Join @pennmedicine.bsky.social on 6/15 at 2pm for their 4th Annual Juneteenth Seminar ft'ing keynote speaker @lydiagrmai.bsky.social (@yaleschoolofmed.bsky.social). Register➡️ tinyurl.com/3ubz3bkd

It was an honor to host @yiweichang.bsky.social from @upenn.edu @isbupenn.bsky.social for a seminar at @ohsunews.bsky.social's Department of Chemical Physiology and Biochemistry!

I am so happy to see our paper on spatial feature based analysis of chromatin states in print in Nature Communications. Here is an early access version of O-SNAP: www.nature.com/articles/s41...

Heterogeneity of late #endosome/#lysosomes shown by multiplexed DNA-PAINT imaging. Quantitative pipeline developed by Charles Bond, Melike Lakadamyali @melikel.bsky.social and colleagues: rupress.org/jcb/article/... 📕 Part of #Lysosomes 2026: rupress.org/jcb/collecti...

🚨first Bartman lab preprint! it's an LC-MS method to measure "total Coenzyme A" (sum of all acyl-CoAs). this will let us study coA synthesis flux using heavy-isotope tracing! huge congrats to first author Amelia Taylor and star coauthor @mzspectrum.bsky.social ! www.biorxiv.org/content/10.6...

Congratulations to @carlhjune.bsky.social (@pennpathlabmed.bsky.social), Katalin Kariko (Neurosurgery) & @drewweissmanlab.bsky.social (@pennmedicine.bsky.social) on being named to the @forbes.com list of top 250 living innovators! tinyurl.com/34mxafsk

We're super excited to share MissAlignment: a new ML-based approach to reference-free tilt series alignment, spearheaded by @martenchaillet.bsky.social. We think it's going to make your cryo-ET life a lot better. Preprint: www.biorxiv.org/content/10.6... Code: github.com/warpem/miss-... 🧶 Thread:

Preprint 🧵! I’m really excited to introduce BIGSMALL, an interleaved multi-magnification cryo-ET scheme bridging molecular and cellular scales in a single acquisition! BIGSMALL = 𝐁road 𝐈nformation 𝐆athering 𝐒trategy by 𝐌ultiscale 𝐀cquisition of lame𝐋𝐋a www.biorxiv.org/content/10.6... (1/6)

Mechanisms of disassembly at the actin filament pointed & barbed ends ft. @nickpalmer.bsky.social, Malgorzata Boczkowska, Grzegorz Rebowski & Roberto Dominguez (BBCB Graduate Group) @upennphysiology.bsky.social @science.org www.science.org/doi/10.1126/...

New research out in @natcomms.nature.com provides insight into the molecular mechanism of menthol-induced TRPV5 channel inhibition ft. José De Jesús-Pérez & Vera Moiseenkova-Bell (Systems Pharmacology & Translational Therapeutics) @isbupenn.bsky.social www.nature.com/articles/s41...

Congratulations to Nikolaos Sgourakis (@i3h-upenn.bsky.social), Mark Sellmyer (Radiology), @karanoelle.bsky.social (@penncancer.bsky.social) & @rdrapkin8-penn.bsky.social (OBGYN) on being named 2026 Early Detection Awardees! @themarkfdn.bsky.social @theaacr.bsky.social tinyurl.com/54pcj8dw

Cholesterol-containing lipid crystals can directly stiffen the rat steatotic liver before fibrosis ft. David Li, Rebecca Wells (@PennGIHep), Abigail Loneker, Jamie Ford (@pennengineering.bsky.social), Elaine Mihelc (@isbupenn.bsky.social) & Paul Janmey (Physiology) www.pnas.org/doi/10.1073/...

Congratulations to León Islas's and Vera Moiseenkova-Bell's lab members!!! Angélica Méndez-Reséndiz and José De Jesús-Pérez did amazing job on discovering how menthol inhibits TRPV5 channel. #cryoEM #TRPV #menthol #ColdSensation #ion #channels #calcium www.nature.com/articles/s41...

Join the Department of Biochemistry & Biophysics, the Johnson Foundation Biophysics & Structural Biology Core Facility, & the @isbupenn.bsky.social on 3/10 for their Symposium on Analytical Ultracentrifugation! Learn more & register➡️ tinyurl.com/47s2vnmf

Seeing mechanisms of disease Now online: “Seeing the mechanisms of disease with cryoEM” on Penn Medicine—how near-atomic views of molecules help us decode what goes wrong in illness. #cryoEM #SciComm www.pennmedicine.org/news/seeing-... @yiweichang.bsky.social @jrocereta.bsky.social

Freeze, image, cure Our cryoEM feature series is live on Penn Medicine: “Freeze, image, cure.” A closer look at how we visualize biology to understand disease and speed discovery. #cryoEM #ISB www.pennmedicine.org/news/freeze-...

Excited to share highlights of our recent discoveries by the Institute of Structural Biology at Penn Medicine. Made possible by our outstanding faculty, students, postdocs, and staff. www.pennmedicine.org/news/freeze-...

Wishing you & yours a restful & restorative holiday break! We'll see you back online in 2️⃣0️⃣2️⃣6️⃣! 💓✨

Visualization of lysosomal membrane proteins by cryo electron tomography ft. @bridget_mcveigh, José De Jesús-Pérez, @moiseenkovabell (@PennSysPharmATT), Prerana Gogoi, @MageswaranKumar & Elaine Mihelc (@isbupenn.bsky.social) @natcomms.nature.com www.nature.com/articles/s41...

Excited to share our latest @nature.com: How does naloxone (Narcan) stop an opioid overdose? We determined the first GDP-bound μ-opioid receptor–G protein structures and found naloxone traps a novel "latent” state, preventing GDP release and G protein activation.💊🧪 🧵👇 www.nature.com/articles/s41...

We’re proud to celebrate our institute associate director, Dr. Yi-Wei Chang, for being named the 2025 recipient of the Michael S. Brown New Investigator Research Award. @yiweichang.bsky.social

Congrats Bridget McVeigh, José De Jesús-Pérez, Elaine Mihelc & Vera Moiseenkova-Bell on your first cryoET publication "Visualization of lysosomal membrane proteins by cryo electron tomography" #cryoET #lysosomes #TRPML1 www.nature.com/articles/s41...

Visualization of lysosomal membrane proteins by cryo electron tomography - Nature Communications

Lysosomes are vital for cellular health and linked to many diseases. Here, authors use cryo-ET to image intact lysosomes and resolve lysosomal protein structures, providing a platform to advance the s...

nature.com

Happy to announce "Technical Innovations Using #SerialEM: A Community Meeting", a new format of hybrid meeting David Mastronarde and I are organizing in April 2026. For more info, a preliminary list of speakers, pre-registration etc. visit www.nexperion.net/semcm2026

...and big thanks to @jiwasa.bsky.social for making this amazing video to illustrate the strand-sliding mechanism!

Excited to share our new @science.org paper! Led by postdocs Ruchao Peng and Xin Xu, we used cryo-EM/ET to reveal the influenza ribonucleoprotein complex structure and its strand-sliding mechanism for RNA synthesis, paving the way for new antivirals. www.science.org/doi/10.1126/...

Molecular basis of influenza ribonucleoprotein complex assembly and processive RNA synthesis

Influenza viruses replicate and transcribe their genome in the context of a conserved ribonucleoprotein (RNP) complex. By integrating cryo–electron microscopy single-particle analysis and cryo–electro...

science.org

Congratulations to Drs. Lynch, Burslem, Marmorstein, Yi-Wei, and the entire research team on the publication of your latest research article in the newest issue of Science magazine! | Science www.science.org/doi/10.1126/...

Molecular basis of influenza ribonucleoprotein complex assembly and processive RNA synthesis

Influenza viruses replicate and transcribe their genome in the context of a conserved ribonucleoprotein (RNP) complex. By integrating cryo–electron microscopy single-particle analysis and cryo–electro...

science.org

Join the Institute of Structural Biology on 5/21 for the 4th Structural Biology Symposium! Learn more & register➡️ isb.med.upenn.edu/4th-structur... @BB_UPenn

🔬 The 4th Structural Biology Symposium is almost here! Don’t miss out—there’s still time to register. bit.ly/4iM5QAm

Excited to see this out in @science.org today!! www.science.org/doi/10.1126/...

Intracellular protein editing enables incorporation of noncanonical residues in endogenous proteins

The ability to study proteins in their native cellular context is crucial to our understanding of biology. In this work, we report a technology for intracellular protein editing, drawing from split in...

science.org

George Burslem@gburslem.bsky.social · 2y ago

Ever wanted to edit a protein sequence inside a living mammalian cell? Maybe to site specifically incorporate a non-canonical residue? Perhaps you’d like to do a pull down without an antibody? Or use a small molecule fluorophore to image an endogenous protein? 1/4