with the membrane trafficking webinar series Featuring talks from @nickader.bsky.social, @maiblab.bsky.social and Mónica Quiñones-Frías, and a keynote lecture from Gillian Griffiths @griffithslab.bsky.social. focalplane.biologists.com/2025/09/16/f...

📣Paper alert! Interested in faster and brighter protein labeling than before? 👀 @steffi-k.bsky.social & other colleagues have developed an improved tool for labeling proteins with synthetic fluorophores for bioimaging. Also works in STED microscopy 🤩🔬 Congratulations!🥳 www.nature.com/articles/s41...

SNAP-tag2 for faster and brighter protein labeling - Nature Chemical Biology

SNAP-tag is a widespread tool for labeling protein for bioimaging. Now, Kühn et al. report SNAP-tag2 with increased labeling kinetics and brightness, which translates into a better performance in live...

nature.com

Shit.

In all my interactions with @jacoates.bsky.social I can vouch for his sincere commitment and drive towards open science and engagement. Moving on from his role with ASAPbio will be a great opportunity for whomever he will work with next!

Jonny Coates@jacoates.bsky.social · last yr.

I’m urgently seeking a new role. If you’re in the preprint/open science/academic culture space then I am the perfect person to join your team. Here's some reasons why 🧵 1/9

Choose Science. Choose Europe. A new Marie Skłodowska-Curie Actions Postdoctoral Fellowships 2025 call is now open. With a budget of €404.3 million, it will support around 1,650 researchers from Europe and beyond. Apply by 10 September → europa.eu/!fBTMgF

Looking for a student or postdoc! Want to combine evolutionary analysis + structural modeling with in vitro biochemistry & fluorescence microscopy? Help us uncover new protein-protein interactions — from in silico to in vitro. Join us at @istaresearch.bsky.social 👉 looselab.org 🧬💡🔬🎡

If using Bloomigton #Drosophila Stock Center stocks, pls. acknowledge them & their NIH funding (P40 OD018537). Papers listing this no. are being harvested as evidence. We massively depend on the @bdsc.bsky.social & they need our support in these dire times! @flybase.bsky.social @fly-eds.bsky.social

Pls re-skeet! There's a postdoc job available to join us in Bristol! We're looking for a molecular cell biologist to help us investigate plasma membrane proteostasis in neurons. Apply here by 23 April: www.jobs.ac.uk/job/DMO097/r.... If interested, do contact me and check out grieve-lab.com! Thanks!

Only 10 days left to apply! We are looking for a passionate protein biochemist for the reconstitution of lipid kinases. We have long term funding and you will be trained in the use of supported lipid bilayers. If you are interested just get in touch or apply directly!

Hannes Maib@maiblab.bsky.social · 2y ago

🚨Long term postdoc position available on the reconstitution of lipid kinases!🚨 Do you have a passion for protein reconstitution and lipid signalling? You will reconstitute lipid kinases from eukaryotic expression systems to determine their kinetic regulation. www.jobs.ac.uk/job/DLW981/r...

First time playing around with single molecule STORM imaging of PI3P on endosomes using recombinant biosensors. This was great fun with our new fellow Ruby Peters and I see lots of potential for this in the future!

🚨Long term postdoc position available on the reconstitution of lipid kinases!🚨 Do you have a passion for protein reconstitution and lipid signalling? You will reconstitute lipid kinases from eukaryotic expression systems to determine their kinetic regulation. www.jobs.ac.uk/job/DLW981/r...

Research Associate in Reconstitution of Lipid Kinases at University of Sheffield

An opportunity for an academic position as a Research Associate in Reconstitution of Lipid Kinases is available, as advertised on jobs.ac.uk. Apply now and explore other academic job openings.

jobs.ac.uk

The open science community must lead by example in defending science. We are ideally positioned to combat the efforts by Trump, Musk & others that are anti-science & anti-inclusion. Time to practice what we preach! #academicsky #openscience #metasci #ScholComm

Science is Under Siege: The Open Science Community Must Act and Lead by Example

Worldwide, science is under attack by anti-scientific movements. The new US administration has thrown the entire academy into chaos, threatening global health. This article is a call for the open scie...

upstream.force11.org