Constantin Anoyatis
@canopele.bsky.social
Posdoctoral researcher, Bacterial Genome Plasticity, Institut Pasteur Former peptidoglycan enthousiast in the Hugonnet Lab in Paris From autolysins and β-lactams to replicon domestication🧫
Yesha Patel here reviews how mutations in RNAP contribute to antibiotic resistance, with examples from both laboratory evolution and clinical settings. www.sciencedirect.com/science/arti... This work builds on two of her prior papers (PMIDs: 36779708 and 39908144). #MicroSky #antibiotics
sciencedirect.com
Cell-to-cell cooperation through membrane vesicles enhances antibiotic persistence @science.org @bcmfromthelabs.bsky.social www.science.org/doi/10.1126/... www.science.org/doi/10.1126/...
The preprint of my PhD paper is out ! We show cooperativity between various mobile elements which exploit the Xer machinery for lysogeny in Vibrio cholerae. Among them, the CTX bacteriophage, responsible toxigenic conversion. www.biorxiv.org/content/10.6...
Molecular interplay between Integrative Mobile Elements exploiting Xer drives the evolution of cholera pandemics
XerC and XerD are ubiquitous bacterial recombinases dedicated to chromosome dimer resolution. They add a crossover at a specific chromosomal site, dif, when activated by the FtsK cell division protein...
biorxiv.org
👉 (p)ppGpp: the magic goes on! | Microbiology and Molecular Biology Reviews journals.asm.org/doi/10.1128/...
(p)ppGpp: the magic goes on | Microbiology and Molecular Biology Reviews
SUMMARYBacteria are constantly exposed to changing environmental conditions, and to survive they need to adapt quickly, adjusting their gene expression and metabolism to make the most of the resources...
journals.asm.org
This one means a lot. Our paper, “Large extrachromosomal replicons are widespread across bacterial lineages and show coordinated replication termination and spatial coupling with the chromosome,” is out! doi.org/10.1038/s414...
Large extrachromosomal replicons are widespread across bacterial lineages and show coordinated replication termination and spatial coupling with the chromosome - Nature Communications
Bacterial genomes often include extrachromosomal replicons, ranging from small plasmids to chromosome-scale replicons. Here, Czarnecki et al. analyse more than 40,000 complete bacterial genomes and sh...
doi.org
Delighted to share a new paper from our lab, a study led by Mathieu Bergé looking at how an endogenous toxin targets replication to induce competence in the pneumococcus. dx.plos.org/10.1371/jour...
A toxin/antitoxin system targeting the replication sliding-clamp induces competence in Streptococcus pneumoniae
Author summary The environment in which bacteria live puts them under a great deal of stress, forcing them to adapt constantly, either temporarily or permanently. Streptococcus pneumoniae, a pathogeni...
dx.plos.org
Our new paper on DusB’s role in redox metabolism in V. cholerae is now out in @narjournal.bsky.social linking tRNAmodif enzymes and metabolic adaptation. Beyond RNA modification: a tRNA-modifying enzyme shaping oxidative stress resilience and metabolism in V. cholerae #rnasky #rnabiology 😊🦠💫
Beyond RNA modification: a novel role for tRNA modifying enzyme in oxidative stress response and metabolism
Abstract. RNA modifications play a fundamental role in regulating essential cellular processes, including translation fidelity and stress adaptation. While
academic.oup.com
What is better than one? Two connected papers! #NewResearch S protein of Streptococcus pneumoniae activates PBP1a and coordinates with a wider GpsB-associated multi-protein complex to regulate peptidoglycan remodelling and cell division. #MicroSky 🦠 www.nature.com/articles/s41...
Streptococcus pneumoniae S protein activates PBP1a to regulate peptidoglycan remodelling and cell division - Nature Microbiology
S protein interacts with and activates PBP1a during cell division, as part of a larger GpsB-associated multiprotein complex that coordinates peptidoglycan remodelling in Streptococcus pneumoniae.
nature.com
Nature Microbiology@natmicrobiol.nature.com · 9mo ago#NewResearch S protein from Streptococcus pneumoniae activates cell wall repair and modification to promote virulence against in vivo antimicrobial pressures including lysozyme and LL-37 @veeninglab.com #MicroSky 🦠 www.nature.com/articles/s41...
New online: AlphaSync is an enhanced AlphaFold structure database synchronized with UniProt
AlphaSync is an enhanced AlphaFold structure database synchronized with UniProt
Nature Structural & Molecular Biology, Published online: 11 November 2025; doi:10.1038/s41594-025-01719-xAlphaSync synchronizes AlphaFold Protein Structure Database predictions with UniProt sequences, providing up-to-date protein structures with residue-level annotations for humans, model organisms and pathogens through an accessible web interface and application programming interface.
go.nature.com
The stringent response does not influence ribosome pausing in Bacillus subtilis @narjournal.bsky.social from Leendert Hamoen academic.oup.com/nar/article/...
The stringent response does not influence ribosome pausing in Bacillus subtilis
Abstract. The stringent response represses translation and is activated when cells enter the stationary phase and intracellular amino acid levels drop. Bac
academic.oup.com
🦠🔬🤖🧑💻 #mAIcrobe is out! With @pinholab.bsky.social's lab, we launched an open-source framework for high-throughput bacterial image analysis. By rockstars A. Brito & B. Saraiva et al, making #DeepLearning for phenotyping accessible! Easy to use, plus model training 📜 www.biorxiv.org/content/10.1...
A global view of morphogenetic peptidoglycan synthases across the domain Bacteria Open Access @femsjournals.bsky.social microLife by Francisco García-del Portillo et al academic.oup.com/microlife/ad...
A global view of morphogenetic peptidoglycan synthases across the domain Bacteria
Abstract. Bacteria define their heritable cell shape using membrane integral glycosyltransferases (GTases) of the shape, elongation, division and sporulati
academic.oup.com
This work is finally published! 🥳🧬 Plasmids are associated with very variable fitness costs in their different bacterial hosts. But, what is the contribution of each of the plasmid-genes in these host-specific effects? Study led by @jorgesastred.bsky.social, @sanmillan.bsky.social and myself! 1/14
Dissecting pOXA-48 fitness effects in clinical Enterobacterales using plasmid-wide CRISPRi screens
Nature Communications - This study investigates the effects of the carbapenem resistance plasmid pOXA-48 in clinical enterobacteria. Using CRISPRi screens, the authors revealed that the...
rdcu.be
Our paper on the Bacteroidota BAM complex is out in @natmicrobiol.nature.com! With @madejmar.bsky.social We found that BAM in Bacteroides and Porphyromonas gingivalis has a distinct architecture from BAM in Proteobacteria. doi.org/10.1038/s415...
Structure of a distinct β-barrel assembly machinery complex in the Bacteroidota - Nature Microbiology
Structural and functional characterization of the β-barrel assembly machinery complex in Bacteroidota reveals a distinct, seven-component complex with a large extracellular domain that may enable β-barrel–surface lipoprotein complex assembly.
doi.org
Proud of our new study out in @cp-cellreports.bsky.social! Using E. coli lacking all 8 endopeptidases, we provide direct evidence that peptidoglycan expansion during elongation requires ED-mediated insertion of one glycan strand at a time. This can be performed by MepS, MepM, MepH and PBP7 #microsky
Role of endopeptidases in lateral cell wall expansion in Escherichia coli
Peptidoglycan, the major constituent of bacterial cell walls, is a giant macromolecule made of glycan strands cross-linked by short peptides, which pr…
sciencedirect.com
(p)ppGpp-dependent activation of gene expression during nutrient limitation #mBio from Jonathan Dworkin journals.asm.org/doi/10.1128/...
(p)ppGpp-dependent activation of gene expression during nutrient limitation | mBio
Bacteria often experience nutrient limitation and, in response, attenuate energetically costly metabolic processes like protein synthesis. At the same time, however, they stimulate the expression of a subset of proteins that facilitate survival under ...
journals.asm.org
Congrats @herailquentin.bsky.social
Hugonnet Lab@jeh-lab.bsky.social · last yr.Thrilled to announce our new paper with M. Ethève-Quelquejeu's lab @upcite.bsky.social published @chemistryeurope.bsky.social! We report on the synthesis, and testing by HRMS, of new β-lactam prodrugs, a proof of concept for designing drugs targetting specifically β-lactamase-producing pathogens.
Excited to present our new study on the mechanism of lateral peptidoglycan expansion in B. subtilis. We combined microscopy visualisation with clickable D-Ala-D-Ala analogues and stable-isotope peptidoglycan labelling to describe an inside-to-outside expansion model @natcomms.nature.com.
Mechanism of lateral cell-wall expansion at a constant diameter in Bacillus subtilis
Nature Communications - In Escherichia coli, lateral cell-wall expansion during growth occurs by crosslinking of new glycan chains to the existing peptidoglycan network. Here, Liang et al. show a...
rdcu.be
Very excited to share our work on (p)ppGpp-mediated resistance to beta-lactam antibiotics published in Nature Microbiology!! A great outcome for a project we started working on 5 years ago. A big thank you to all authors that participated in this study! www.nature.com/articles/s41...
(p)ppGpp modifies RNAP function to confer β-lactam resistance in a peptidoglycan-independent manner - Nature Microbiology
The bacterial alarmone (p)ppGpp induces β-lactam resistance through modification of RNA polymerase and ribosome function rather than regulation of peptidoglycan metabolism in Escherichia coli.
nature.com