Do any of the CryoET national centers have SPACEtomo set up for users? I've got several montaging and/or bigsmall projects I'd like to try but we have no support for either at PNCC. #teamtomo

One cell, one tomogram! Our montage tomography workflow, MOSAIC, is out in Structure, and MontageMaker is on GitHub! MOSAIC: www.cell.com/structure/fu... MontageMaker: github.com/MPI-Dortmund... Big thanks to @maikaboiero.bsky.social, Adriana Prajica, Gavin Rice, and @raunser-lab.bsky.social!

In #cryoET, separating closely apposed membrane segmentations into distinct labels can be difficult. Small artefacts may connect otherwise separate membranes, requiring tedious slice-by-slice correction. To address this, @jasmred.bsky.social and I developed MemSplit, a napari based workflow.

Pepper learned how to get on top of the refrigerator this morning. #blackcat

I am currently working through a STOPGAP tutorial for #CryoET. I am using UCSF Chimera to pick the particles now and I get this warning about the impending loss of support in future macOS versions.

Cryo-ET is often framed as a tool for in situ protein structure. But what if the real revolution is contextualization? I explore how #teamtomo is redefining what "local" means in organelle biology, revealing membrane states rather than just protein structures. tinyurl.com/localmembrane

Think globally, act locally: Redefining organellar membrane environments through cryo-electron tomography

Early enthusiasm for the “cellular revolution” in cryo-electron tomography (cryo-ET) was largely driven by the promise of resolving protein structures…

sciencedirect.com

Very excited about our new work highlighting the role of lipids in mediating multi-channel assemblies of Cx-46/50!

Reichow Lab@reichowlab.bsky.social · 2mo ago

Dual-channel Cx46/50 gap junction assemblies in a shared lipid-nanodisc = lipid-mediated packing and a lipid site near the NT gating region that is absent from single channels. Plus a 1.8 Å open-state single-channel benchmark! #cryo-EM 🍩’s #NIH-funded-research www.biorxiv.org/content/10.6...

Excited to share our work on the structure and function of cytoplasmic lattices within mouse embryos. A collaborative effort with @niakanlab.bsky.social and work led by @kashishsingh.bsky.social and @inaharasimov.bsky.social . It is now out on BioRxiv: www.biorxiv.org/content/10.6...

New Title Alert: easymode- a collection of general pretrained networks for cellular cryoET. Learn more here: buff.ly/mLbcUbZ #SBGrid #SBGridSoftware

easymode

buff.ly

It's Friday the 13th, which means it is a black cat appreciation day! #BlackCat

Just here to pay my regular #CatTax by sharing some photos of Pepper from this past week. #CatsofBlueSky

Enjoying a work from home day with Pepper. #Cat #Cattree

Happy #Caturday.

Got Pepper's collar back on him last night. Snapped this photo as he played with his toy. Only time will tell if the collar stays on! #NewCoverImage #CatsofBluesky

I've been working on Surface Morphometrics for a new version (coming soon), and made some significant (AI-assisted) performance improvements, especially in pycurv (20-50x!). If you've had technical issues or just felt like it was too slow, give it another try! #teamtomo github.com/GrotjahnLab/...

GitHub - GrotjahnLab/surface_morphometrics: Morphometrics for Membrane Surfaces Segmented from Cryo-ET or other volumetric imaging.

Morphometrics for Membrane Surfaces Segmented from Cryo-ET or other volumetric imaging. - GrotjahnLab/surface_morphometrics

github.com

My buddy. #Cats

New mug from my mother to commemorate my becoming a cat dad. #catdad #coffee

Our paper is finally out! I’m proud to share this collaborative work using FIB milling and cryo-electron tomography to study tick-borne flavivirus replication. Thanks to all co-authors and collaborators — great teamwork #CryoET #ElectronTomography #Virology #Flavivirus #Microscopy #StructuralBiology

Lars-Anders Carlson@lacarlson.bsky.social · 6mo ago

I am super happy to share this paper in its final form. We used FIB milling to "dig in to" cell lines and mouse brains infected with tick-borne flaviviruses, followed by cryo-ET to study the virus replication. It's open access, so have a look! #virology #teamtomo www.nature.com/articles/s41...

It’s been an adventure this weekend. On Saturday I adopted this little guy at the Oregon Humane Society and have been working on getting him settled. He is shy, but he has a very loud purr when he gets his neck pets and scratches. He also loves Churus! Say hello to Pepper! #Cats #SpecialRescue

#teamtomo gets another quantitative cell biology tool! We extend our Surface Morphometrics pipeline to measure membrane thickness. Alongside many biological findings, we show that our 3D measurements closely match in vitro vesicle measurements from 2D EM (collab w/Neal Waxham and Fred Heberle).

Journal of Cell Biology@jcb.org · 7mo ago

Medina, Chang et al. of the @nanigrotjahn.bsky.social lab introduce a new method based on the Surface Morphometrics pipeline to measure global and local thicknesses of organellar #membranes from cellular cryo-electron tomography data. rupress.org/jcb/article/... #CryoET

By leveraging in situ cryo-ET, Tian et al. obtained high-resolution, #3D views of the native #ultrastructure of intact hippocampal #synapses. See here: doi.org/10.1007/s122...

cryoEM papers@cryoempapers.bsky.social · 7mo ago

Subcellular Structures in Native Hippocampal Synapses Revealed by Cryo-electron Tomography pubmed.ncbi.nlm.nih.gov/41491937/ #cryoEM

Happy new year #teamtomo! We've resumed our OHSU tomo journal club, and this year I am going to be sharing it on bluesky. This week we did a roundtable, where attendees each brought a paper and did a 3-5 minute discussion of what they thought was cool about it. Here are the papers they discussed!

Online today, the journal version of our ( @nanigrotjahn.bsky.social @attychang.bsky.social @mmedina300kv.bsky.social) manuscript from earlier this year describing membrane thickness measurement in cells with #teamtomo! rupress.org/jcb/article/...

May your holidays have the joy of a cat in an empty box on Christmas. #HappyHolidays #Christmas

In the Ring: Details of the bacterial cell division structure the Z-ring revealed using cryo-ET 📷 Adrián Merino-Salomón et al @mpibiochem.bsky.social in @elife.bsky.social ➡️ bpod.org.uk/archive/2025... with John Ankers

I am so grateful Apple brought Preview to the iPad on OS 26. It has made being without my MacBook for the week so much easier. The iPad is still not a laptop replacement, but it has gotten much closer to that recently.

Excited to share our preprint on cytoskeletal organization in astrocytes! biorxiv.org/content/10.110… Postdoc @mewynne759 looked at microtubules, IFs/GFAP & actin along long elaborate processes. Wonderful to collaborate w postdoc @dharshinigopal & @NogalesLab on cryo-ET 🤓 1/x

Check out our preprint! With new molecular mechanisms, 140 subtomogram averages, and ~600 annotated cells under different conditions, we @embl.org were able to describe bacterial populations with in-cell #cryoET. And there’s a surprise at the end 🕵️ www.biorxiv.org/content/10.1... #teamtomo

Meanwhile in “war ravaged Portland.” #PDX

Woo! My lab's Nature Protocols paper is out today. In a nutshell, the protocol shows how to use our Cryo-ET software to solve high-res protein structures. I don't think any of the other authors are here, but congrats to Hsuan-Fu, Ye, and Wendy for all of their work! www.nature.com/articles/s41...

In situ structure determination of conformationally flexible targets using nextPYP - Nature Protocols

The procedure for nextPYP facilitates the end-to-end processing of cryoelectron tomography data for high-resolution structure determination and conformational variability analysis.

nature.com