Phenomenal #glycotime by Meg Lunn-Halbert, Joel Allen and colleagues demonstrates through de novo protein design how steric hindrance controls N-glycan maturation ⬇️ doi.org/10.64898/202... Protein design with RFdiffusionAA coupled with glycosylation with GlycoShape 🧪

Refreshed ReGlyco Filter in bioRxiv 🧪(doi.org/10.64898/202...) in view of the upcoming Protein Design Competition on Monday 28/09 🎉🔥 proteinbase.com/competitions... Each week a new target and if it's a glycoprotein, glycoshape.org and ReGlyco Filter can help screen and optimise your binder design

Fantastic opportunity to talk, listen and discuss the future of #glycotime 🧪 with ECRs and PhD students at the upcoming GRS Glycobiology 2027 organised by the über talented @j-a-n-alexander.bsky.social and @lornamilne.bsky.social who put together a sensational programme. Register asap!! ⬇️

🚨 Want to be part of top #glycotime on the seaside in the California sunshine? Register to the 2027 GRC Glycobiology ⬇️ Stacy and Lance put togteher up one of the most exciting programmes I have ever seen with phenomenal keynotes! We have 32 abstracts to select, so the next speaker could be YOU! 😎

🚨 New version of GlycoShape is now live at glycoshape.org 🥳 Powered by crabWURCS everything runs on your browser and while you save all you ReGlyco work and go back to it whenever you like 😎 Among other new things: new slick drawer, much easier to use, new slick SNFG representations and new layout

I will be in Geneva at #ECCB2026 for a super #glycotime workshop in glyco-bioinformatics hosted by @glycoexpasy.bsky.social to present a hands-on guide to the new layout and features of @glycoshape.org If you want to hear all about it, just sign up! ⬇️ eccb2026.org/communities-... online and free

We then took a deeper dive into our EpCAM data to look at what integrating bottom-up glycopeptide and intact glycoproteoform measurements can get us, and @emmajays.bsky.social helped us use @glycoshape.org to model how a glycoproteoform with two occupied N-glycosites might look like.

Want to know more about #glycotime resources in bioinformatics and/or build some glycoproteins with GlycoShape? Join us online on Sept 4th for fabulous hands-ON workshops organised by @glycoexpasy.bsky.social 😎🥳🧪 Online registration is FREE (Price: 0 CHF) here, eccb2026.org/communities-...

@glycoexpasy.bsky.social · 2mo ago

Attending @eccb-europe.bsky.social in Geneva this year? Then, you can join our workshop! Programme and registration is here: eccb2026.org/communities-... it's free 🙂 but hurry!

⬇️ #glycotime 🧪

Elisa Fadda@elisafadda.bsky.social · 3mo ago

Isn't it amazing when a fresh new preprint is out on Friday? 😎🥳 Check out the fabulous work by @beatricetropea.bsky.social who gives us a clear mechanistic explanation behind the 'peculiar' structure of the LLO donor preferentially chosen by eukaryotic OST 🤓😍 doi.org/10.64898/202...

The fully glucosylated LLO structure is the preferred substrate of eukaryotic OST, especially engineered to enhance N-glycosylation efficiency; but how does that work? and why do we need all those glucoses? @beatricetropea.bsky.social answers all these Q in a new preprint ⬇️ doi.org/10.64898/202...

How the terminal glucoside of the N-glycan donor affects the catalytic efficiency of the eukaryotic oligosaccharyltransferase

The eukaryotic oligosaccharyltransferase (OST) is the enzyme responsible for initiating N-glycosylation of secreted proteins by transferring a pre-assembled lipid-linked oligosaccharide (LLO) donor to...

doi.org

As a fun educational/inspirational #glycotime we decided to curate a Blog on glycoshape.org, you can find it under Resources or here glycoshape.org/blog You can *sign up* to get an email when we post a new entry The latest is E04:Let's talk about glycan binding to viral (glyco)proteins 🧪

Un-crystallisable glyco-complexes anyone? New work led by John Klassen's lab introduces antibody masking-native mass spectrometry (AM-nMS) a spatially resolved assay that allowed us to map glycan binding sites across the SARS-CoV-2 RBD. The results match our MDs! 😜 All details glycoshape.org/blog

glycoshape.org has now a blog where we discuss (yep you got it) glycans, glycoproteins and fun stuff! Season 1 is out with: Ep01: Why should we care about glycan heterogeneity? Ep02: How can we include glycan heterogeneity in a 3D framework? Ep03: Molecular precision glycoscience: Enter Siglecs

Next time you hear 'glycans are just a fancy protein decoration' 😱 show them this fabulous work led by Katrine Schjoldager and team at the CGR 🇩🇰 with us 🇬🇧 showing how The ligand preference of LRP1 is regulated by O-glycans Out now www.science.org/doi/10.1126/... 🧵1/10 ⬇️

The ligand preference of LRP1 is regulated by O-glycans

GALNT11-mediated O-glycans modulate LRP1 uptake of tau and Aβ, unveiling glycan-driven mechanisms in neurodegenerative pathways.

science.org

Glycoheterogeneity is an aspects of glycobiology that most would class into a category between difficult and intolerable, but @benschulz.bsky.social and us love it! So we wrote a whole review in Current Opinion in Structural Biology on it 🧵1/8 ⬇️ doi.org/10.1016/j.sb...

🚨 #glycotime seminar alert! June 22nd, 2026 4pm JST (8am BST) @kiyokof.bsky.social will present the GlyTouCan v.4 in the next GlySpace Alliance Seminar event 🔥 If you use @glycoshape.org, you know already aboutGlyTouCan IDs. Tune in ⬇️ to discover what GlyTouCan can do for your research 🥳

Carbohydrate-binding is a small profit affair, so proteins often adopt complex multidomain architectures enabling a mechanism known as 'binding cooperativity', where binding to one monomer contributes to the binding affinity of the whole systems BUT how does it actually work? 🔥 #glycotime 🧵1/4 ⬇️

Alexis Rose: I Don't Know You Tell Me (Schitt's Creek)

ALT: Alexis Rose: I Don't Know You Tell Me (Schitt's Creek)

static.klipy.com

A fab, sunshiny week in 🇩🇰! Great #glycotime 🧪 at the CBM16 in Helsingør, with posters Bea: regulation of N-glycosylation by OST (doi.org/10.1101/2025...) Silvia: Siglec-6 high-recision recognition of glycolipids (doi.org/10.1038/s420...) Ojas: de novo binders design (doi.org/10.64898/202...) 🧵⬇️

Daily job count is quite high these days after the release of our case study 1 week ago and of the ReGlyco de novo Binder Design Filter notebook ⬇️ doi.org/10.64898/202... and res. therein So exciting to see a growing number of researcher including glycosylation in their studies! 🎉 #glycotime 🧪

We are proud to be part of @elixiruknode.org as the @unisouthampton.bsky.social node, and to share our expertise in structural and computational glycoscience 🧪, together with our OA resources thought it. Check out the announcement below for more information ⬇️ 🥳

ELIXIR-UK@elixiruknode.org · 6mo ago

The University of Southampton has joined ELIXIR-UK 🎉 From open-access glycan databases to data stewardship, Southampton brings a wealth of expertise to the network. Read more: elixiruknode.org/news/2026/un... @elisafadda.bsky.social @glycoshape.org #openscience #bioinformatics #datastewardship

Most biologics are glycosylated and some of them heavily. In this “fresh off the press" #glycotime preprint we look into how a filter that accounts for glycosylation explicitly in 3D can help reduce lab costs and increase the efficiency of de novo binder design pipelines. Short 🧵 ⬇️ 1/7

bioRxivpreprint@biorxivpreprint.bsky.social · 6mo ago

Integrating glycosylation in de novo protein design with ReGlyco Binder Design Filter https://www.biorxiv.org/content/10.64898/2026.04.16.718906v1

Super congratulations to the Siglec (and many other stories) extraordinaire Dr. D'Andrea!!! 👩‍🎓🎉🥂🍾 A huge thank you to the fantastic examiners Alba Silipo (external) and Trinidad Velasco-Torrijos (internal) for your expert and in-depth examination, which made Silvia's viva a great day to remember 😎

Super congratulation to Dr Akash Satheesan from the glycoShape team, who graduated officially today with a PhD @maynoothuniversity.ie !! 😎🎓👏🥳🥂 The 🔺-red gown definitely suits you well Akash! 😍

Lectins are known to be low affinity binders with relatively broad glycan target preference. In this work live in @commsbio.nature.com we show how Siglecs change this paradigm, acting as molecular precision tools required to fine tune immune response 🤯 #glycotime 🧵⬇️ doi.org/10.1038/s420...

Phenomenal #glycotime by Lorenzo Rossi, @j-a-n-alexander.bsky.social, @asramirez.bsky.social and Kaspar Locher ⬇️ shows a pipeline for building THE glycoform you want with unprecedented yields! Now you can design your glycoform with GlycoShape and make it with Glyco-BUILD 👏 doi.org/10.1038/s414...

GLYCO-BUILD: an enzymatic pipeline for the synthesis of peptides carrying eukaryotic N-glycans - Nature Communications

Rossi and colleagues assemble an in vitro enzymatic pipeline using enzymes from distinct domains of life to recapitulate eukaryotic N-glycosylation. This work advances the synthesis of bespoke glycope...

doi.org

Happy new year 2026 #glycotime! 🥳 We just introduced some useful options to Re-Glyco Ensemble. In the Advanced Settings you can now select a seed (positive integer) to reproduce structural ensemble of your favourite glycoforms such as the one below that you will see soon in an upcoming paper,

Happy birthday to us! 🥂🎂🥳 We are only 2 yo, but so much #glycotime happened since Dec 2023! ⬇️🧵 We truly appreciate your support, you fabulous glycoengineers around the world and we have so many exciting new tools for you coming up in 2026 to make your 3D glycoforms even better and faster! 🤩

I love this thread by @glycoshape.org, this is @bsky.app at its best!

GlycoShape@glycoshape.org · 10mo ago

Super interesting work from @carolynbertozzi.bskyverified.social group about the effects of glycosylation in protein structure and function at the proteome scale 🤯💥🤩 Results show that sialylation and fucosylation are crucial, which makes a lot of sense. We did some extra bits 🧐 with GlycoShape ⬇️🧵

Super interesting work from @carolynbertozzi.bskyverified.social group about the effects of glycosylation in protein structure and function at the proteome scale 🤯💥🤩 Results show that sialylation and fucosylation are crucial, which makes a lot of sense. We did some extra bits 🧐 with GlycoShape ⬇️🧵

Carolyn Bertozzi@carolynbertozzi.bskyverified.social · 10mo ago

Congratulations former postdoc @jfhevler.bsky.social (in transit to Roche) and the team on their work using thermal proteome profiling along with glycosylation perturbagens to discover glycan-dependent protein functions at the proteome scale 👏 www.biorxiv.org/content/10.6...