Cell-to-cell cooperation through membrane vesicles enhances antibiotic persistence @science.org @bcmfromthelabs.bsky.social www.science.org/doi/10.1126/... www.science.org/doi/10.1126/...
See-Yeun Ting 陳詩允
@seeyeunting.bsky.social
PI in the Institute of Molecular Biology at Academia Sinica. Studying polymicrobial interaction in bacterial community. http://www.thetinglab.com
In a series of workshops last year, we discussed the term ‘synthetic community’ and its usage. It’s so loosely defined and is regularly used to define something which in my eyes isn’t a SynCom. The discussion was also touching on whether the term itself is appropriate.
Excited to share our new preprint on a question that has puzzled us for a long time: How can the bacterial nano-weapon, type VI secretion system (T6SS), load and deliver so many antibacterial toxins that are completely different in structure and function? www.biorxiv.org/content/10.6...
biorxiv.org
New online! Revisiting the life cycle of temperate phages
Revisiting the life cycle of temperate phages
Nature Reviews Microbiology, Published online: 20 May 2026; doi:10.1038/s41579-026-01318-7The life cycle of temperate bacteriophages involves lytic or lysogenic cycles and has historically served as a model for studying genetic regulation. This Review provides an updated overview of these cycles and highlights their complexities, supporting a greater appreciation of the ecological roles of temperate phages.
dlvr.it
Excited to learn that Cpe1-like cysteine protease toxin is also employed by bacteria for bacterial competition via CDI (contact dependent growth inhibition). Type II topoisomerases are the hot spot targets!
Redox regulated auto-processing controls delivery of an antibacterial cysteine peptidase toxin https://www.biorxiv.org/content/10.64898/2026.05.07.723447v1
Redox regulated auto-processing controls delivery of an antibacterial cysteine peptidase toxin https://www.biorxiv.org/content/10.64898/2026.05.07.723447v1
During microbial warfare, #bacteria deploy toxins to inhibit or kill competitors. @ebayersantos.bsky.social &co reveal lipid-targeting #antibacterial #toxins & novel toxin domains within #Salmonella T6SS effectors, broadening known toxin enzymatic diversity @plosbiology.org 🧪 plos.io/40zTyEP
Still not in the final format but couldn't wait to share it with you, after a long jpurney to get here. Our latest work (with @davidalbesa.bsky.social lab) on Tke5 T6SS toxin from my favourite bug P. putida KT2440. 🧵 1/5 www.nature.com/articles/s42...
Tke5 is a Pseudomonas putida toxin that kills plant pathogens by depolarising membranes - Communications Biology
Physiological and biophysical studies of Tke5 reveal is a pore-forming toxin that kills resilient phytopathogens via ion-selective membrane depolarisation. Neutralised by Tki5, Tke5 offers a targeted ...
nature.com
1/2 "All T6SS-inducing regulators are equal, but some regulators are more equal than others". Preprint 🚨: We use #Vibrio to show that #T6SS activation by regulator manipulation may result in the expression of different effector repertoires, affecting toxicity 🦠 www.biorxiv.org/content/10.6...
It is widely accepted that #T6SS -mediated intoxication occurs only on solid surfaces, where prolonged cell-cell interactions are forced, and not in liquid environments. But is this generalization true? Our new preprint says it isn't. A 🧵 ... biorxiv.org/content/10.6... 🦠
Aeromonas adhesins facilitate kin and non-kin attachment to enable T6SS-mediated antagonism in liquid
Bacterial ability to deploy the type VI secretion system (T6SS) against rivals requires prolonged cell-cell interactions. Such interactions are facilitated on solid surfaces but are assumed to be absent in liquid, leading to the conventional dismissal of T6SS-mediated competition in liquid environments. Here, we find that Aeromonas jandaei employs its T6SS to eliminate diverse bacterial competitors in liquid media. Using a workflow that monitors interbacterial competition via prey luminescence, we demonstrate that auto-aggregation and co-aggregation, facilitated by distinct adhesins, enable kin and non-kin recognition and intoxication in a T6SS-dependent manner. Furthermore, we show that another marine bacterium, Vibrio coralliilyticus , employs T6SS to intoxicate rivals in liquid media. Collectively, our results indicate that T6SS-mediated competition in liquid is more common in marine bacteria than previously anticipated, and can be facilitated by diverse molecular mechanisms that govern cell aggregation. ### Competing Interest Statement The authors have declared no competing interest. Israel Science Foundation, https://ror.org/04sazxf24, 1362/21, 2174/22 Swiss National Science Foundation, 51NF40_180541
biorxiv.org
New lab preprint! Andrew Frando, Bobby Parsek, and Graham Roberts describe how components of the quinolone quorum sensing circuit of P. aeruginosa interact in competition with another Gram-negative bacterium, S. maltophilia -- it's a three-component AND logic gate. www.biorxiv.org/content/10.6...
biorxiv.org
Congratulations to the new cohort of 28 EMBO Young Investigators! They are joining an international network of more than 800 life scientists – Welcome! Read the press release here: https://www.embo.org/press-releases/twenty-eight-group-leaders-become-embo-young-investigators/
Be our IMB colleague! Join us for cool science! [open faculty position at the institute of molecular biology, academia sinica in taiwan] www.nature.com/naturecareer...
OPEN FACULTY POSITION, INSTITUTE OF MOLECULAR BIOLOGY, ACADEMIA SINICA, TAIWAN - 128 Academia Road, Section 2, Nankang, Taipei, Taiwan job with Institute of Molecular Biology, Academia Sinica, Taiwan ...
One tenure-track faculty position is open for a qualified individual to establish an active research program at the IMB.
nature.com
Excited to learn that our story on characterizing an interbacterial protease toxin is finally out @plosbiology.org "An interbacterial cysteine protease toxin inhibits cell growth by targeting type II DNA topoisomerases GyrB and ParE". Led by my wonderful team at Academia Sinica!
Always love a good bacterial warfare study: "Horizontal gene transfer of molecular weapons can reshape bacterial competition" by @prokaryota.bsky.social, @jdpal.bsky.social, et al. in @plosbiology.org journals.plos.org/plosbiology/...
Horizontal gene transfer of molecular weapons can reshape bacterial competition
Bacteria use molecular weapons such as toxins to outcompete rivals, but their horizontal gene transfer can undermine this advantage. This study shows that horizontal gene transfer of toxin plasmids is...
journals.plos.org
OUT NOW: Inducible transposon mutagenesis identifies bacterial fitness determinants during infection in mice #microsky 🧪 www.nature.com/articles/s41...
Inducible transposon mutagenesis identifies bacterial fitness determinants during infection in mice - Nature Microbiology
InducTn-seq, a method for inducible mutagenesis followed by transposon insertion site sequencing, enables temporal control of transposition to bypass population bottlenecks and enable the quantificati...
nature.com
Our Phage infection/T6S story is finally out @embojournal.org! "Surface-mediated bacteriophage defense incurs fitness tradeoffs for interbacterial antagonism" Led by my wonderful team at Academia Sinica! www.embopress.org/doi/full/10....
A҉L҉T҉R҉U҉I҉S҉T҉I҉C҉ ҉Z҉O҉M҉B҉I҉E҉ ҉B҉A҉C҉T҉E҉R҉I҉A҉🦠🧟 Fascinating Nat Comm paper Martin Cann Lab Durham UK Dead bacteria encode post-mortem protein catabolism via Lon protease—provides nutrients promoting growth of surviving bacteria No benefit of Lon to live bacteria to account for this www.nature.com/articles/s41...
Check it out! Another novel RNA modification - ADP-ribosylation - that was previously only known on proteins. A cousin to #glycoRNA 👏 www.nature.com/articles/s41...
Discovery of a distinct BAM complex in the Bacteroidetes https://www.biorxiv.org/content/10.1101/2025.01.31.636011v1
A ubiquitin-like protein controls assembly of a bacterial Type VIIb secretion system https://www.biorxiv.org/content/10.1101/2025.01.24.634720v1
Beautiful #symbiosis paper. Symbiosis and horizontal gene transfer promote herbivory in the megadiverse leaf beetles www.cell.com/current-biol...
Symbiosis and horizontal gene transfer promote herbivory in the megadiverse leaf beetles
Leaf beetles are among the most speciose herbivores on earth. Kirsch et al. show that multiple independent symbiont acquisitions and microbe-to-host horizontal gene transfers shaped the beetles’ reper...
cell.com
What do bacterial cells do when they run out of nutrients? Although most bacterial studies focus on cells in exponentially growing states, in the wild bacteria likely spend most of their time slowly starving to death. 1/n www.biorxiv.org/content/10.1...
E. coli prepares for starvation by dramatically remodeling its proteome in the first hours after loss of nutrients
It is widely believed that due to nutrient limitations in natural environments, bacteria spend most of their life in non-growing states. However, very little is known about how bacteria change their p...
biorxiv.org
Breaking Barriers: Gender Equity in Science, Institute of Molecular Biology, Academia Sinica, Taiwan. Together, stronger! www.youtube.com/watch?v=c4GE...
Breaking Barriers: Gender Equity in Science at IMB Academia Sinica
YouTube video by Institute of Molecular Biology 中央研究院 分子生物研究所
youtube.com
OUT NOW - Streptomyces secretes a siderophore that sensitizes competitor bacteria to phage infection www.nature.com/articles/s41...
Streptomyces secretes a siderophore that sensitizes competitor bacteria to phage infection - Nature Microbiology
A secondary metabolite sensitizes competitor Bacillus subtilis to a wide panel of lytic phages by sequestering iron and preventing the activation of Spo0A.
nature.com
New year, new paper! Now published in @nature.com. We identified and characterised diverse immune cell states in plants under pathogen attack. My postdoc work in the Ecker lab at @salkinstitute.bsky.social. A thread (0/n) #PlantScience www.nature.com/articles/s41...
🚨New review🚨 Evolution and ecology of anti-defence systems in phages and plasmids Link: www.cell.com/current-biol...
Ten species comprise half of the bacteriology literature, leaving most species unstudied https://www.biorxiv.org/content/10.1101/2025.01.04.631297v1
Starvation of the bacteria Vibrio atlanticus promotes lightning group-attacks on the dinoflagellate Alexandrium pacificum https://www.biorxiv.org/content/10.1101/2024.12.18.629110v1
The bully phage: A Shiga toxin-encoding prophage interferes with the induction of co-hosted prophages https://www.biorxiv.org/content/10.1101/2025.01.01.630987v1