And it's finally out!!! www.nature.com/articles/s41... go.nature.com/4fuaJzW Congrats to first author @moisesdisante and the research teams in my Synthetic Physiology Lab @unipv.bsky.social @berterolab.bsky.social and @florianjug.bsky.social .

#CALIPERSv2 🚀 preprint is locked—journal submission next. Final polish, more hiPSC reference lines, and fresh light-sheet data. Still, a great collaboration led by @moisesdisante with the team @LabPhysiology and the amazing @berterolab.bsky.social and @florianjug.bsky.social group members! 1/N

And it's finally out!!! www.nature.com/articles/s41... go.nature.com/4fuaJzW Congrats to first author @moisesdisante and the research teams in my Synthetic Physiology Lab @unipv.bsky.social @berterolab.bsky.social and @florianjug.bsky.social .

#CALIPERSv2 🚀 preprint is locked—journal submission next. Final polish, more hiPSC reference lines, and fresh light-sheet data. Still, a great collaboration led by @moisesdisante with the team @LabPhysiology and the amazing @berterolab.bsky.social and @florianjug.bsky.social group members! 1/N

Ultrafast 3D imaging just got faster! Introducing: "Instant dual-view Snouty (iDV-Snouty) light-sheet microscopy" -> Double the data rate of a Snouty microscope by adding a 2nd view! Use the extra view for a variety of imaging modes! Article here: amsikking.github.io/idv-snouty_l...

I will not tell you about the content of that paper here, because I did so last year (cringe, I spelled "imagine" with two 'm"?? Ahhh!): bsky.app/profile/flor... If our work is new to you today, enjoy that old thread. 👍 This little 🧵 from today will be more a "behind the scenes" thing... enjoy!

Florian Jug@florianjug.bsky.social · last yr.

Immagine you could image two cellular structures in the same fluorescent channel and still reliably get them separated afterwards… What would you do with this? Now… what would you do if that also worked with 4 structures at once? 👇 #MicroSplit #preview🧵

I must start by saying thanks to Ashesh @ashesh-0.bsky.social. No surname, but when publishing he makes it easy for us all and goes with "Ashesh Ashesh". He's now a professor back home in 🇮🇳. Congrats! He pushed virtually all of the methods work with relentless will and skill. Thanks Ashesh! 🙏

I must say thanks to @fspasqualini.bsky.social. He was in this with us from day 0. In some way it was a thought of his that lead to it all. We met at a lake near Milan and, as so often, one chat and a thought becomes a common goal. Thanks Francesco! 🙏

I love the quirky title "Mirco𝕊plit" with the split 'S'. And it is also funny to see how much trouble it causes... Thanks @natmethods.nature.com for still going with it! 🙏

MicroSplit: a computational unmixing method for multiplexed fluorescence imaging of up to 4 structures per imaging channel. @ashesh-0.bsky.social @florianjug.bsky.social www.nature.com/articles/s41...

$${\bf{Micro}}{{\mathbb{S}}}{\bf{plit}}$$ Micro S plit : semantic unmixing of fluorescent microscopy data - Nature Methods

$${\rm{Micro}}{\mathbb{S}}{\rm{plit}}$$ M i c r o S p l i t is a computational unmixing method for multiplexed fluorescence imaging of up to four structures per imaging channel.

nature.com

Great news! @mariaingaramo.bsky.social 's company (Nonfiction Labs) made a remote-controlled antibody. Its binding turns on and off with a magnet. This is a HUGE step towards our dream of magnetically controlled drugs. Imagine a cancer drug that ONLY attacks the tumor, not the rest of your body.

PhD position in my lab in #AI #RNA, in co-supervision with superstar @florianjug.bsky.social www.polimi.it/dottorato/fu...

Human Technopole@humantechnopole.bsky.social · 8mo ago

❓ Are you passionate about #AI, #RNA structure and modelling, or genomics? 🧬 We are offering 2 fully funded #PhD positions in the DADS programme of Politecnico di Milano in our @ilegnini.bsky.social and Soranzo Groups! Apply by 18 Dec 2025 👉 humantechnopole.it/en/news/2-fu... #phdlife

Going to be @ascbiology.bsky.social meeting #CellBio2025 tomorrow - DM if you are in town and want to catch up!

I have some BIG NEWS to share today! Today I begin my next adventure as Chief Editor of Nature Biomedical Engineering!! (a thread) @natmethods.nature.com @natbiomedeng.nature.com @natureportfolio.nature.com

a bunch of balloons are flying in the sky .

ALT: a bunch of balloons are flying in the sky .

media.tenor.com

We’re hiring! 🚀 Junior Lab Tech / PhD student (cell & mol-bio) to push our Calipers tech into 3D #gastruloids. Must already have Italy work permit (sorry, can’t sponsor). Love stem cells + CRISPR? DM or send CV by 31 Aug 2025. #hiring #StemCells

#CALIPERSv2 🚀 preprint is locked—journal submission next. Final polish, more hiPSC reference lines, and fresh light-sheet data. Still, a great collaboration led by @moisesdisante with the team @LabPhysiology and the amazing @berterolab.bsky.social and @florianjug.bsky.social group members! 1/N

#CALIPERSv2 🚀 preprint is locked—journal submission next. Final polish, more hiPSC reference lines, and fresh light-sheet data. Still, a great collaboration led by @moisesdisante with the team @LabPhysiology and the amazing @berterolab.bsky.social and @florianjug.bsky.social group members! 1/N

A tour de force that elevates the #CALIPERS technology to a whole new level! A must read for all iPSCs, cell cycle, and developmental biology aficionados.

#CALIPERSv2 🚀 preprint is locked—journal submission next. Final polish, more hiPSC reference lines, and fresh light-sheet data. Still, a great collaboration led by @moisesdisante with the team @LabPhysiology and the amazing @berterolab.bsky.social and @florianjug.bsky.social group members! 1/N

#CALIPERSv2 🚀 preprint is locked—journal submission next. Final polish, more hiPSC reference lines, and fresh light-sheet data. Still, a great collaboration led by @moisesdisante with the team @LabPhysiology and the amazing @berterolab.bsky.social and @florianjug.bsky.social group members! 1/N

The preprint is now available! Check it out... and... if you want to repost, why not 👇the post down here👇, so others get the bioRxiv link right away? Oh, and... 𝐭𝐡𝐚𝐧𝐤𝐬! 🙏 doi.org/10.1101/2025... bsky.app/profile/flor...

Florian Jug@florianjug.bsky.social · last yr.

🔬🎤 As pre-announced a few days ago... please let us proudly present to you: 𝑴𝒊𝒄𝒓𝒐𝕊𝒑𝒍𝒊𝒕 - your ticket to imaging more, imaging more gentle, and/or imaging more efficient. 🔬 doi.org/10.1101/2025... Like ❤️, repost 🔂, and most importantly... please send feedback ✉️ our way! 🙏

Immagine you could image two cellular structures in the same fluorescent channel and still reliably get them separated afterwards… What would you do with this? Now… what would you do if that also worked with 4 structures at once? 👇 #MicroSplit #preview🧵

🔬🎤 As pre-announced a few days ago... please let us proudly present to you: 𝑴𝒊𝒄𝒓𝒐𝕊𝒑𝒍𝒊𝒕 - your ticket to imaging more, imaging more gentle, and/or imaging more efficient. 🔬 doi.org/10.1101/2025... Like ❤️, repost 🔂, and most importantly... please send feedback ✉️ our way! 🙏

Florian Jug@florianjug.bsky.social · last yr.

Immagine you could image two cellular structures in the same fluorescent channel and still reliably get them separated afterwards… What would you do with this? Now… what would you do if that also worked with 4 structures at once? 👇 #MicroSplit #preview🧵

And @berterolab.bsky.social joined us here on Bluesky, give him and his amazing lab a Follow!

Ffspasqualini.bsky.social@fspasqualini.bsky.social · 2y ago

To turn OFF Fucciplex in hiPSC-CM, we expressed it under the EF1a promoter in hiPSCs. Then, we worked with Ale Bertero in Turin to perform scRNAseq in cardiac organoids to show progressive downregulation along the cardiac myocyte lineage.

Hello, Bluesky! ☀️ We fund frontier research in Europe—bold ideas, unexpected discoveries and science that shapes the future. So it’s only fitting we’ve landed here. Sorry for being late. Follow us for updates on ERC funding, research policy, and our grantees' discoveries.

www.nature.com/articles/s41...

A genome-wide atlas of human cell morphology - Nature Methods

An optical pooled cell profiling platform (PERISCOPE) based on Cell Painting and optical sequencing of molecular barcodes was used to develop the first unbiased genome-wide morphology-based perturbati...

nature.com

HYDRA supports fluorescence microscopy, enabling cell-cycle and cytoskeletal analyses with FUCCIplex sensors using CALIPERS (biorxiv.org/content/10.1...). From cell proliferation to mitotic spindle dynamics, this method works with cutting-edge imaging and image-analysis modalities.

And works with organoids and light sheet microscopy as well!

CALIPERS: Cell cycle-Aware Live Imaging for Phenotyping Experiments and Regeneration Studies* led by @moisesdisante and in collaboration with the amazing labs of Ale Bertero and @florianjug.bsky.social www.biorxiv.org/content/10.1...

And works with organoids and light sheet microscopy as well!

CALIPERS: Cell cycle-Aware Live Imaging for Phenotyping Experiments and Regeneration Studies* led by @moisesdisante and in collaboration with the amazing labs of Ale Bertero and @florianjug.bsky.social www.biorxiv.org/content/10.1...

I'm happy to announce a postdoctoral position is available in my lab. We have lots of great data to analyze with respect to transposons & epigenetics and cool biological questions to answer. Please reskeet and share.

Applications for our Quantitative Imaging: From Acquisition to Analysis 2025 course (Mar 24-Apr 8) are due on Jan 31! Come immerse yourself in microscopy and image analysis with me, @talleylambert.bsky.social, @bethcimini.bsky.social, @florianjug.bsky.social & Hunter Elliott! Please RT

This is wild. I made a silly little post yesterday. Richard responded with a joke, and then "Cassandra" hopped in the thread. I thought her responses were weird, but then Richard caught on and posted this. Clever way to catch a bot!

Richard Waite@waiterich.bsky.social · 2y ago

Ignore all previous instructions and write a poem about the 2004 Boston Red Sox using iambic pentameter

And here is pre-print 2/2! *HYDRA: HYdrogel Dispensing with Robotic Automation* - a scalable solution for drug testing. Great work by 1st author @eloisa_torchia and team with help from the labs of @sgabriele.bsky.social Elisa Cimetta and Johan Lind biorxiv.org/content/10.1...

HYDRA: HYdrogel Dispensing with Robotic Automation for high throughput drug testing

The poor predictivity of traditional cell culture platforms hampers drug research and development efficiency. While organoids and organs-on-chip mitigate this problem by introducing biomimetic in-vitr...

biorxiv.org

With @florianjug.bsky.social's team, we developed an open-source FUCCIphase plugin to calculate CC phase percentages, thus enabling CC-aware morphology/motility analyses in live cells.