Sheela Abraham
@haemev.bsky.social
Haematopoietic stem cell enthusiast, leukaemia hater, extracellular vesicles are key, Canadian mom to sulky British teen, Researcher at Queen’s. Did my time at UBC, Cambridge, Glasgow. Art, wine and culture fuel me
Please check out our newest publication identifying a new function of extracellular vesicles! dx.doi.org/10.1002/jev2...
Extracellular Vesicles Define Discrete Nano‐Based Niches Within the Human Haematopoietic System
Stem cell niches are complex multi-signalling networks comprised of molecular cues and physical interactions, orchestrated by niche-resident cells and the extracellular factors they produce. The bone....
dx.doi.org
Do you love EVs? How about blood, bones and gore? Please check out our latest protocol paper outlining how to enrich for human bone EVs! www.biorxiv.org/content/10.1...
Enriching for Extracellular Vesicles from Human Bone
Extracellular vesicles (EVs) are nano-sized membrane-bound structures thought to be secreted by all cells and increasingly recognized as key mediators of intercellular communication. Established EV isolation protocols for bodily fluids-primarily focus on blood with limited insights into methods optimized for EVs from other hematopoietic regions. In this study, we present a novel protocol for the isolation and enrichment of EVs from human trabecular bone and bone marrow. This method employs a two-step purification strategy, combining iodixanol density cushion (IDC) ultracentrifugation with size exclusion chromatography (SEC), and enables EV recovery from fresh tissue hours after collection. Importantly, this approach facilitates the enrichment of bone-derived EVs without the need for enzymatic digestion or long-term culture, preserving native EV populations. This protocol offers a valuable tool for researchers investigating EVs derived from the diverse cellular constituents of the bone microenvironment. ### Competing Interest Statement The authors have declared no competing interest. Canadian Institutes of Health Research, https://ror.org/01gavpb45, 394568 Terry Fox Research Institute, https://ror.org/00mtf6c40, 1133
biorxiv.org
Excited to see the first commentary in Experimental Hematology (@isehsociety.bsky.social)! Kudos to Aishwarya, Linde @lindemileslab.bsky.social, and Grant @challenlab.bsky.social for highlighting recent papers in Exp Hem on cell-extrinsic factors in Tet2-mutant clonal hematopoiesis!! bit.ly/4k3pEAI
Cell-extrinsic factors contribute toward myeloid-biased expansion in Tet2-mutant clonal hematopoiesis
Hematopoietic stem cells (HSCs) acquire somatic mutations throughout life, with the frequency of mutational incidence increasing with age [1]. Occasionally, certain mutations confer a fitness advantag...
bit.ly
Called many places home but at heart, always Albertan. I was raised to care for everyone-vulnerable, sick, young and old. I voted for Carney. We are Canadian. #ElbowsUp
Sonya 🇨🇦@icedsnowberry.bsky.social · last yr.I love Alberta. Born & raised in Calgary, now raising 2 kids here. I am Canadian first 🇨🇦 I voted for Carney. #ElbowsUp
I love Alberta. Born & raised in Calgary, now raising 2 kids here. I am Canadian first 🇨🇦 I voted for Carney. #ElbowsUp
Me in the national security group chat:
Cocoa Deluxe@seauxcocoa.bsky.social · last yr.Me in the national security group chat:
Delighted to share my PhD thesis work is now published in @nature.com! This study tracks the journey of blood stem cells in aging mice, examining how the clonal dynmics of blood production change over time. A short 🧵1/n #aging #stemcells #clonalhematopoiesis www.nature.com/articles/s41...
Clonal dynamics and somatic evolution of haematopoiesis in mouse - Nature
Isolating and studying haematopoietic stem cells in young and aged mice demonstrates evolutionary processes related to blood production and provides a framework for interpreting future work using labo...
nature.com
Freedom and democracy are always worth fighting for. Slava Ukraini.
🔥 New Fraticelli lab publication🔥 “Pre-existing stem cell heterogeneity dictates clonal responses to the acquisition of leukemia driver mutations” Now at Cell Stem Cell, with two new figures, in vivo, and sequential mutagenesis data. Performed with the support of Cris Cancer and @erc.europa.eu
I think people both within science and outside science don’t fully appreciate the miracle that were the Covid-19 vaccines. How much courage it took to get the sequence out, how much work had already gone into figuring out the best viral antigen and how best to present it. 1/
Anyone know of a site for posting excuses from students to re-do exams/assignments? Its exhausting being inundated with requests for extra marks to the detriment of other students. I won't even start with the 30% accommodations that already exist. I fear for the next generation workforce..
Two big reveals in the world of #extracellularvesicles this week: Blebbisomes www.nature.com/articles/s41... and the ‘torn bag mechanism’ of small EV release from amphiectosomes elifesciences.org/articles/958...
Blebbisomes are large, organelle-rich extracellular vesicles with cell-like properties - Nature Cell Biology
Jeppesen, Sanchez et al. identify blebbisomes as large membrane-enclosed vesicles containing organelles and secreted by cells. Blebbisomes can both secrete and internalize exosomes and microvesicles.
nature.com
Super intriguing findings
Simon Haas@simonhaas.bsky.social · last yr.Ever wondered what drives hematopoietic stem cell (HSC) heterogeneity? In this preprint, our collaborative team uncovered that differences in HSC reconstitution kinetics play a key role in shaping stem cell heterogeneity and lineage biases. doi.org/10.1101/2025... (1/2)
A beautiful reminder when in the midst of human created chaos
So happy to see this video after such a disheartening week of politics. Proud teary Canadian
Brad Wouters 🇨🇦@handle.invalid · last yr.
Incredibly moving Justin Trudeau remarks: "We have fought and died alongside you....During your darkest hours...we were always there. Standing with you, grieving with you, the American people....Canadians are a little perplexed as to why our closest friends and neighbors are choosing to target us."
Finally online! In our most recent review, super-resolution microscopy meets mitochondria...and vice-versa 😀. Great effort led by Nicolas, postdoc in the team! Hope you'll enjoy it! onlinelibrary.wiley.com/doi/10.1111/...
Revealing mitochondrial architecture and functions with single molecule localization microscopy
Conventional microscopy techniques are limited by a resolution limit of 250 nm, making it challenging to visualize subcompartment structures. Single molecule localization microscopy (SMLM) has revolu...
onlinelibrary.wiley.com
Reminder that @europepmc.org is a mirror of Pubmed with better search and that it indexes *all* bioRxiv preprints (as well as ResearchSquare), not just the ones funded by NIH like chauvinist Pubmed
Prasad Jallepalli, MD, PhD@prasad.bsky.social · 2y agoI’m very scared to see what happens to PubMed
Is pubmed shut down? Has Trump finally cut science at the knees?
We all need to stand on guard for thee…
Catherine McKenna@cathmckenna.bsky.social · 2y agoI simply cannot believe that all Canadian politicians are not 💯 on #TeamCanada. I’m talking about you, Poilievre and your team, and you, Premier Smith. This isn’t a joke. We face a massive threat to 🇨🇦 economy, to 🇨🇦 workers, to 🇨🇦 sovereignty. It’s shameful.
Not sure how to cope without my 3-4pm Christmas cookie break #sundayblues
Some exciting to funding to advance patient-centric cancer research by some of my colleagues at Sinclair Cancer Research Institute bit.ly/3VIBO7W
Queen’s team part of groundbreaking new, patient-directed research program
Doctors and researchers in Kingston as well as cancer clinics and labs across the country will spend the next year following “patients’ orders” rather than the other way around after receiving funds f...
bit.ly
Purely coincidental, but seems poetic that the day after it's announced that Terry Fox will be on the Canadian $5 bill, the Marathon of Hope Cancer Centres Network that he inspired released the results of the Patients Voice in Research competition. www.marathonofhopecancercentres.ca/impact/story...
How a new program designed by patients, survivors and caregivers is paving the way for patient-centric research
marathonofhopecancercentres.ca
Life’s problems don’t seem so bad if someone hands you a pink frothy cocktail with a wee flower
