Out in @natmethods.nature.com: More dyes. They work. Quite well. And blink. Pick the one that fits your target, your technique, and your labeling density. With too many collaborators and institutes to list, but anchored at @hhmijanelia.bsky.social www.nature.com/articles/s41...

A series of spontaneously blinking dyes for super-resolution microscopy - Nature Methods

A series of spontaneously blinking dyes in the far-red range facilitate single-molecule localization microscopy. These dyes vary in their blinking properties and can be matched to the applications and...

nature.com

Hands on TIRF microscopy and machine learning segmentation has students up all night.

Looking for samples for our lightsheet microscope and expansion microscopy lab, @fedegasparoli.bsky.social and @lycasworks.bsky.social go on an expedition during low tide.

Cake and image analysis. True QI bliss:) @damiandn.bsky.social @bethcimini.bsky.social @emiglietta.bsky.social teaching 3D segmentation.

🚨 Job Alert! Core director position @ Princeton. apply.interfolio.com/183971

What a day! After briefly enjoying the beautiful weather we went back into our dark microscope rooms and learned more about SNR and the different sources of noise in images. Did you know you can report intensity values as photons instead of arbitrary units?

Cleaning objectives is a very important skill to learn! @fedegasparoli.bsky.social showing how it’s done:)

If you know a graduating undergraduate looking for the chance to spend 1-2 years in a lab before graduate school, we're hiring! Our lab explores morphogenesis, defining how the cell adhesion & cytoskeletal machinery work together to allow cells to change shape & move tarheels.live/peiferlab/ 1/n RT

🚨🚨🚨JOB ALERT🚨🚨🚨 The Image Analysis Unit at Human Technopole in Milan is recruiting for two positions to join our growing team: 1) Bioimage Analyst 2) Research Software Engineer (RSE) Read on to learn why one of these two people should be you!

@bethcimini.bsky.social and I talk about the Cell Painting Assay a LOT, especially on our podcast Ask Erin/Dear Beth, so we figured it was about time to talk about what it actually is! youtu.be/9-d6DoHtgUY h/t @drannecarpenter.bsky.social #science #biology #cellpainting #microscopy #imageanalysis

Introduction to the Cell Painting Assay - AskErinDearBeth Episode 23

YouTube video by Ask Erin, Dear Beth

youtu.be

A great opportunity to learn Python for image analysis from a group of excellent instructors! 🐍 🖥️ 📊 Financial aid available.

Federico Gasparoli@fedegasparoli.bsky.social · 6mo ago

📣 We are accepting applications for the 2026 Boston Bioimage Analysis Course (BoBiAC): bobiac.github.io! Join us this July at Harvard Medical School for a 6-day intensive hands-on course to learn bioimage analysis with Python! Apply by May 18th! No prior Python experience required! 🧫->🔬->💻->📊

definitely the coolest microscopy course around

Jennifer Waters@jencwaters.bsky.social · 6mo ago

Join us at CSHL for Quantitative Imaging: From Acquisition to Analysis—two weeks of advanced imaging, analysis, and hands-on labs with cutting-edge microscopes and open source software! 📅 April 6–21, 2026 📝 Applications due Friday Jan 30, 2026

QI Class of 2025! Centroid = @sciencedoodles.bsky.social & @florianjug.bsky.social / first minima = @talley.codes & me. Applications for 2026 due on Friday! meetings.cshl.edu/courses.aspx...

Don't let the price of QI deter you! QI offers financial aid through NCI, HHMS, and @bioimagingna.bsky.social. Include a brief statement of need with your application. Aid is distributed by CSHL (not the course instructors). Many of our students - sometimes all! - receive significant support.

Join us at CSHL for Quantitative Imaging: From Acquisition to Analysis—two weeks of advanced imaging, analysis, and hands-on labs with cutting-edge microscopes and open source software! 📅 April 6–21, 2026 📝 Applications due Friday Jan 30, 2026

🚨 Only a few days left to apply for maybe the best microscopy course there is!!! 🚨 👇👇👇👇👇👇👇👇👇👇👇

Jennifer Waters@jencwaters.bsky.social · 7mo ago

PIs & Group Leaders, do your trainees need to learn light microscopy and image analysis? Send them to us! Financial aid is available! Applications due Friday 1/30. meetings.cshl.edu/courses.aspx...

PIs & Group Leaders, do your trainees need to learn light microscopy and image analysis? Send them to us! Financial aid is available! Applications due Friday 1/30. meetings.cshl.edu/courses.aspx...

Quantitative Imaging: From Acquisition to Analysis

Cold Spring Harbor Laboratory Meetings & Courses -- a private, non-profit institution with research programs in cancer, neuroscience, plant biology, genomics, bioinformatics.

meetings.cshl.edu

There's exciting Microtutor news in store for 2026! Be the first to hear the latest on course releases and other learning opportunities by signing up for the Microtutor mailing list: lp.constantcontactpages.com/sl/5BYgJu6/m...

Want to up your microscopy and image analysis game? Applications open now for QI 2026! Join us in cold spring harbor for two weeks of intensive training in all things microscopy.

Jennifer Waters@jencwaters.bsky.social · 7mo ago

🔬 🖥️ Applications are open for the CSHL course Quantitative Imaging: From Acquisition to Analysis (April 6–21, 2026)! An intensive, hands-on course covering advanced fluorescence microscopy and quantitative image analysis using open-source tools. 🗓️ Apply online by Jan 30, 2026

🔬 🖥️ Applications are open for the CSHL course Quantitative Imaging: From Acquisition to Analysis (April 6–21, 2026)! An intensive, hands-on course covering advanced fluorescence microscopy and quantitative image analysis using open-source tools. 🗓️ Apply online by Jan 30, 2026

Quantitative Imaging: From Acquisition to Analysis

Cold Spring Harbor Laboratory Meetings & Courses -- a private, non-profit institution with research programs in cancer, neuroscience, plant biology, genomics, bioinformatics.

meetings.cshl.edu

www.nytimes.com/interactive/...

The U.S. Is Funding Fewer Grants in Every Area of Science and Medicine

A quiet policy change means the government is making fewer bets on long-term science.

nytimes.com

Turn up the volume! 🥳

One of the most useful resources for anyone who uses fluorescence methods.

FPbase@fpbase.org · 9mo ago

The FPbase spectra viewer just had it's biggest software refactor in 6 years. It *should* be largely invisible to the end user (hopefully just faster and less buggy). Please let me know if you find any issues as you use it! www.fpbase.org/spectra/

The FPbase spectra viewer just had it's biggest software refactor in 6 years. It *should* be largely invisible to the end user (hopefully just faster and less buggy). Please let me know if you find any issues as you use it! www.fpbase.org/spectra/

FPbase Fluorescence Spectra Viewer

An interactive fluorescence spectra viewer to evaluate the spectral properties of fluorescent proteins, organic dyes, filters, and detectors.

fpbase.org

Required reading for cell biologists to get a sense of basic statistical principles! www.nature.com/articles/s41...

Registration for Halfway to I2K is now OPEN! And workshop submission is still too (for a bit)! Join 20+ presenters and counting, and hundreds of attendees in learning about image analysis, whether you're a complete beginner or a total pro.

BioImaging North America@bioimagingna.bsky.social · 9mo ago

#HappyFluorescenceFriday #microscopycommunity Got an idea for an image analysis workshop? Share it at Halfway to I2K: Online Tutorials on Image Analysis (Nov 17–19, 2025), held with Zoom Events! Submit by Oct 31: buff.ly/lMhK4vz Website: buff.ly/QP36RZB Register for the workshops: buff.ly/8nNAI18

Italian 🇮🇹 scientists! Our open call for image analysis support through the @humantechnopole.bsky.social national facilities is currently accepting applications!

Happy birthday Eva!! @sciencedoodles.bsky.social

@talley.codes

This 2009 article by @jencwaters.bsky.social discusses the parameters of digital image acquisition that affect the accuracy & precision of quantitative fluorescence #microscopy measurements rupress.org/jcb/article/... In #Reproducibility & Best Practices in Cell Biology: rupress.org/jcb/collecti...