Robert E. Campbell
@campbell-lab.bsky.social
Professor in the Department of Chemistry, School of Science, The University of Tokyo. Editor-in-Chief of Protein Engineering, Design & Selection (PEDS). Opinions are my own. https://campbell.chem.s.u-tokyo.ac.jp
The hardest puzzle ever? It's got to be a protein! My hobby is turning protein structures into #OddlySatisfying 3D puzzles. Here's GFP (PDB: 1EMA)! 🧩🧬🧪 🎥 youtube.com/shorts/3ljlR... 📸 www.instagram.com/reel/Da2NKpT...
The hardest puzzle ever? It’s a protein!
YouTube video by Proteotopical
youtube.com
PinkyCaMP is finally published!! My first paper co-first authored with @sikmys.bsky.social where we make a great optogenetic and multiplex compatible red calcium indicator. Thank you to @massecklab.bsky.social and @campbell-lab.bsky.social for guiding this journey www.nature.com/articles/s41...
PinkyCaMP: an mScarlet-based calcium sensor with enhanced brightness, photostability and multiplexing capabilities - Nature Methods
PinkyCaMP is a red genetically encoded calcium indicator with improved brightness and photostability. Derived from mScarlet, it does not exhibit photoswitching upon blue light illumination and is ther...
nature.com
Finally online @natmethods.nature.com www.nature.com/articles/s41... This was a fun collaboration with @finkryan.bsky.social @massecklab.bsky.social @tpatriarchi.bsky.social @martinfuhrmann.bsky.social @campbell-lab.bsky.social @jangrundemann.bsky.social 🧪🧠🔬🧬
PinkyCaMP: an mScarlet-based calcium sensor with enhanced brightness, photostability and multiplexing capabilities - Nature Methods
PinkyCaMP is a red genetically encoded calcium indicator with improved brightness and photostability. Derived from mScarlet, it does not exhibit photoswitching upon blue light illumination and is ther...
nature.com
Jonas Wietek@jwietek.bsky.social · 8mo agoNew biosensor alert! 🧪 mStayGold(J)-based calcium ion and pH biosensors are now on bioRxiv. Excited to see how they fare in real applications! Reach out if you'd like to give them a whirl. www.biorxiv.org/content/10.6... www.biorxiv.org/content/10.6...
biorxiv.org
A Cinderella story: raising CLIP-tag from the ashes to shine in fluorescence labeling. www.biorxiv.org/content/10.6...
biorxiv.org
Marc Zimmer (gfp.conncoll.edu) makes a provocative case for establishing an official set of model proteins, with GFP as the prime example. Pro: GFP is uniquely AWESOME. Con: GFP is awesomely UNIQUE. In my view, the pros far outweigh the cons! academic.oup.com/peds/article... (paywalled, sorry)🧪
Green Fluorescent Protein
gfp.conncoll.edu
🧪 The Campbell lab at UTokyo is looking to hire a postdoctoral researcher for a project focused on protein engineering for optogenetic tool development. Details here: www.s.u-tokyo.ac.jp/en/recruit/?...
Open Positions - SCHOOL OF SCIENCE THE UNIVERSITY OF TOKYO
This is the open recruitment information for School of Science, the University of Tokyo.
s.u-tokyo.ac.jp
Thanks! Preprint is here: doi.org/10.1101/2025...
High-performance genetically-encoded green and red fluorescent biosensors for pyruvate
Pyruvate is the end-product of glycolysis and a central metabolite involved in many biochemical pathways. However, a lack of high-performance (i.e., Δ F / F > 10) single fluorescent protein (FP)-base...
doi.org
Addgene@addgene.bsky.social · 11mo agoGreenPy1 (GFP) & ApplePy1 (RFP): new pyruvate biosensors from @campbell-lab.bsky.social for multicolor imaging of metabolism. https://twp.ai/4iqyg5
Huge congratulations to Saskia on her first first-author paper! 🥳 chemistry-europe.onlinelibrary.wiley.com/doi/full/10....
Programming Green Light‐Responsive Hydrogels: From Photo‐Weakening to Photo‐Unresponsiveness and Photo‐Strengthening
The light response of CarHc protein-based hydrogels can be alterted from photo-weakening, to photo-strengthening, or becoming light-unresponsive depending on the added cofactors AdoCbl and Co2+. Unde....
chemistry-europe.onlinelibrary.wiley.com
Protein Engineering, Design & Selection (PEDS) invites contributions to a Special Collection titled, “Non-Canonical Amino Acids", with guest editors Prof. Huiwang Ai (Virginia) and Prof. Peng Chen (Peking). Send us your best work! academic.oup.com/peds/pages/c...
New tools! GreenPy and ApplePy are fluorescent biosensors for pyruvate with huge responses (20–40x in vitro) and a range of affinities (10s of μM to mM). These should be game changers for imaging of metabolism! Great work by Shosei Imai @sikmys.bsky.social and team 👏. www.biorxiv.org/content/10.1...
High-performance genetically-encoded green and red fluorescent biosensors for pyruvate
Pyruvate is the end-product of glycolysis and a central metabolite involved in many biochemical pathways. However, a lack of high-performance (i.e., Δ F / F > 10) single fluorescent protein (FP)-base...
biorxiv.org
I am delighted to share that I have taken on the role of Editor-in-Chief of Protein Engineering, Design & Selection. From the dawn of molecular biology to the current golden age of protein engineering, PEDS has been a cornerstone of the community. Send us your best work! academic.oup.com/peds
Protein Engineering, Design and Selection | Oxford Academic
Publishes research papers and review articles relevant to the engineering, design and selection of proteins for use in biotechnology and therapy, and for understanding fundamental properties of activi...
academic.oup.com
🚨iGluSnFR4 is finally out!🚨🧪 We present iGluSnFR4f and 4s, a novel pair of genetically-encoded glutamate indicators designed for high-fidelity imaging of synaptic activity in the living brain. ⬇️ www.biorxiv.org/content/10.1... 🎥 Below: iGluSnFR4s detecting minis in cultures w/ TTX #Neuroscience
Today we report the directed evolution of HUH into rHUH, a sequence-specific covalent protein tag for RNA labeling. rHUH was hard to engineer: the initial HUH template had no RNA activity, so we started with RNA-DNA hybrids. 30 rounds of selection on the yeast surface were required, and 7 cycles of
I, along with Dr. Sarah Shaykevich, will be speaking about red and near-infrared genetically encoded Ca2+ biosensors in the European Calcium Society (ECS) webinar on Wednesday, February 26th at 5 PM CET. This webinar series is open to everyone! docs.google.com/spreadsheets...
Doctoral student Dazhou Cheng's great work on developing a chemigenetic K+ indicator (doi.org/10.1021/jacs...) was featured in the Yomiuri Shimbun daily newspaper!
PhD students and postdoctoral researchers in the UK & Ireland can apply for a JSPS short-term (1-12 month) fellowship to study in Japan. Next deadline is Jan. 20 2025. If you are interested in engineering fluorescent biosensors, feel free to reach out! www.jsps.org/latest_calls...
JSPS London : Latest Calls FY2025 JSPS London Call for the Short Term Pre/Postdoctoral Fellowships for Research in Japan (First Call of Two for FY2025)
The Japan Society for the Promotion of Science (JSPS) is Japan's leading funding agency and is largely funded through annual subsidies from the Japanese Ministry of Education, Culture, Sports, Science...
jsps.org
Congratulations to the whole team! PinkyCaMP is a giant leap forward for red GECIs. Delighted to have contributed to the protein engineering with graduate student Shosei Imai.
Masseck Lab@massecklab.bsky.social · 2y ago🎄PinkyCaMP- our christmas gift to you! 🎁: We are excited to present PinkyCaMP, the first mScarlet based calcium sensor with: ✨exceptional, brightness ✨phototability ✨and optogenetic compatability. Check out our new preprint: www.biorxiv.org/content/10.1...
Ph.D. student Dazhou Cheng developed a chemigenetic K+ indicator (HaloKBP), using an rHCaMP/HaloCaMP-type design! pubs.acs.org/doi/10.1021/...
High-Performance Chemigenetic Potassium Ion Indicator
Potassium ion (K+) is the most abundant metal ion in cells and plays an indispensable role in practically all biological systems. Although there have been reports of both synthetic and genetically encoded fluorescent K+ indicators, there remains a need for an indicator that is genetically targetable, has high specificity for K+ versus Na+, and has a high fluorescent response in the red to far-red wavelength range. Here, we introduce a series of chemigenetic K+ indicators, designated as the HaloKbp1 series, based on the bacterial K+-binding protein (Kbp) inserted into HaloTag7 self-labeled with environmentally sensitive rhodamine derivatives. This series of high-performance indicators features high brightness in the red to far-red region, large intensiometric fluorescence changes, and a range of Kd values. We demonstrate that they are suitable for the detection of physiologically relevant K+ concentration changes such as those that result from the Ca2+-dependent activation of the BK potassium channel.
pubs.acs.org
The Asian Chemical Biology Initiative (ACBI) will hold tutorials and student interviews in Hanoi on January 19, 2025. Hope to see you there! Interested students can get more information and sign up here: www.asianchembio.com/hanoi-2025-interview Application deadline is December 1, 2024.
ACBI 2025 Hanoi Tutorial & Interview | ACBI
asianchembio.com
Excited to report an improved green fluorescent extracellular L-lactate biosensor (eLACCO2.1), and a new red fluorescent intracellular L-lactate biosensor (R-iLACCO1), for multiplexed fluorescence imaging! Deep appreciation for a great team of collaborators! rdcu.be/dpB8Y