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Colin Conine

@colinconine.bsky.social

Small RNA biologist at UPenn & CHOP www.coninelab.com Interested in small RNAs, germlines, inheritance, sports, and Shiba Inu’s

56 Posts718 Follower499 Folgt
James Gagnon@james-gagnon.bsky.social · 2d ago

In a new (ish) preprint, we used CRISPR barcodes to label germline stem cell clones in zebrafish, then tracked their contributions to sperm production for 2 years. Instead of staying stable, individual clones rose, fell, and disappeared over time. www.biorxiv.org/content/10.6...

Clonal dynamics deviate from neutral drift in zebrafish spermatogenesis

Spermatogonial stem cells (SSCs) maintain male fertility, but how their clonal dynamics change with age remains poorly understood. Here, we use in vivo CRISPR barcoding in zebrafish to label SSCs and ...

biorxiv.org

Jay Shendure@jshendure.bsky.social · 5d ago

Thrilled to post thread re: new single-cell lineage of mouse embryo reconstructed w/ DNA Typewriter. One animal, zygote to late organogenesis (E13.5). Tree has 1,340,794 transcriptionally profiled, annotated tips (cells), 1,142,588 dated internal nodes, rooted at zygote 1/n

Alejandro Montenegro@aemonten.bsky.social · 7d ago

"These findings provide a framework for understanding the role of (...) secreted miRNAs in inter-organ communication." www.nature.com/articles/s42...

Brown fat-specific RNA labelling reveals a network of inter-organ communication by secreted microRNAs - Nature Metabolism

This study uses in vivo, 4TU-based, tissue-specific miRNA labelling to highlight the endocrine role of BAT-derived miRNAs that are taken up by tissues like the muscle, liver and hypothalamus, and demo...

nature.com

Turner Lab@lab-turner.bsky.social · 4w ago

New preprint 📢 Thrilled to share our long-awaited work on the role of RSX in marsupial X-inactivation. This was a huge team effort over the years, led by @aureliencourtois.bsky.social @sermenchero.bsky.social Sugako Ogushi and Waz Varsally, that allowed us to set up genome editing in opossum embryos

Genome editing of marsupial RSX reveals conserved and divergent principles in mammalian X-inactivation

X-chromosome inactivation balances X-gene dosage between the sexes in eutherians and marsupials. The lncRNA Xist mediates X-inactivation in eutherians but is absent from marsupials. An evolutionarily ...

biorxiv.org

Cohen Lab @Cornell@cohenlab.bsky.social · last mo.

very proud to announce the publication of our newest work with Andrew Grimson demonstrating non-canonical roles for AGO3 and AGO4 in mammalian meiosis. Bravo to @mercedes-carro.bsky.social who led this work fearlessly and relentlessly, from mass spec to multiomics. journals.plos.org/plosgenetics...

Argonaute proteins orchestrate Meiotic Sex Chromosome Inactivation and timing of the spermatogenic transcriptional program

Author summary In order to make spermatozoa, germ cells undergo the meiotic specialized cell division, during which the sex chromosomes are temporarily shut down in a process called Meiotic Sex Chromo...

journals.plos.org

Jason Shepherd@jasonsynaptic.bsky.social · last mo.

Very excited for our new paper, now out in @cellcellpress.bsky.social! /🧵 The protein Tau forms intracellular toxic tangles in neurons in Alzheimer’s disease and Tauopathies. Tau pathology slowly spreads from cell-to-cell but the mechanisms are not clear /1 authors.elsevier.com/sd/article/S...

Arc mediates intercellular tau transmission via extracellular vesicles

Tau pathology spreads cell to cell, but the mechanisms of intercellular tau transmission remain unclear. We find that the neuronal gene Arc is critica…

authors.elsevier.com

Harmit Singh Malik@harmitmalik.bsky.social · last mo.

I know it seems like a heretical idea, but one way to figure out if a preprint is really interesting (to you) is... to read the preprint. Then, in the spirit of sharing, send a note to authors: a simple kudos or a more detailed question. I do this a dozen times a year. It's awesome. Try it.

Knowable Magazine@knowablemag.bsky.social · 2mo ago

Researchers think that factors such as paternal diet, smoking, exercise and exposure to poisons and pollutants may exert an effect, via sperm, on the next generation. #FathersDay ✍️ Holly Barker: knowmag.org/4eo6Ief

Do you take after your dad’s RNA?

Evidence is growing from rodent studies that sperm carries marks of a father’s life experiences, influencing traits in offspring. Bits of RNA could be the drivers of these epigenetic effects.

knowmag.org

Christine Mayr@christinemayr.bsky.social · 2mo ago

Finally out in @Cellcellpress! Proteins with long IDRs are prone to misfolding during protein synthesis. This is prevented by mRNA 3′UTRs that act as mRNA-based IDR chaperones. www.cell.com/cell/fulltex...

mRNA 3′ UTRs chaperone intrinsically disordered regions to control protein activity

Highly conserved mRNA 3′ UTRs act as co-translational chaperones for intrinsically disordered regions (IDRs), preventing inter-domain misfolding and enabling biogenesis of fully active proteins.

cell.com

Jennifer O’Chan@chanjenerational.bsky.social · 3mo ago

Thrilled to share my main postdoctoral work out @nature.com ! This project tackles a fundamental question: how do pregnancy and postpartum experiences promote lifelong changes to the brain? www.nature.com/articles/s41...

Dopamine drives persistent remodelling of the maternal brain - Nature

Brain-wide transcriptomic profiling in mice reveals that reproductive experience remodels the maternal brain by altering dopamine dynamics in the dorsal hippocampal formation, causing dopamine-depende...

nature.com

Ollie Rando@epididymosome.bsky.social · 3mo ago

Hey, wouldja look at that! An incredible one man show from Pablo Bora, an amazing postdoc...we used Steven Floor's beautiful REMORA approach (fusing RNA base editors to RBPs, allowing RBP cargo to be identified by edits) to explore RBP-RNA binding in mouse embryos doi: doi.org/10.64898/202...

Probing RNA-protein interactions in the early mouse embryo

The union of two germ cells to form a zygote, and subsequent early embryo development, are marked by radical remodeling of virtually every major class of biomolecules as the specialized germline states give way to the rapid and active growth that marks early development. In recent years, advances in ultra-low input genome-wide methods have enabled systematic analyses of mRNA abundance, and of chromatin organization, throughout early development in a variety of model systems. Here, we extend these efforts to the study of RNA binding protein (RBP) function in early mouse embryos, adapting REMORA 1 -- based on fusing an RNA-editing enzyme to an RBP of interest -- for use in early embryos. We benchmark our approach for several well-studied RBPs, successfully recovering expected features of their RNA cargos, and assayed the RNA cargos for 17 RBPs of interest for early gene regulation. Analysis of changes in mRNA metabolism following knockdowns of the RBPs surveyed here allowed us to identify direct regulatory functions for a subset of RBPs in the early mammalian embryo, including an unanticipated role for the RNA export adaptor Alyref in control of 3 prime polyadenylation sites. Together, our data provide a proof of concept resource for systematically exploring RBP functions in mammalian embryogenesis. ### Competing Interest Statement The authors have declared no competing interest. Eunice Kennedy Shriver National Institute of Child Health and Human Development, https://ror.org/04byxyr05, NIHR01HD099816

doi.org

Colin Conine@colinconine.bsky.social · 3mo ago

And highlighted in Science! www.science.org/content/arti...

Sperm carry unexpected genetic messages

Maturing mouse sperm get loaded with full-length messenger RNAs that are transferred to fertilized egg, suggesting a new route for paternal influence

science.org

Nat Trigg@natrigg.bsky.social · 4mo ago

Now published in NAR - doi.org/10.1093/nar/... Huge thanks to @colinconine.bsky.social and co-authors @alexisgleach.bsky.social @gracelee.bsky.social Check out our accompanying web app to explore the data too. reprodatahub.shinyapps.io/Epididymal_m...