We love using mNeonGreen for live but have failed to find good reagents for things like expansion microscopy. This is a fantastic addition to the toolbox. Great work!

Proud of our team and grateful to everyone who contributed to this journey. This paper reflects our shared passion for science and our commitment to developing reliable research tools for the scientific community. 🦙💚🔬

A High-Affinity Nanobody Recognizing mNeonGreen Enables Versatile Biochemical, Cellular, and in vivo Applications. by Steffen Frey and team: www.biorxiv.org/content/10.6...

New paper! 🚀 A high-affinity anti- #mNeonGreen #nanobody turns #mNG into a #multifunctional Tag. @nanotag.bsky.social decided to openly share the #nanobody for research use. Not common, but we strongly believe well-defined reagents are key for reproducible science. We hope you find it useful! 💚

8 hours. That’s all fish need to heal wounds 🐟⏱️ Why are they so fast compared to humans? I break down the science of rapid regeneration in a microscopy story 🔬 Watch here: youtu.be/Mt9f61adaP0 #FluorescenceFriday #Microscopy

Our new paper in @plosbiology.org introduces IntAct-U-ExM enabling isoform-specific imaging of actin at nanoscale across yeast, mammalian cells & primary neurons. Fabulous work spearheaded by @anubhavdhar.bsky.social along with an amazing team! #actin #U-ExM journals.plos.org/plosbiology/...

Make HDAC6 visible again! 6SiR-C3-NextA is a selective far-red probe for imaging endogenous HDAC6 in living cells across diverse mammalian cell types. Done by @vanthang.bsky.social at @mpi-nat.bsky.social Now in JACS @pubs.acs.org pubs.acs.org/doi/10.1021/...

Resolution isn't just optics. Structural resolvability also depends on probe size, linkage error, labelling efficiency, steric hindrance and, molecular organisation. VLab4Mic models all of these starting from PDB or AlphaFold structures

It is finally out there in published form. 🎉 #MicroSplit (with an 𝕊) #Microscopy 🔬 #AI 🤖 www.nature.com/articles/s41... And no paywall... so... enjoy the read! 💸 👋 This is where the post could end... but I will have to go on for a bit below... 😉 🧵

New paper out! Left-handed DNA cannot bind to natural, right-handed DNA. This leads to lower background if it is used as a probe in DNA-PAINT. Using Fluorigenic DNA-PAINT with left-handed DNA allows for low background, fast 3D DNA-PAINT! Congrats, Bas! skeetorial: 1/x pubs.acs.org/doi/full/10....

Accelerated Left-Handed DNA-PAINT Using Fluorogenic Probes

Single-molecule localization microscopy (SMLM) techniques offer nanometer-scale resolution by stochastically localizing individual fluorescent molecules. Among these, DNA-mediated point accumulation f...

pubs.acs.org

There is still time to apply. I'm now looking for someone who likes imaging, for a project like this: www.cell.com/neuron/fullt...

Subhojit Roy MD, PhD@roylab-ucsd.bsky.social · 4mo ago

Our lab has an open postdoctoral position. Work in the lab ranges from trafficking of neuronal cargoes to developing gene editing therapeutics for Alzheimer's and Parkinson's. I'm looking for the right person, project is flexible. Please apply immediately. www.roylab.org

📣 Neuroscientists – something NEW for you! NanoTag expands its portfolio of neurobiological #rHcAbs 🧠. Nanobodies targeting #SYT1 and #CNPase are now available as Mouse or Rabbit IgG Fc fusions – ideal for ICC and IHC 🔬. Learn more 👉 bit.ly/4cKITOd #Neuroscience #RecombinantAntibody

🚀 Just published! In PhD work by first author Dongchen Du, we’ve developed a simple, byproduct-free labeling strategy using imidazopyridinium dyes formed in situ from isoquinolines! onlinelibrary.wiley.com/doi/full/10.... #Fluorescence #Bioconjugation #ChemicalBiology #InSituLabeling

In Situ Construction of Imidazopyridinium Fluorescent Labels for Bioconjugation

Chromophore engineering of isoquinoline aldehydes generates TICT-based fluorogenic dyes for in situ labeling of diverse (bio)molecules with large Stokes shifts compatible with cell imaging.

onlinelibrary.wiley.com

New preprint out! In this study, we introduce NanoFLex, a strategy that combines #HaloTag variants with #nanobodies -based immunolabeling to enable rapid, OneStep-IF #lifetime #multiplexing. Possible due to SmartSecondaries fused to #HaloTag from @nanotag.bsky.social: www.biorxiv.org/content/10.6...

Delighted to share our latest preprint "Filopodia-mediated trans-endocytosis" www.biorxiv.org/content/10.6... Work driven by many people including Hanna, Marcela, Sujan, Marie-Catherine, Anna and Monika. Fantastic collaboration with Johanna Englund, Emilia Peuhu, and Eija Jokitalo

🧪🧬 New preprint! What if the same biosensor could reveal how cysteine is regulated across life domains? We introduce Cystector, a genetically encoded fluorescent biosensor for real-time cysteine monitoring in living cells. www.biorxiv.org/content/10.6... 🧵A thread ⬇️

Last project of the year: self blinking JF635 + lattice light sheet + #BigVolumeBrowser to deskew both volume and localization data (and render) @zeiss-microscopy.bsky.social

Felipe Opazo@opazo.bsky.social · 8mo ago

✨ Blinking #nanobodies that work for single-molecule localization 🔬 Our new preprint shows that the self-blinking dye JF635b restores robust, buffer-free blinking in #nanobodies, enabling reliable #dSTORM, #MINFLUX, and more, without chemical-switching buffers. Opening new possibilities for #ExM!

We also tried it recently and indeed, it blinks nicely without any smelly buffers or extra stuff added. Makes SMLM sample preparation a breeze. I hope soon new additional colors will be discovered, apart from the far red.

Felipe Opazo@opazo.bsky.social · 8mo ago

✨ Blinking #nanobodies that work for single-molecule localization 🔬 Our new preprint shows that the self-blinking dye JF635b restores robust, buffer-free blinking in #nanobodies, enabling reliable #dSTORM, #MINFLUX, and more, without chemical-switching buffers. Opening new possibilities for #ExM!

🎄 A glimpse into #NanoTag Christmas dinner - Glühwein outdoors, a shared dinner, and Schrottwichteln 🎁♻️ where funny, used items are exchanged and find a new home. The past year has been special for us as we celebrate 10 years. Grateful for our team, customers, and partners. Here’s to what’s next 🥂

✨ Blinking #nanobodies that work for single-molecule localization 🔬 Our new preprint shows that the self-blinking dye JF635b restores robust, buffer-free blinking in #nanobodies, enabling reliable #dSTORM, #MINFLUX, and more, without chemical-switching buffers. Opening new possibilities for #ExM!

Happy to tell that I got my tenured professorship at the University Medical Center in Göttingen 🇩🇪! Grateful for my students, mentors, international collaborators, the @nanotag.bsky.social team, and my family (dog included 🐾). Motivated to keep pushing science, innovation, startups, and education 🌍✨

#NanoTag joins the Life Science Ecosystem at Nordic Life Science Days #NLSDays2025 in Gothenburg!🇸🇪 Meet our CEO Hansjörg Götzke at the Booth B03:46 (German Pavilion), and discover our sdAb platforms🦙, protein production services, and research tools🔬advancing #LifeScience & #Biomedicine.

Fantastic talk of @ewerslab.bsky.social with ExM data on Septins and Unroof Cells in #GEF25!

🦙 NanoTag at #SingleDomainAntibodies Conference 2025! Our CEO Hansjörg Götzke will be in Utrecht sharing how our immune & synthetic sdAb platforms drive advances in research, diagnostics & therapeutics. Our founder Felipe Opazo @opazo.bsky.social will also be there to connect & share insights.

#ExM turned 10 this year - just like #NanoTag! 🎉 Join us & the super-resolution community in Göttingen for the ExM Forum! 🔬Hear from our founder Felipe Opazo @opazo.bsky.social and CSO Steffen Frey on how NanoTag’s sdAb solutions empower nanoscopy & #multiplex imaging. See you in Göttingen! #GEF25

Prefusion gB of HSV bound with a neutralizing nanobody www.nature.com/articles/s41...

A nanobody specific to prefusion glycoprotein B neutralizes HSV-1 and HSV-2 - Nature

A neutralizing nanobody specific to the prefusion conformation of herpes simplex virus glycoprotein B has cross-species activity and offers insights into virus neutralization, possible immunogens and ...

nature.com

🔬Wrapping up #SMLMS2025 @smlm-symposium.bsky.social in Bonn! Thanks to everyone who stopped by our booth! It was great connecting and sharing how our #sdAb-based solutions enhance precision, simplify #Multiplexing, and streamline workflows in #SuperResolution microscopy. See you next year! 🦙

Out today in @nature.com: Together with the Honigmann, Shevchenko, Drobot and Hof labs, we present a general workflow for imaging the localization and transport of individual lipids in cells and mapping their metabolism. www.nature.com/articles/s41...

🧬 New paper out! We explored why #antibodies have kept their 2 binding sites. Turns out, this “divalence” is crucial for B-cell receptors to sense antigen clustering and trigger immune responses, especially for weak or low-density antigens. Check full text: doi.org/10.1038/s414...

- A postdoc to work on cancer extravasation (4-year project). - A postdoc help in completing Filopodia-related lab projects (2-year). - Multiple Marie Curie postdoc positions that will open in the fall. - We will open a 5-year position to cover my teaching (70% research and 30% teaching).

Guillaume Jacquemet@guijacquemet.bsky.social · last yr.

Are you looking for a postdoc? I will be looking for 2-4 people after the summer to join the lab !! Do not hesitate to reach out and disseminate !!