Please make sure to attend! Zoom-ID: ID: 961 7810 6979 Passcode: DGZ_FW I will present how we captured vesicle fusion in neurons using optogenetics for timed cryo-ET.

Fran Bottanelli@franbottanelli.bsky.social · 6mo ago

Join us next Tuesday for the next @dgz.bsky.social workshop on #trafficking and EVs! Spread the word please!

Join us next Tuesday for the next @dgz.bsky.social workshop on #trafficking and EVs! Spread the word please!

Please share🙏: Our lab opens 2 PhD positions to investigate fungi's evolution: #Barcelona Wet lab in Biochemistry/Structural Biology of cryo-tolerant fungi www.upf.edu/documents/d/... Computational in Physical Chemistry of cellular processes under extrem environments www.upf.edu/documents/d/...

🔬🔦 Imaging spotlight on FocalPlane @janakroll.bsky.social and colleagues highlight their research visualising synaptic vesicle fusion using cryo-ET combined with plunge freezing and optogenetics. focalplane.biologists.com/2026/01/21/i...

Imaging spotlight: visualising synaptic vesicle fusion by cryo-ET - FocalPlane

Imaging spotlight: visualising synaptic vesicle fusion by cryo-ET - News

focalplane.biologists.com

❄️ With all the snow outside, I felt like sharing a cryo tomogram: A neuronal synapse, captured by cryo-ET at the moment a neurotransmitter-filled vesicle fuses at the active zone. Fusion was triggered optogenetically and arrested by plunge freezing. More details: www.nature.com/articles/s41...

Der Moment, in dem eine Nervenzelle ihre Neurotransmitter in den synaptischen Spalt ausschüttet, ist extrem kurz. Berliner Forschenden @bihatcharite.bsky.social @mdc-berlin.bsky.social konnten ihn mikroskopisch einfangen. doi.org/10.1038/s414... Bild: © Charité/Max Delbrück Center, Jana Kroll

A breathtaking glimpse into the #brain at work: NeuroCure researchers & colleagues @janakroll.bsky.social captured #synapses in action - revealing how #neurotransmitters are released in milliseconds 🧠 Published in NatureCommunications @Charite @mdc-berlin.bsky.social www.nature.com/articles/s41...

Dynamic nanoscale architecture of synaptic vesicle fusion in mouse hippocampal neurons - Nature Communications

Synaptic vesicle fusion is essential for neuronal communication, yet its nanoscale sequence has not been directly confirmed. Here, the authors use timed in situ cryo-electron tomography to visualize t...

nature.com

❄️🔬⏱️ I am excited to share that our study „Dynamic nanoscale architecture of synaptic vesicle fusion in mouse hippocampal neurons” has been published in Nature Communications www.nature.com/articles/s41... 👇 Short summary of our key findings (1/8)

💥I am excited to present our first research paper from the lab about caveolae lipid trafficking 🎉 Led by my fantastic PhD candidate @estherocket.bsky.social Here we looked into caveolae mediated dietary lipid uptake and how this process is mechanistically facilitated 👇 1/n

bioRxivpreprint@biorxivpreprint.bsky.social · 8mo ago

Lipid-induced Caveolin1-Lipid droplet trafficking is associated with lipid droplet growth https://www.biorxiv.org/content/10.64898/2025.12.10.693432v1

We got a large Helmholtz Bioengineering grant with Oliver Daumke, Artur Yakimovich, Alina Bazarova, and Dietrich Ruess to use protein design to target cancer. Five open postdoctoral positions in Generative AI & Protein Engineering. Share & apply! jobrxiv.org/job/max-delb...

Postdoctoral Positions in Protein Engineering

Post a job in 3min, or find thousands of job offers like this one at jobRxiv!

jobrxiv.org

Group Leader in Biomedical Engineering at the @mdc-berlin.bsky.social in Berlin. It's a broad call for whoever is doing exciting Bioengineering, please apply or share. We have a great institute with amazing colleagues and core facilities,highly recommend application.mdc-berlin.de/site/index.p...

Postdoc program at the MDC in Berlin, @mdc-berlin.bsky.social : take a look 👇👇 For this call, collaborative projects between two groups will be funded. Take a look at the call and the groups and drop me an email if interested. Share with colleagues too! www.mdc-berlin.de/postdocs#t-g...

🏆 Images from #mdcBerlin received top honors in the @helmholtzimaging.bsky.social “Best Scientific Image Contest”! Jury Award for @janakroll.bsky.social, Daumke Lab Public Choice Award for Dr. Rafael Deliz Aguirre, @coscialab.bsky.social Full details: www.mdc-berlin.de/news/news/tw...

We have a preprint for you EHD2 forms rings on caveolae necks, in contrast to most EHDs forming helices. We determined its structure on membranes and show that N-term acts as a spacer By Elena, Vasya @vasiliimikirtumov.bsky.social, Jeff &Oli Daumke www.biorxiv.org/content/10.1...

What an inspiring morning at the CatCat Symposium! Thank you so much for inviting me @gallegolab.bsky.social and @felixmendu.bsky.social, it's been a pleasure visiting you in Barcelona 😊

So many amazing images! 🤩 🤩🤩 Please make sure to vote, mine is also in the list 😎

Helmholtz Imaging@helmholtzimaging.bsky.social · last yr.

🎉 Public Voting Now OPEN – #HelmholtzImaging Best Scientific Image Contest 2025 Choose your favorite(s) & cast your vote by May 30 👉 bit.ly/BSIC2025-Public-Voting The award recipients will be announced at our conference on June 26. #HIBestImage25 #HIConference25 @www.helmholtz.de

A broad call for a PhD researcher/ postdoc in our group at the MDC in Berlin. An ideal PhD student - a computational scientist, a postdoc - a structural biologist, but feel free to re-convince me! We extended the deadline for after Easter, Apr 24th. Apply here www.mdc-berlin.de/career/jobs/...

We are looking for a PhD student/Postdoc to develop methods of cryo-ET and/or study the structure and function of membrane proteins. Great team at the MDC in Berlin. It's a broad call, please apply and share! jobrxiv.org/job/max-delb...

PhD /Postdoc in In Situ Structural Biology by Cryo-ET

Post a job in 3min, or find thousands of job offers like this one at jobRxiv!

jobrxiv.org

Together with @gallegolab.bsky.social we are organizing the next CATCAT morning on "Dynamics in biology, from molecules to tissues", with an amazing lineup of local and international speakers. If in Barcelona on May 26th, don't miss it, it's free! www.catcat-celltissuebiology.cat/event/9th-ca...

Cryo-confocal microscopy of a neuron expressing the glutamate sensor iGluSnFR 🥶 The neuron was optogenetically stimulated and subsequently plunge-frozen to preserve the high fluorescence intensity indicating neurotransmitter release. #FluorescenceFriday

Excited to share our new preprint "Dynamic nanoscale architecture of synaptic vesicle fusion in mouse hippocampal neurons" www.biorxiv.org/content/10.1... We combine optogenetic stimulation of neurons with in situ cryo-ET to characterize membrane dynamics during synaptic vesicle fusion ❄️🔬⏱️

It's been a pleasure presenting my project at this fantastic workshop! ☺️ #EMBOinsitu

EMBL Events@events.embl.org · last yr.

#EMBOinsitu Session 4: Single cell systems ➡️ 'Dynamic nanoscale architecture of synaptic vesicle fusion in mouse hippocampal neurons' 🎙️ Jana Kroll – Max Delbrück Center for Molecular Medicine, Germany @mdc-berlin.bsky.social

For me, the best part of research is discovering unexpected things. That’s exactly what happened while working on this project. I’m glad to see our story come to completion and excited about how many new questions it opens up! www.pnas.org/doi/10.1073/...

Oh my. This is just gorgeous from Misha Kudryashev's lab (I don't think he's here yet?). Love to see it. V ATPase resolved directly in synaptic vesicles 🤤 Lots of other neat observations in there also. Congrats to all involved! www.pnas.org/doi/10.1073/...

Researchers in Misha Kudryashev's lab at the #mdcBerlin have used cryo-electron tomography to uncover new details of the molecular structure of synaptic vesicles, which help transport neurotransmitters in the brain. The study was published in @pnas.org. More 👇 www.mdc-berlin.de/news/press/i...

Imaging synaptic vesicles in 3D

Researchers at the Max Delbrück Center have used cryo-electron tomography to uncover new details of the molecular structure of synaptic vesicles, which help transport neurotransmitters in the brain. T...

mdc-berlin.de