SPEx: Compartment-Resolved Proteomics via Expansion Microscopy–Guided Microdissection

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biorxiv.org

biorxiv.org

biorxiv.org

Ca2+ and DRP1 drive endocytic lysosome reformation at tripartite contact sites

Lysosomes are essential in maintaining cellular health. Endocytic lysosome reformation (ELR) regenerates functional lysosomes following degradation of endocytic cargo, yet the mechanisms driving this process remain largely unknown. Here, we define the molecular machinery underlying ELR. We find that unlike autophagic lysosome reformation (ALR), ELR proceeds independently of mTOR and dynamin 2, but requires the mitochondrial fission GTPase DRP1. DRP1 mediates scission of endolysosomal tubules at contact sites with the endoplasmic reticulum (ER) and mitochondria. Disruption of DRP1 function or ER endolysosome contact results in elongated tubules, indicating defective lysosome reformation. Moreover, mitochondrial activity is essential for tubule initiation, and Ca2+ transfer from endolysosomes to mitochondria is crucial for ELR onset. Our findings reveal a dual role for mitochondria in ELR: first in ELR initiation and second in DRP1-dependent tubule fission at ER-mitochondria-endolysosome tripartite contact sites, uncovering the previously unappreciated role of mitochondria in endolysosome remodeling and fission. ### Competing Interest Statement The authors have declared no competing interest. Swiss National Science Foundation, 320030-231859, 310030-197779 University of Basel, https://ror.org/02s6k3f65

biorxiv.org

Bacterial RNA promotes proteostasis through inter-tissue communication in C. elegans

Nature Communications - Kyriakakis et al. show that bacterial RNA can improve C. elegans healthspan and reduce toxic protein aggregation in muscles. This depends on inter-tissue communication and...

rdcu.be

A CRISPR/Cas9 screen reveals proteins at the endosome-Golgi interface that modulate cellular anti-sense oligonucleotide activity

Nature Communications - Antisense oligonucleotide (ASO) cellular activity requires endosomal escape. Here, the authors show that disrupting Golgi-endosome protein AP1M1 enhances ASO activity by...

rdcu.be

Anne Spang awarded distinction of AAAS Fellow

The AAAS has elected Prof. Anne Spang at the Biozentrum, University of Basel, as a Fellow. With this honor, the world’s largest scientific society recognizes her outstanding contributions to the field...

biozentrum.unibas.ch

Coordination between ESCRT function and Rab conversion during endosome maturation | The EMBO Journal

imageimageEarly endosome maturation requires Rab conversion and ESCRT function; however, how these processes are coordinated remains largely elusive. This study proposes that Rab conversion in both C....

embopress.org