New paper! We tested #eDNA primers for detecting #fishes in Hawai‘i. 12S (MiFish, Riaz) performed best w/r/t fish sequence proportion. However, our major conclusion is that mock communities can lead you astray, since they don't accurately predict how primers will perform in a natural environment.
Metabarcoding Primers for Indo‐Pacific Fishes
We compared the relative performance of five metabarcoding primers (Berry 16S, Riaz 12S, MiFish E 12S, MiFish U 12S, and Leray CO1) to recover Indo-Pacific fish taxa. We tested the primers using temp...
dx.doi.org