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Miranda Marvel

@mmarvel.bsky.social

Researching the epigenetic regulation of vascular development in #zebrafish and #cavefish in the #WeinsteinLab

10 Posts505 Follower561 Folgt
Leah Greenspan@ljgreenspan.bsky.social · 8mo ago

Excited to have started my new lab in Human Genetics at Pitt Public Health! We study wound healing in diabetes & aging using adult zebrafish. Check out this movie showing neutrophil dynamics after injury. Now recruiting research staff, grad students, and postdocs. DM me if interested.

Dan 🐟 C@zebrafish007.bsky.social · last yr.

@jimmykjm.bsky.social and @isabellaclsci.bsky.social et al. focus on epigenetic changes during fin regeneration in this preprint: www.biorxiv.org/content/10.1...

Early epigenetic priming of regeneration studied with a novel transgenic epigenetic reporter

Tissue regeneration requires previously differentiated cells to regain developmental plasticity. However, the upstream mechanisms initiating this process remain poorly understood. Here, we leverage a ...

biorxiv.org

Katherine W. Rogers@katwrog.bsky.social · last yr.

Happy to share a new preprint from my lab! We characterize the on/off kinetics, light dosage-dependence, and more for a suite of optogenetic signaling activators in zebrafish embryos 💡 www.biorxiv.org/content/10.1...

An optogenetic toolkit for robust activation of FGF, BMP, and Nodal signaling in zebrafish

Cell signaling regulates a wide range of biological processes including development, homeostasis, and disease. Accessible technologies to precisely manipulate signaling have important applications in ...

biorxiv.org

Siegenthaler Lab@siegenthalerlab.bsky.social · last yr.

Great new work led by @biomarina-vg.bsky.social on zebrafish meninges! Particularly 🤩 is the descriptions of ependymin-expressing meningeal cells (not a fibroblast!) & ablation studies showing their essential role in viability. So much more to learn! Congrats Marina & team on this great work! 🦓🐟🧠

Adult zebrafish possess complex multilayered cephalic meninges. Transmission electron micrograph (TEM) images of transverse sections through the dorsal heads of adult zebrafish, at the level of the optic tecta. A, Pseudocolored overview image of the cephalic meninges, revealing multiple tissue layers between the skull and the brain parenchyma. B, Dorsal surface of a dissected adult zebrafish brain, showing the leptomeninges partially peeling away from the underlying brain parenchyma. C, Higher magnification image of the boxed region in panel B, showing multiple leptomeningeal cell layers separated by membrane bilayers (red arrowheads), delimited ventrally by a well-defined basal lamina (pseudocolored purple). D, Nucleated red blood cell-containing blood vessel embedded within the leptomeninges, with desmosomes and junctions connecting individual endothelial cells (arrows). E, Higher magnification image of the boxed region in panel D, showing a portion of the vessel wall with a characteristic endothelial cell-cell junction (arrow). F, Higher magnification image of collagen fibers in the adult zebrafish leptomeninges. G, Pseudocolored overview image of the cephalic meninges, showing separated heavily pigment dura mater (dural meninges) and leptomeninges. multiple tissue layers (pseudocolored) between the skull and the brain parenchyma. H, Higher magnification image of the boxed region in panel G, showing bilayered dural meninges with periosteal dura adjacent to the skull with an underlying meningeal dural cell layer with numerous large melanophore pigment granules (arrows). Scale bars = 3 µm (A), 4 µm (B,G), 1 µm (C,H), 2 µm (D), 0.3 µm (E), and 0.2 µm (F).M, Dorsal view of an adult casper zebrafish head with the optic tectum and cerebellum clearly visible through the thin, translucent skull. The yellow box shows the approximate region depicted in panels N and O. N,O, Confocal images of the meninges imaged through the intact skull of a living Tg(mrc1a:egfp)y251, Tg(epd:mcherry)y715 double transgenic adult zebrafish, showing (N) mrc1a:egfp-positive FGPs and lymphatics (green) and epd:mcherry-positive LBCs (magenta), or (O) only epd:mcherry-positive LBCs (magenta). P, High-resolution, high magnification confocal Z-stack through the leptomeninges on the surface of a dissected Tg(mrc1a:egfp)y251, Tg(epd:mcherry)y715 double transgenic adult zebrafish brain, showing LBCs (magenta) and FGPs (green). The large center image is a dorsal XY view of the reconstructed image stack, and the images below and to the right show single-plane XZ and YZ views along the planes noted by the dashed lines (xz-1, xz-2, yz).
Zebrafish Rock! 🎃@zebrafishrock.bsky.social · last yr.

“These findings support the use of zebrafish as a useful comparative model for studying the meninges, provide a foundational description for future zebrafish meningeal research” New work from @biomarina-vg.bsky.social & Weinstein Lab at NIH 🐟

Point Defiance Zoo & Aquarium@ptdefiancezoo.bsky.social · 2y ago

Exciting news! We’re thrilled to announce that tapir Yuna gave birth to a rare and endangered Malayan tapir calf Sunday night. The newborn, covered in distinctive white spots and stripes resembling a fuzzy walking watermelon, is only the second tapir born in our 120-year history.

James Briscoe@jamesbriscoe.bsky.social · 2y ago

A couple of weeks left to apply to join Development's *Pathway to Independence* programme Support & mentoring for post-docs on the academic job market Deadline: January 31 journals.biologists.com/dev/pages/pi...

Development PI Programme | Development | The Company of Biologists

Development PI Programme | Development | The Company of Biologists Development's Pathway to Independence programme Development is excited to announce our second call for our Pathway to I...

journals.biologists.com

Natasha O'Brown@natashaobrown.bsky.social · 2y ago

And somehow I missed it going live last week 😂 - our #zebrafish glial-vascular paper is available for those finterested! One of the best ways to end 2024 as a #newPI www.cell.com/iscience/ful...

Zebrafish glial-vascular interactions progressively expand over the course of brain development

Glial-vascular interactions are critical for the formation and maintenance of brain blood vessels and the blood-brain barrier (BBB) in mammals, but their role in the zebrafish BBB remains unclear. Using three glial gene promoters—gfap, glast, and glastini (a truncated glast)—we explored glial-vascular development in zebrafish. Sparse labeling showed fewer glial-vascular interactions at early stages, with glial coverage and contact area increasing with age. Stable transgenic lines for glast and glastini revealed similar developmental increases, starting at ∼30% coverage at 3 days post-fertilization (dpf) and peaking at ∼60% by 10 dpf, and consistently higher glial coverage in the forebrain and midbrain than in the hindbrain.

cell.com

Ben Hogan@benjhogan.bsky.social · 2y ago

Latest preprint from the lab! Vascular mural cells protect the adult brain from haemorrhage but do not control the blood-brain barrier in developing zebrafish www.biorxiv.org/content/10.1...

Vascular mural cells protect the adult brain from haemorrhage but do not control the blood-brain barrier in developing zebrafish

The blood-brain barrier (BBB) protects the brain from circulating metabolites and plays central roles in neurological diseases. Endothelial cells (ECs) of the BBB are enwrapped by mural cells includin...

biorxiv.org

Laurent Formery@laurentformery.bsky.social · 2y ago

Shamelessly taking advantage of the bluesky migration to share again some of my favorite echinoderm pics 😀🤩 🔽 Acetylated tubulin (nerves and cilia) and phalloidin (muscles) stains in a juvenile sea urchin (Paracentrotus lividus) just after metamorphosis ⭐

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