A microbial growth-coupled platform for in vivo interrogation of Rubisco oxygenase activity https://www.biorxiv.org/content/10.64898/2026.07.10.737725v1
Enrico Orsi
@eorsi.bsky.social
Bolognese 🇮🇹 in Copenhagen 🇩🇰 I engineer and optimize the metabolism of nonmodel C1-trophic bacteria for novel biotechnological applications Metabolic Engineering | Green Economy | CO2 valorization | DTU Biosustain
🧬 Even Earth's most abundant enzyme makes mistakes. Rubisco fixes CO₂ for nearly all life but wastes ~20% of potential crop yield on a 3-billion-year-old side reaction. We built a microbial sensor that finally makes it visible & tinkerable in living cells. www.biorxiv.org/content/10.6...
10 years ago today, Italian PhD student Giulio Regeni was found murdered after being kidnapped and tortured by Egyptian security services. Egypt has never cooperated with Italian prosecutors to identify those responsible www.channeldraw.org/2026/01/24/g...
Giulio Regeni. Until There Is Justice - ChannelDraw
An artistic journey at the heart of an open wound
channeldraw.org
Newest preprint from our lab: a new family of promising synthetic CO2 fixation cycles that may outcompete the Calvin cycle and that Vittorio Rainaldi realized to a great extent in E. coli! Up to 11 heterologous enzymes in a cascade supporting CO2 fixation & growth! www.biorxiv.org/content/10.1...
Modular in vivo engineering of the reductive methylaspartate cycles for synthetic CO2 fixation
Biological carbon fixation is currently limited to seven naturally occurring pathways. Synthetic carbon fixation pathways have the potential to surpass aerobic natural pathways in efficiency, but none...
biorxiv.org
Growth-coupled microbial biosynthesis of the animal pigment xanthommatin — Led by Leah Bushin, featuring M. Gracia Alvan, @danielvolke.bsky.social @oscarpuiggene.bsky.social in a fantastic collaboration w/Brad Moore @labnikel.bsky.social @natbiotech.nature.com 🦑 www.nature.com/articles/s41...
How does Pseudomonas putida keep its redox balance steady across different metabolic states? Our new preprint shows that two transhydrogenases -membrane-bound PntAB and soluble SthA - act together as a flexible, reversible system to control NAD(H)/NADP(H) ratios. www.biorxiv.org/content/10.1...
Integrated control of redox and energy metabolism by the membrane-bound and soluble transhydrogenases of Pseudomonas putida across metabolic regimes
Redox homeostasis is central to microbial physiology and stress adaptation, yet the functional roles of transhydrogenases remain poorly understood beyond a few organisms. In this study, we systematica...
biorxiv.org
🔥🔥🔥 Friday Evening, Paper Out, Feierabend 🔥🔥🔥 When evolution and enzyme engineering team up, they can convince microbes to assimilate sustainable carbon substrates preparing them for a life in a circular bioeconomy. doi.org/10.1016/j.ym...
Redirecting
doi.org
Please repost! Postdoc in adaptive laboratory evolution and C1 synthetic metabolism! Location: DTU Biosustain 🦠🧬🔬 Starting in 01/2026! Fell free to reach out if you have questions! efzu.fa.em2.oraclecloud.com/hcmUI/Candid...
efzu.fa.em2.oraclecloud.com
🧪 We're hiring a #Postdoc in Bioprocess Engineering! Excited about scaling CO₂-based biomanufacturing from bench to pilot scale? Join our group working on converting CO₂ + renewable electricity → polymers 💡🌱 👇
#ME16 This is quite subjective , but imo @helenasm.bsky.social just gave the most impressive talk of the conference so far and she is "only" in the third year of her PhD. If you are interested in synthetic pathway engineering or C1 fixation you should check out her research.
scholar.google.com
🎉 Excited to share that I’ve received an Emerging Investigator Grant from the Novo Nordisk Foundation! I’ll start my research group in Jan 2026 to develop universal cell factories for P2X & CO₂-based biomanufacturing 🌱🔬 👉 researchleaderprogramme.com/recipients/e...
www.sciencenews.dk/en/designed-... Piece from ScienceNews.dk on our glyoxylate and glycolate sensor strains
Designed bacteria can detect specific molecules
Researchers have developed a computer-guided method for designing and engineering bacteria that can detect the presence of specific molecules in the environment. This method will advance the use...
sciencenews.dk
Excited to share a main project from my PhD, out now in @naturecomms.bsky.social! 📝 We've designed and brought to life the “CORE cycle” – a new-to-nature pathway that provides a novel route for biological CO2 capture 🦠🌱 nature.com/articles/s41... Take a look! Thread below... 🧵
Interested in engineering bacteria to turn #C1feedstocks into high-value chemicals for #bioproduction? Here is a thread summarizing our latest review on the roadblocks and how we might bio-hack our way past them 🧵 1/n academic.oup.com/femsre/advan...
Seven Critical Challenges in Synthetic One-Carbon Assimilation and Their Potential Solutions
Abstract. Synthetic C1 assimilation holds the promise of facilitating carbon capture while mitigating greenhouse gas emissions, yet practical implementatio
academic.oup.com
Online now: Streamlined and efficient genome editing in Cupriavidus necator H16 using an optimised SIBR-Cas system
Streamlined and efficient genome editing in Cupriavidus necator H16 using an optimised SIBR-Cas system
This study developed two simple, efficient, and rapid genome editing tools termed Self-splicing Intron-Based Riboswitch-Cas9 (SIBR-Cas9) and SIBR2.0-Cas12a, for editing the genome of the industrially relevant microbial strain Cupriavidus necator H16.…
dlvr.it
Excited to see our SIBR work out in Trends in Biotechnology :) doi.org/10.1016/j.ti... This is the first study I have coordinated from start to finish, and it brings me immense satisfaction to see it published. For a brilliant description of the tech check this: www.linkedin.com/feed/update/...
Redirecting
doi.org
Our brand new CRISPR/Cas toolkit for genome editing of Cupriavidus necator is out🦠🧬 A joint effort w/ @dellavallesimo.bsky.social and Costas (and many more!) Check 🧵 our long journey for developing a new state-of-the-art for the manipulation of this promising bug 1/n www.cell.com/trends/biote...
Streamlined and efficient genome editing in Cupriavidus necator H16 using an optimised SIBR-Cas system
This study developed two simple, efficient, and rapid genome editing tools termed Self-splicing Intron-Based Riboswitch-Cas9 (SIBR-Cas9) and SIBR2.0-Cas12a, for editing the genome of the industrially ...
cell.com
📑📑📑#synthetic_biology @eorsi.bsky.social @pabnik.bsky.social present a workflow for designing versatile auxotrophic metabolic sensors for glyoxylate and glycolate detection @naturecomms.bsky.social www.nature.com/articles/s41...
Computation-aided designs enable developing auxotrophic metabolic sensors for wide-range glyoxylate and glycolate detection - Nature Communications
Auxotrophic metabolic sensors (AMS) are vital for bioengineering but are often time-consuming to develop. Here, the authors present a workflow for designing versatile AMS, demonstrating their use for ...
nature.com
The utilization of #C1-feedstocks, such as methanol, by non-model bacteria demands an in-depth analysis of design alternatives, a modular strategy to identify bottlenecks and efficient solutions within the host #metabolism of the chosen host... www.biorxiv.org/content/10.1...
Systematic engineering of synthetic serine cycles in Pseudomonas putida uncovers emergent topologies for methanol assimilation
The urgent need for a circular carbon economy has driven research into sustainable substrates, including one-carbon (C1) compounds. The non-pathogenic soil bacterium Pseudomonas putida is a promising ...
biorxiv.org
Check out Sebastians crazy effort to engineer E. coli to grow on formate via the synthetic Serine-Threonine-Cycle. In contrast to the rGlyp the STC works even at ambient CO2!🔥
Sebastian Wenk@sebwenk.bsky.social · last yr.Interested in Sustainability, Metabolic Engineering and Evolution? Check out how we convinced E. coli to grow on the CO2-derived substrate formic acid using the synthetic Serine Threonine Cycle! ♻️🌱🦠 OPEN ACCESS publication to be found here: 🚨 doi.org/10.1016/j.ym... 🚨 #MEvoSky #microsky (1/🧵)
Only one week left to apply for this exciting PhD position, supervised by @gillesvanwezel.bsky.social and me! Please apply if you are interested in microbial biochemistry and have a solid background in enzymes, metabolic pathways, and bacterial genetics. www.universiteitleiden.nl/en/vacancies...
PhD aminosugar metabolism
The Faculty of Science / the Institute for Biology is looking for a :PhD Candidate , Aminosugar metabolism and control of growth and development in bacteriaVacancy number: 15487 PhD project descriptio...
universiteitleiden.nl
Interested in Sustainability, Metabolic Engineering and Evolution? Check out how we convinced E. coli to grow on the CO2-derived substrate formic acid using the synthetic Serine Threonine Cycle! ♻️🌱🦠 OPEN ACCESS publication to be found here: 🚨 doi.org/10.1016/j.ym... 🚨 #MEvoSky #microsky (1/🧵)
🚀 The SEM Group (Lab Nikel) is officially on Bluesky! We’re kicking things off with our latest paper: an end-to-end journey on designing, implementing & applying auxotrophic metabolic sensors for bioengineering! 🧬🔬 @naturecomms.bsky.social Check it out: www.nature.com/articles/s41...
Computation-aided designs enable developing auxotrophic metabolic sensors for wide-range glyoxylate and glycolate detection - Nature Communications
Auxotrophic metabolic sensors (AMS) are vital for bioengineering but are often time-consuming to develop. Here, the authors present a workflow for designing versatile AMS, demonstrating their use for ...
nature.com
With great collaborations come great outcomes! A fine example from @eorsi.bsky.social from @pabnik.bsky.social's lab et collaborators of how to design, build and develop cell biosensors for value-added chemicals (related to synthetic C1 assimilation, of course)! 🧪
Enrico Orsi@eorsi.bsky.social · last yr.We just #published an end-to-end work for the computer-aided design💻, construction🛠️, validation✅, characterization🔬 & application💪 of #auxotroph metabolic sensors, the work-horses of in vivo growth-coupled selection screening systems-using glycolate as test case. 🧵👇1/ www.nature.com/articles/s41...
We just #published an end-to-end work for the computer-aided design💻, construction🛠️, validation✅, characterization🔬 & application💪 of #auxotroph metabolic sensors, the work-horses of in vivo growth-coupled selection screening systems-using glycolate as test case. 🧵👇1/ www.nature.com/articles/s41...
Really happy to share that our work on constructing six glyoxylate / glycolate sensors covering a three order of magnitude concentration range is out now in Nature Communications ! 🔥 Read below to see how we developed the sensors and what they are good for 🥳. www.nature.com/articles/s41...
Computation-aided designs enable developing auxotrophic metabolic sensors for wide-range glyoxylate and glycolate detection - Nature Communications
Auxotrophic metabolic sensors (AMS) are vital for bioengineering but are often time-consuming to develop. Here, the authors present a workflow for designing versatile AMS, demonstrating their use for ...
nature.com
Super excited to see my main PhD project finally out in @naturemicrobiol.bsky.social www.nature.com/articles/s41.... We show for the first time that a synthetic and engineered pathway can indeed exceed nature at one-carbon fixation.
One-carbon fixation via the synthetic reductive glycine pathway exceeds yield of the Calvin cycle - Nature Microbiology
An engineered one-carbon-fixation pathway increases biomass yields of Cupriavidus necator compared with the Calvin cycle and can support future, sustainable bio-based production.
nature.com
Preprint alert🚨Systematic #engineering of synthetic serine cycles in #Pseudomonas putida uncovers emergent topologies for #methanol assimilation · @biorxivpreprint.bsky.social led by Òscar Puiggené #SynBio #Methylotrophy #CircularEconomy www.biorxiv.org/content/10.1...
As 2024 wraps up🌇, I’ve been reflecting on the journey in #metabolic #engineering this year. I’m grateful for the opportunity to contribute to cool research, and thought I’d share a thread of what I’ve been involved with. Looking forward to connecting with the #biotech community here on Bluesky! 🙏🔬
