Sonja Welsch

@sonjawelsch.bsky.social

Taking care of Electron Microscopy @mpibp.bsky.social. Yay for #cryoEM and #cryoET ❄️🔬but frozen is not always better. Also, #viruses 🦠 rule.

What is the best way to distribute the electron dose across your tilt series in cryo ET? Thanks to Maarten Tuijtel, we finally know --> head over to eLife for the publication! doi.org/10.7554/eLif...

Optimising the tilt-increment for in situ cryo-electron tomography

doi.org

Max Planck Institute of Biophysics@mpibp.bsky.social · last mo.

1/3: We are excited to share a new study by Maarten Tuijtel from @becklab.bsky.social, published in eLife. 🔬 🎉 The work addresses a key question in cryo-electron tomography: how to best distribute the limited electron dose across a tilt series while balancing angular sampling and image quality. 💥

Cryo-EM images showing sample structures at tilt increments from 1 to 10 degrees, with resolution patterns and measured values in nanometers.

Better vitrification = better cryo-ET. We've opened an RFA for two-year grants to advance vitrification techniques for biological samples—welcoming applications from researchers in heat transfer, cryogenics, and materials science. Apply here: bit.ly/4uip6eJ

@mgflast.bsky.social showing us how to do cellular structural biology on easymode thanks to his collection of pretrained nets 😎 Particularly cool: he demonstrates custom tomo picking workflows e.g. yTuRC at the minus end of MTs, restricting picks to specific cellular compartments Check it out!

Allegretti Lab@matteoall.bsky.social · 2mo ago

We're happy to announce our new preprint! 🐸 easymode: general pretrained networks for cellular cryo-ET. Segment ~20 cellular features – ribosomes, microtubules, mitochondria, nuclei & more – with zero model training. 🔗 doi.org/10.64898/202... 🧵👇

If you’re looking for a dream combo postdoc position that includes #cryoET plus #virology plus a scientifically and geographically very appealing lab: please consider applying 👇🏻🤩

Lars-Anders Carlson@lacarlson.bsky.social · 3mo ago

Open #postdoc fellowship in my group. Virus replication and cryo-ET. Projects can be tailored to ambitious candidates with diverse backgrounds, so don't hold back from applying! DM for more info. Deadline 22May. Apply here: umustipendie.varbi.com/en/what:job/... #virus #virology #teamtomo #cryoEM

New preprint 🚀 ❄️ We developed Autogrid-compatible HPF planchettes that enable direct freezing of clipped EM grids and streamline integration with cryo-FIB workflows ✔️ improved grid stability ✔️ reduced handling ✔️ robust vitrification (🦠 🧫 🧠) A step toward routine HPF for complex samples #teamtomo