Being on summer holidays for so long that I forgot my work passwords ❤️
Aurélien Barbotin
@abarbotin.bsky.social
Engineer in optics and image processing
New paper alert 💥 "Spectral biophysical cytometry with nanosensors reveals remodelling of immune cells in atherosclerosis" Open access paper here: www.nature.com/articles/s41...
Spectral biophysical cytometry with nanosensors reveals remodelling of immune cells in atherosclerosis - Nature Nanotechnology
Membrane-permeant, environment-sensitive nanosensors enable high-throughput, multiparametric quantification of membrane order and mitochondrial potential, revealing immune-cell-specific remodelling an...
nature.com
And two more pre-prints from the lab, focusing on MreB polymerization cycle and dynamics! www.biorxiv.org/content/10.6... www.biorxiv.org/content/10.6...
A genetic screen unveils the polymerization cycle of the bacterial actin-like MreB
MreB, a bacterial actin homologue that organizes cell wall synthesis in most rod-shaped bacteria, assembles into membrane-associated filaments through mechanisms that have remained elusive despite dec...
biorxiv.org
Intrafilament nucleotide exchange in a prokaryotic actin homolog https://www.biorxiv.org/content/10.64898/2026.07.24.740542v1
An interesting observation we made in the lab before I left : membrane fluidity in B. Subtilis (and other Gram+) has two operating regimes in response to a change in temperature: constant between 28°C, linearly increasing above. We thought it would be constant! doi.org/10.64898/202...
it's the 10 year anniversary of a very important study
![In 2015, researchers Limb, Limb, Limb and Limb found that British doctors have surnames related to their specialties more often than expected by chance. Reproductive medicine had the largest proportion, with 1 in 52 doctors having specialty-relevant surnames including Horn, Hussey, and Woodcock. Second was Urology, with 1 in 59 related names such as Burns, Waterfall, Ball, and Koch.[96] "Dr Pain" appeared most commonly in general surgery.[96]](https://cdn.bsky.app/img/feed_thumbnail/plain/did:plc:cyyd366g3hrxp5rynixm2mm3/bafkreiddq3sctguxn4xgf4mfxyu43qh7e2htev3iktz5siwagfpdswfy3q)
If your test for ground-breaking discoveries can’t detect the discovery of RNAi, or of CRISPR-Cas9, or the cryoEM resolution revolution or Alphafold2, maybe it’s not a very good test. www.nature.com/articles/d41...
Retweeting this because I thought it was staph at first, and yet it isn't
France-BioImaging@france-bioimaging.bsky.social · last yr.Happy Saint Patrick!🍀 Meet Saccharomyces cerevisiae—the yeast behind beer!🦠It ferments sugars into alcohol & CO₂ in an oxygen-free environment. At Bordeaux Imaging Center, Isabelle Svahn captured it up close using scanning electron microscopy!🔬 👉france-bioimaging.org/node/bordeaux/
Happy Saint Patrick!🍀 Meet Saccharomyces cerevisiae—the yeast behind beer!🦠It ferments sugars into alcohol & CO₂ in an oxygen-free environment. At Bordeaux Imaging Center, Isabelle Svahn captured it up close using scanning electron microscopy!🔬 👉france-bioimaging.org/node/bordeaux/
"Teaching of genetics tends to begin with Mendelian traits, presumably because they are deemed easier to understand. This introduces misperceptions about genetic determinism, encouraging linear perspectives on genotype-phenotype relations." A call to embrace complexity by @philipcball.bsky.social.🧪
Should biology put complexity first?
The dictum “Everything should be made as simple as possible, but no simpler” poses a problem for biology. How simply can it be told without doing damage to its complex nature? The answer might be foun...
cell.com
New paper out 🎇 It has been 13 years since @owenlabuob.bsky.social and co. published a stellar protocol on how to measure membrane order with smart probes! Since then, there has been new dyes, imaging modes and biology! We wrote a protocol for Nature Protocols: www.nature.com/articles/s41...
Measuring plasma membrane fluidity using confocal microscopy - Nature Protocols
Membrane fluidity is a crucial feature in understanding cellular physiology. This protocol describes a robust pipeline for measuring plasma membrane fluidity using confocal imaging and new environment...
nature.com
The Indigenous People of North America have decided to go back to calling the Gulf of Mexico, “Chalchiuhtlicueyecatl”. It is what the Nahuatl People originally called it as it is the domain of the goddess Chalchiuhtlicue, who is assoc w/water bodies, including the Gulf of Mexico.
#BPS2025 is approaching! ✨🔬🧪 I will be presenting a poster on some TPE metabolic imaging and on a little bit of Phasor FLIM analysis. All recent and unpublished data. Sun Feb 16th - B548 Find me if you are there and interested and want to chat about NADH, FPs, FLIM, or FCS (always up for that).
#Cellpose 3 paper now out. Not all images are perfect. Restore your images with Cellpose3 to get better segmentations, w/ @marius10p.bsky.social www.nature.com/articles/s41...
After a long journey, Segment Anything for Microscopy is now published in Nature Methods! We significantly improve SAM for interactive and automatic segmentation in light and electron microscopy and build a user-friendly tool. www.nature.com/articles/s41...
I don't want a video explaining how to do the thing. I want step-by-step instructions in a plain text document, on a single page, ideally that I can see all of without scrolling while using a reasonably big font.
This looks like a terrific opportunity for anyone who wants to push the best microscopes to their limits!
Falk Schneider@faldalf.bsky.social · 2y ago🚨🚨Deadline approaching - last day to apply 🚨 Come and join a team of interdisciplinary microscopists as a postdoc at the University of Warwick All info below⬇️⬇️⬇️
'Barnett Rosenberg wasnt trying to cure cancer. He wasnt working on cancer. He wasnt working on any disease-related problem. He wasnt even working with human cells. All he was trying to do was to test a hypothesis about what would happen when dividing bacteria were placed in a strong electric field'
A Christmas Carol - Genome Biology
genomebiology.biomedcentral.com
Trying to find an old classic paper that was discussing how in science changes of scale induce a change of the rules, e.g explaining that chemistry is not applied physics, biology is not applied chemistry etc. Does it ring a bell with anyone ?
A question for membrane folks: should I expect a change of 3-5% percent in unsaturated fatty acids affect membrane viscosity? It is typically what people measure in cold shocks in bacteria but I find strange that such a small change would have an important effect
I'm a big fan of super plots, advocating it to everyone in the lab !
Sam Lord@samjlord.bsky.social · 2y agoQ: What's wrong with these plots? A: Pooling cell-level measurements across multiple runs conceals experimental reproducibility. Read more in JCB (with @fritzlaylin.bsky.social @katrinavelle.bsky.social & Dyche Mullins): rupress.org/jcb/article/...
Any more FFS/FCS Folks on bluesky? Where are you? Time to correlate. Please let me know who is on here and needs to be added. go.bsky.app/4BbiZyW
Allez le lire, c'est long mais très bien, notamment sur la puissance des images dans leur polysémie assumée et qu'on ne cherche pas à encadrer, sur la puissance évocatrice de la ville, et sur le "sens" de l'histoire (vers l'avant, c'est tout ce qu'il en dit). legrandcontinent.eu/fr/2024/07/3...
«Oui, ça ira». Une conversation fleuve avec Patrick Boucheron, co-auteur de la cérémonie d’ouverture des JO de Paris 2024 | Le Grand Continent
« On nous dit que nous sommes hors sol — mais le sol, il est là. » Succès planétaire, au cœur des polémiques, la cérémonie d’ouverture de Paris 2024 a fait date. Dans une prise de parole unique, extr...
legrandcontinent.eu