📢New paper in @journal-evo.bsky.social! This work expands our understanding of seasonal camouflage polymorphism, dissecting the genetic basis of winter colour variation (white and brown morphs) in the long-tailed weasel (Neogale frenata). Paper here ⬇️ doi.org/10.1093/evol... 1/5
Museum genomics links MC1R alleles to adaptive winter coat color polymorphism in the long-tailed weasel
Abstract. Understanding the architecture of biological adaptations is a major endeavor of evolutionary biology. Using Natural History collections, we study
doi.org
![Fig. 1 of Meyer et al. Summary of the experimental workflow. Seven-day-old Vicia faba seedlings were transferred to hydroponics and grown for 4 weeks before treatment. Direct alkalinisation of leaf apolplastic pH (pHapo) was achieved via infiltration with 20 mM MOPSO buffered at either pH 4.25 or 6.5, with pure water used as a control; imaging began immediately after treatment. Salinity-induced alkalinisation of pHapo was achieved by adding 100 mM NaCl to the roots; imaging continued for a further 160 min. Ratiometric imaging was conducted using a fluorescence microscopy system and the pH-sensitive dye Oregon Green 488. Immediately after the end of imaging the samples were frozen in liquid nitrogen for subsequent sample preparation and analysis, as shown. The post-hoc analyses utilised the parameters AUC [H+]apo and Δmax [H+]apo that were derived from the pHapo kinetics of individual plants, and a partial least-square component (PLS 1; see Methods).](https://cdn.bsky.app/img/feed_thumbnail/plain/did:plc:ggz22gcwkakll7efac23g33s/bafkreibbv6mjinjcg7gbew72xhewmqgd23sdygc2jls7rprg6rgygk3kry)
