Sequence-based prediction of intermolecular interactions driven by disordered regions

Intrinsically disordered regions (IDRs) in proteins play essential roles in cellular function. A growing body of work has shown that IDRs often interact with partners in a manner that does not depend ...

science.org

Large-scale control over collective cell migration using light-activated epidermal growth factor receptors

Programmable control over tissue movement is a fundamental challenge for tissue engineering and wound healing. Suh, Thornton, et al. discovered that a light-controlled EGF receptor controls long-range...

cell.com

Protein codes promote selective subcellular compartmentalization

Cells have evolved mechanisms to distribute ~10 billion protein molecules to subcellular compartments where diverse proteins involved in shared functions must assemble. Here, we demonstrate that prote...

science.org

SEED-Selection enables high-efficiency enrichment of primary T cells edited at multiple loci - Nature Biotechnology

Primary T cells with multiple genetic modifications are rapidly isolated by negative selection.

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Multiplex generation and single-cell analysis of structural variants in mammalian genomes

Studying the functional consequences of structural variants (SVs) in mammalian genomes is challenging because (i) SVs arise much less commonly than single-nucleotide variants or small indels and (ii) ...

science.org

A map of the rubisco biochemical landscape - Nature

A massively parallel assay developed to map the essential photosynthetic enzyme rubisco showed that non-trivial biochemical changes and improvements in CO2 affinity are possible, signposting further e...

nature.com

Multiscale footprints reveal the organization of cis-regulatory elements - Nature

We developed PRINT, a computational method that identifies footprints of DNA–protein interactions from bulk and single-cell chromatin accessibility data across multiple scales of protein size.

nature.com

Hashing the message with cells - Nature Chemical Biology

The programming of multicellular processes for biological computation requires increasingly complex genetic circuit design. Through automated circuit design, it is now possible to systematically break...

nature.com

Molecular determinants of condensate composition

Biomolecular condensates are non-stochiometric assemblies that organize cellular matter in space and time. Despite much work, many questions remain regarding what determines the recruitment and exclus...

cell.com

Orthogonalized human protease control of secreted signals

Nature Chemical Biology - Engineering of a human-derived protease controlled exogenously by its FDA-approved inhibitor enables control over cytokine activity in cell-based therapies with reduced...

rdcu.be

A foundation model of transcription across human cell types - Nature

A foundation model learns transcriptional regulatory syntax from chromatin accessibility and sequence data across a range of cell types to predict gene expression and transcription factor interactions...

nature.com

Predicting RNA-seq coverage from DNA sequence as a unifying model of gene regulation - Nature Genetics

Borzoi adapts the Enformer sequence-to-expression model to directly predict RNA-seq coverage, enabling the in-silico analysis of variant effects across multiple layers of gene regulation.

nature.com

Engineering synthetic phosphorylation signaling networks in human cells

Protein phosphorylation signaling networks have a central role in how cells sense and respond to their environment. We engineered artificial phosphorylation networks in which reversible enzymatic phos...

science.org