Accidentally made this abomination in ImageJ this evening. I ran a max projection instead of a sum projection on a bunch of heat maps. It's not useful... but I can't stop staring at it.

This afternoon's imaging lab at #IALSJanelia is all about subcellular structures, highlighted here by myosin (white) and actin (orange). These membrane ruffles were captured on our lattice light-sheet microscope by @cmhobson.bsky.social

Excited to see this collaboration now in print. Dye chemistry meets AI-designed proteins. Three small, orthogonal tags; lifetime modulation; split versions. Request the free dyes at dyes.janelia.org www.science.org/doi/10.1126/...

De novo design of orthogonal far-red, orange, and green fluorophore-binding proteins for multiplexed imaging

Fluorescent proteins and small-molecule dyes offer complementary advantages for biological imaging: proteins are amenable to genetic tagging, whereas dyes provide superior brightness and photostabilit...

science.org

Ever seen mitochondria fly around like little rockets? 🚀 When cells divide, they don't just split DNA. I made a YouTube story based on a major Nature paper, showing how cells use actin waves to shuffle their powerhouses! 🔬 Watch the video here: youtu.be/udrVh_p7kCg #FluorescenceFriday #Microscopy

This mesmerizing timelapse captures the nonstop motion inside an animal cell. Red/orange streaks are the growing ends of microtubules, tiny “highways” that can rapidly assemble and disassemble to move proteins & other molecules within the cell. 📸: Andy Moore, HHMI’s Janelia Research Campus

Captured this clip when I was still working on the protein CYRI-A for my PhD and it remains one of my most favorites. It was my first time seeing lysosomes (🔴) fusing on and acidifying a macropinosome (⚪). I learned about this at uni but seeing it in action is something else! #FluorescenceFriday

#microtubules in orange hot, #F-actin greyscale, Fibroblast, spinning disc. @marcoenriquez18.bsky.social @aibnatuq.bsky.social #fluorescenceFriday

Check out our new release on BioRxiv co-lead by @pm-mueller.bsky.social 👉 rb.gy/lnrizk special🙏 to Severine Kunz #MDC @leventallab.bsky.social @andimicroscopy.bsky.social @ewerslab.bsky.social and Kedar Narayan @NCI CCR VolumeEM Are the days of discovering new cellular structures over?? /1

Out in @natmethods.nature.com: More dyes. They work. Quite well. And blink. Pick the one that fits your target, your technique, and your labeling density. With too many collaborators and institutes to list, but anchored at @hhmijanelia.bsky.social www.nature.com/articles/s41...

A series of spontaneously blinking dyes for super-resolution microscopy - Nature Methods

A series of spontaneously blinking dyes in the far-red range facilitate single-molecule localization microscopy. These dyes vary in their blinking properties and can be matched to the applications and...

nature.com

I am excited to share our most recent work collaborating with @centriolelab.bsky.social and @stearnslab.bsky.social to look at the ciliary base of mammalian multiciliated cells w/ cryo-ET, XL/MS, and U-ExM www.biorxiv.org/content/10.6...

Our paper is now out in Science! Super excited to share our discovery that #mitochondria #pearling is the elusive mechanism driving the regular distribution and inheritance of #mtDNA nucleoids 🧬 [1/6]

Not something you see in textbooks very often: tripolar mitosis.

An intracellular meteor shower. EB3 comets tracking growing microtubule plus-ends in a cultured cell.

Dynamic actin waves in a zebrafish embryo. Credit to @aaandmoore.bsky.social & Dvir Gur. #ZebrafishZunday 🧪

🧪 We are excited to share this novel open access paper on Phasor Mixing Coefficient to analyze colocalization, developed by folks @i2janelia.bsky.social and @aicjanelia.bsky.social. This is also the first technical paper jointly published with our sister imaging center @malacridalab.bsky.social!

Created using fluorescence microscopy, this stunning image of an animal cell showcases thin, web-like fibers called vimentin — a protein that provides mechanical stability to cells and tissues, despite a single vimentin being ~7000x smaller than the width of a human hair. 📸 @aaandmoore.bsky.social

🚨The Neurocyto lab is branching out in our latest preprint! We used tubulin microinjection to directly visualize microtubule turnover in developing hippocampal neurons, demonstrating the presence of in-lattice repair and a selective stabilization in the nascent axon. Check below, or read on 🧵 1/9

Direct labeling of microtubule turnover reveals in-lattice repair and stabilization patterns in developing neurons

The microtubule cytoskeleton is the backbone of neuronal morphogenesis, driving the development of the dendrites and axon, and supporting trafficking to distant compartments. How neuronal microtubules...

biorxiv.org

I don't always get pretty, isolated, AND transfected primary neurons in culture, but when I do I take advantage.... Rat hippocampal neuron overexpressing ThymosinB4-mScarlet and imaged for 16hr on a @zeiss-microscopy.bsky.social LSM880 with Airyscan. #FluorescenceFriday #Microscopy

This movie shows lysosomes (orange) and keratin (gray) in a cultured cell over 10 minutes.

I like this movie, but a friend of mine likes to complain about the obvious stitching artifacts. I'll try harder next time, Michael. Vimentin (orange) and ER (blue) in an overnight acquisition.

What's up Bluesky....here's some live-cell imaging taken in my lab

is there a direct crosstalk between actin and vimentin intermediate filaments? Our work shows that vimentin promotes actin assembly by stabilizing ATP-subunits at the barbed end. Fantastic work done by @lilianpaty.bsky.social with @romet-jegou-lab.bsky.social www.biorxiv.org/content/10.1...

Still posting cytoskeleton videos, it seems. Actin this time. Sample: Lifeact-eGFP in HeLa cells. Modality: Airyscan confocal Timestamp is mm:ss and the scale bar is 5 µm.

vimeo.com/1139537563?s... I uploaded a version of that vimentin IF movie to my vimeo page. It's still compressed, but looks a lot better. Best results when you set the quality to 4k.

24FPS_VimentinCos7GIF_4320p_ultraHQ

This is "24FPS_VimentinCos7GIF_4320p_ultraHQ" by Andy Moore on Vimeo, the home for high quality videos and the people who love them.

vimeo.com

Attempt number seven at uploading this video of intermediate filaments in an enormous COS7 cell. I have a feeling the BlueSky compression will not do it any favors.

iPS cell-derived cardiac myocytes (heart muscle cells) typically beat about once per second, so I usually speed up the movies I post; otherwise, scrollers might miss the action. But every now and then, a cell looks like this in real time. #CellBiology

www.nature.com/articles/s41... My dad passed away in September. In addition to being a brilliant scientist, he was an accomplished fisherman, a scholar of 17th-century English silver, and my hero. I miss him terribly.

In memoriam: Malcom A.S. Moore (1944–2025) - Leukemia

Leukemia - In memoriam: Malcom A.S. Moore (1944–2025)

nature.com

A fantastic paper by my @hhmijanelia.bsky.social colleague and friend Heejun Choi (who is on the job market!). Another elegant use of the Janelia Fluor dyes for cellular imaging. www.nature.com/articles/s41...

Secretome translation shaped by lysosomes and lunapark-marked ER junctions - Nature

Live-cell imaging of mRNA encoding secretome proteins and translated nascent peptide markers show that secretome translation occurs at endoplasmic reticulum junctions near lysosomes, requires lun...

nature.com

📕 Our Intermediate Filaments collection explores recent advances in intermediate filament research, focusing on lamins, keratins and vimentin. Explore new and recent JCB studies to accompany this week's European Intermediate Filament Meeting 👉 rupress.org/jcb/collecti... #Euro-IF

Very excited to share new work out today in @natchembio.nature.com on a new approach - FACES - for selectively imaging of phospholipids and other biomolecules at spatial resolutions down to individual membrane leaflets (1/n) www.nature.com/articles/s41...

Leaflet-specific phospholipid imaging using genetically encoded proximity sensors - Nature Chemical Biology

An approach combining bioorthogonal chemistry with genetically encoded fluorogen-activating proteins enables subcellular imaging of phospholipids and glycans, as well as the visualization of lipid tra...

nature.com