1/ Very excited to share the first publication from my lab! In this work, we developed an efficient strategy to precisely mutate mammalian histone genes in their native genomic context using CRISPR prime editing: www.nature.com/articles/s41...

Identifying critical lysines in mammalian histone H3 with high-throughput CRISPR prime editing - Nature Genetics

This study uses a precise and efficient clustered regularly interspaced short palindromic repeats (CRISPR) prime editing system to substitute lysine residues in histone H3, individually or in combinat...

nature.com

Our latest: Minimal essential requirements for neural tube self-organisation How does a single cell give rise to a tissue with the right cell types in the right proportions? We deconstruct and rebuild a self-organising tissue from first principles A thread🧵 www.biorxiv.org/content/10.6...

Minimal essential requirements for neural tube self-organisation

The reliable generation of diverse cell types in precise proportions is essential for the formation of functional tissues during embryonic development. Three-dimensional organoid models derived from p...

biorxiv.org

Online Now! Signaling reprogramming via Stat3 activation unravels high-fidelity human post-implantation embryo modeling #stemcells

Signaling reprogramming via Stat3 activation unravels high-fidelity human post-implantation embryo modeling

Enhanced STAT3 activation combined with TGF-β inhibition reprograms human PSCs into cells specifying all early embryonic lineages. Upon 3D suspension culture, these cells self-organize within 6 days into post-implantation structures mimicking Carnegie stages 5–7 embryos, including gastrulation, with high morphological and molecular fidelity.

dlvr.it

(1/8) Very Happy to share our lab’s 3D post-implantation human embryo model, made via signalling reprograming! 🙌 Huge thanks to the entire team and our collaborators, special shout-out to @cc0447.bsky.social (led the work) and @jinjin-0101.bsky.social (comp. analysis). www.cell.com/cell-stem-ce...

Signaling reprogramming via Stat3 activation unravels high-fidelity human post-implantation embryo modeling

Enhanced STAT3 activation combined with TGF-β inhibition reprograms human PSCs into cells specifying all early embryonic lineages. Upon 3D suspension culture, these cells self-organize within 6 days i...

cell.com

(1/3) A while back, my then-PhD student @htstuart.bsky.social stumbled on a finding that changed our lab’s direction 👀✨. Turns out, STAT3 mediated reprogramming generates intermediates (Gata6GFP+ cells) going through an earlier developmental stage with increased developmental potential 🚀

💓 Researchers in the @josesilvalab.bsky.social used #SmallMolecules to convert mouse ESCs into embryo founder cells, which generated a complete embryo model! Article: bit.ly/4orpbLb Episode with discussion: bit.ly/4oTURt2

🥂Congrats to Gao, Li & co for their new @natcellbio.nature.com study: they derive #trophectoderm stem cells from 32-cell mouse #embryos. These cells represent an early trophectoderm state, develop into #placenta cells and organoids and contribute to blastoid generation. rdcu.be/eAKzp bit.ly/4fyZ9Sg

Mouse trophectoderm stem cells generated with morula signalling inducers capture an early trophectoderm state - Nature Cell Biology

Gao, Li and colleagues derive trophectoderm stem cells from 32-cell mouse embryos. These cells represent an early trophectoderm state and are capable of developing into placenta cells, forming placent...

bit.ly

Capturing early TE stem cells. From Zhang Jian’s lab, “Mouse trophectoderm stem cells generated with morula signalling inducers capture an early trophectoderm state”, with a small contribution from us. www.nature.com/articles/s41...

Mouse trophectoderm stem cells generated with morula signalling inducers capture an early trophectoderm state - Nature Cell Biology

Gao, Li and colleagues derive trophectoderm stem cells from 32-cell mouse embryos. These cells represent an early trophectoderm state and are capable of developing into placenta cells, forming placent...

nature.com

Very happy to share the work of Huanhuan Li et al. While not a natural embryo, you might not have known that if I hadn’t told you. (1/3)

Join the VGZT Eastern KEYNOTE lecture ☀️☕ TOMORROW 🗓️ Wednesday July 09 ⏰ 13:30 IST / 17:00 JST / 8:00 UTC / 10:00 CET Featuring: 1) Maithreyi Narasimha 👉Positioning tissues during Drosophila morphogenesis Jose Silva @josesilvalab.bsky.social 👉From founder cells to organogenesis-stage embryo model

Thank you VGZT community for your active participation 🌎 See below the session flyers for the rest of the season: 🌞 Eastern sessions: Happening on Wednesdays. Our keynote for this season: Maithreyi Narasimha and @josesilvalab.bsky.social [1/2]

Very grateful to the @lundstem.bsky.social and Filipe Pereira for hosting me. I truly enjoyed meeting and discussing with many colleagues at the Center. I also received very interesting feedback that will hopefully lead to collaborations and help further improve our embryo models.

Lund Stem Cell Center@lundstem.bsky.social · last yr.

💡✨Can reprogramming stem cells into "embryo founder-like cells" advance their use in the clinic? Yesterday, José Silva from Guangzhou National Laboratory joined our Stem Cell Stars Seminar to explore this. #StemCells Thanks @josesilvalab.bsky.social for great discussions, insights & connections! 👏

An interesting study characterising human embryo gastrulation! rdcu.be/d5VmN

Spatial transcriptomic characterization of a Carnegie stage 7 human embryo

Nature Cell Biology - Guo and colleagues characterize an intact Carnegie stage 7 human embryo at single-cell resolution in a spatially resolved manner.

rdcu.be