⬇️ #glycotime 🧪

Elisa Fadda@elisafadda.bsky.social · 3w ago

Isn't it amazing when a fresh new preprint is out on Friday? 😎🥳 Check out the fabulous work by @beatricetropea.bsky.social who gives us a clear mechanistic explanation behind the 'peculiar' structure of the LLO donor preferentially chosen by eukaryotic OST 🤓😍 doi.org/10.64898/202...

The fully glucosylated LLO structure is the preferred substrate of eukaryotic OST, especially engineered to enhance N-glycosylation efficiency; but how does that work? and why do we need all those glucoses? @beatricetropea.bsky.social answers all these Q in a new preprint ⬇️ doi.org/10.64898/202...

How the terminal glucoside of the N-glycan donor affects the catalytic efficiency of the eukaryotic oligosaccharyltransferase

The eukaryotic oligosaccharyltransferase (OST) is the enzyme responsible for initiating N-glycosylation of secreted proteins by transferring a pre-assembled lipid-linked oligosaccharide (LLO) donor to...

doi.org

As a fun educational/inspirational #glycotime we decided to curate a Blog on glycoshape.org, you can find it under Resources or here glycoshape.org/blog You can *sign up* to get an email when we post a new entry The latest is E04:Let's talk about glycan binding to viral (glyco)proteins 🧪

Un-crystallisable glyco-complexes anyone? New work led by John Klassen's lab introduces antibody masking-native mass spectrometry (AM-nMS) a spatially resolved assay that allowed us to map glycan binding sites across the SARS-CoV-2 RBD. The results match our MDs! 😜 All details glycoshape.org/blog

glycoshape.org has now a blog where we discuss (yep you got it) glycans, glycoproteins and fun stuff! Season 1 is out with: Ep01: Why should we care about glycan heterogeneity? Ep02: How can we include glycan heterogeneity in a 3D framework? Ep03: Molecular precision glycoscience: Enter Siglecs

Next time you hear 'glycans are just a fancy protein decoration' 😱 show them this fabulous work led by Katrine Schjoldager and team at the CGR 🇩🇰 with us 🇬🇧 showing how The ligand preference of LRP1 is regulated by O-glycans Out now www.science.org/doi/10.1126/... 🧵1/10 ⬇️

The ligand preference of LRP1 is regulated by O-glycans

GALNT11-mediated O-glycans modulate LRP1 uptake of tau and Aβ, unveiling glycan-driven mechanisms in neurodegenerative pathways.

science.org

Glycoheterogeneity is an aspects of glycobiology that most would class into a category between difficult and intolerable, but @benschulz.bsky.social and us love it! So we wrote a whole review in Current Opinion in Structural Biology on it 🧵1/8 ⬇️ doi.org/10.1016/j.sb...

🚨 #glycotime seminar alert! June 22nd, 2026 4pm JST (8am BST) @kiyokof.bsky.social will present the GlyTouCan v.4 in the next GlySpace Alliance Seminar event 🔥 If you use @glycoshape.org, you know already aboutGlyTouCan IDs. Tune in ⬇️ to discover what GlyTouCan can do for your research 🥳

Carbohydrate-binding is a small profit affair, so proteins often adopt complex multidomain architectures enabling a mechanism known as 'binding cooperativity', where binding to one monomer contributes to the binding affinity of the whole systems BUT how does it actually work? 🔥 #glycotime 🧵1/4 ⬇️

Alexis Rose: I Don't Know You Tell Me (Schitt's Creek)

ALT: Alexis Rose: I Don't Know You Tell Me (Schitt's Creek)

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A fab, sunshiny week in 🇩🇰! Great #glycotime 🧪 at the CBM16 in Helsingør, with posters Bea: regulation of N-glycosylation by OST (doi.org/10.1101/2025...) Silvia: Siglec-6 high-recision recognition of glycolipids (doi.org/10.1038/s420...) Ojas: de novo binders design (doi.org/10.64898/202...) 🧵⬇️

Daily job count is quite high these days after the release of our case study 1 week ago and of the ReGlyco de novo Binder Design Filter notebook ⬇️ doi.org/10.64898/202... and res. therein So exciting to see a growing number of researcher including glycosylation in their studies! 🎉 #glycotime 🧪

We are proud to be part of @elixiruknode.org as the @unisouthampton.bsky.social node, and to share our expertise in structural and computational glycoscience 🧪, together with our OA resources thought it. Check out the announcement below for more information ⬇️ 🥳

ELIXIR-UK@elixiruknode.org · 4mo ago

The University of Southampton has joined ELIXIR-UK 🎉 From open-access glycan databases to data stewardship, Southampton brings a wealth of expertise to the network. Read more: elixiruknode.org/news/2026/un... @elisafadda.bsky.social @glycoshape.org #openscience #bioinformatics #datastewardship

Most biologics are glycosylated and some of them heavily. In this “fresh off the press" #glycotime preprint we look into how a filter that accounts for glycosylation explicitly in 3D can help reduce lab costs and increase the efficiency of de novo binder design pipelines. Short 🧵 ⬇️ 1/7

bioRxivpreprint@biorxivpreprint.bsky.social · 4mo ago

Integrating glycosylation in de novo protein design with ReGlyco Binder Design Filter https://www.biorxiv.org/content/10.64898/2026.04.16.718906v1

Super congratulations to the Siglec (and many other stories) extraordinaire Dr. D'Andrea!!! 👩‍🎓🎉🥂🍾 A huge thank you to the fantastic examiners Alba Silipo (external) and Trinidad Velasco-Torrijos (internal) for your expert and in-depth examination, which made Silvia's viva a great day to remember 😎

Super congratulation to Dr Akash Satheesan from the glycoShape team, who graduated officially today with a PhD @maynoothuniversity.ie !! 😎🎓👏🥳🥂 The 🔺-red gown definitely suits you well Akash! 😍

Lectins are known to be low affinity binders with relatively broad glycan target preference. In this work live in @commsbio.nature.com we show how Siglecs change this paradigm, acting as molecular precision tools required to fine tune immune response 🤯 #glycotime 🧵⬇️ doi.org/10.1038/s420...

Phenomenal #glycotime by Lorenzo Rossi, @j-a-n-alexander.bsky.social, @asramirez.bsky.social and Kaspar Locher ⬇️ shows a pipeline for building THE glycoform you want with unprecedented yields! Now you can design your glycoform with GlycoShape and make it with Glyco-BUILD 👏 doi.org/10.1038/s414...

GLYCO-BUILD: an enzymatic pipeline for the synthesis of peptides carrying eukaryotic N-glycans - Nature Communications

Rossi and colleagues assemble an in vitro enzymatic pipeline using enzymes from distinct domains of life to recapitulate eukaryotic N-glycosylation. This work advances the synthesis of bespoke glycope...

doi.org

Happy new year 2026 #glycotime! 🥳 We just introduced some useful options to Re-Glyco Ensemble. In the Advanced Settings you can now select a seed (positive integer) to reproduce structural ensemble of your favourite glycoforms such as the one below that you will see soon in an upcoming paper,

Happy birthday to us! 🥂🎂🥳 We are only 2 yo, but so much #glycotime happened since Dec 2023! ⬇️🧵 We truly appreciate your support, you fabulous glycoengineers around the world and we have so many exciting new tools for you coming up in 2026 to make your 3D glycoforms even better and faster! 🤩

I love this thread by @glycoshape.org, this is @bsky.app at its best!

GlycoShape@glycoshape.org · 8mo ago

Super interesting work from @carolynbertozzi.bskyverified.social group about the effects of glycosylation in protein structure and function at the proteome scale 🤯💥🤩 Results show that sialylation and fucosylation are crucial, which makes a lot of sense. We did some extra bits 🧐 with GlycoShape ⬇️🧵

Super interesting work from @carolynbertozzi.bskyverified.social group about the effects of glycosylation in protein structure and function at the proteome scale 🤯💥🤩 Results show that sialylation and fucosylation are crucial, which makes a lot of sense. We did some extra bits 🧐 with GlycoShape ⬇️🧵

Carolyn Bertozzi@carolynbertozzi.bskyverified.social · 8mo ago

Congratulations former postdoc @jfhevler.bsky.social (in transit to Roche) and the team on their work using thermal proteome profiling along with glycosylation perturbagens to discover glycan-dependent protein functions at the proteome scale 👏 www.biorxiv.org/content/10.6...

We just did some maintenance and updates to glycoshape.org, ✅ Fancy background with floating SNFG symbols 🤩 ✅ 'Latest Updates' feed from this account on bsky 🤩 ✅ Download PDB with SASA values in Re-Glyco Ensemble 🤩 Check it out!

For some unique #glycotime: C-man is a rare (but evolutionarily very old[1]) post translational modification of proteins, where the C2 of Trp in W-x-x-W/C sequons are linked to a single man through a C-C bond 🤯 This and its position within the structure stabilises the (unusual for man) 1C4 chair[2]

Tuesday #glycotime 🥄🍬 Glycans are a 🔝 example of "disordered" molecules. Inherent flexibility prevents sugars from adopting fixed, unique 3D positions relative to the crystal lattice, so they often are invisible to X-ray diffraction, unless they are bound and thus restrained in place 1/3 🧵

Today we are attending the @biochemsoc.bsky.social International Glycoimmunology Online Symposium, enjoying brilliant talks and discussions around the key role of glycans in regulating our immune system in health and disease Phenomenal #glycotime 👏👏🤩

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It's Fucose Friday 🔺, the best #glycotime of the week! Fucose is commonly found as a glycan modification, but it can be linked directly to proteins as O-fucose 😎 Here ⬇️ O-fucosylation of TSR1 by POFUT2 in the ER stabilises the TSRs characteristic fold 🤩 pmc.ncbi.nlm.nih.gov/articles/PMC...

To wish our friend and main O-glycan 3D structure contributor extraordinaire @silviadandrea.bsky.social all the best with the submission of her PhD thesis tomorrow, today's 🥄🍬 is dedicated to sialic acid 💜 with a mini 🧵 about all the wonderful functions Silvia worked on

Today we used Re-Glyco to rebuild human EPO from AF-P01588 with glycosylation selected from Lippold et al [10.1021/acs.analchem.0c01794] From the 3D structure we identified ideal locations for extra N-glycans, some of which are actually in NESP [10.1054/bjoc.2001.1746] Super fun 3D edu #glycotime!

To welcome our new friends, first spoonful of sugar 🥄🍬 Glycan structures can be very complex and deceivingly similar, yet they have unique GlyTouCan IDs[1] that you can find by drawing the glycan you need in gb.glytoucan.org or in the glycoshape.org sugar drawer 1/3 🧵

Hello bsky 🦋 GlycoShape is now here to give you updates on our glycan 3D database, toolbox, glyco fun-facts and news! We just updated server, so clear your browser's cache, and build some glycoproteins with us 🍬 We'll have great new tools coming up soon, so follow us for some exciting #glycotime

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