Michaud, @andrewgoryachev.bsky.social, von Dassow, @xenresearchcenter.bsky.social et al. identify a versatile cortical pattern-forming circuit based on #Rho, F-#actin, Ect2, & RGA-3/4 rupress.org/jcb/article/... 📕 In "Collective Behaviors and Self-Organization in Cells" rupress.org/jcb/collecti...

#FENS2026 – Friday 11:30–13:00. Paul Lamothe-Molina @neuronblaze presents nLightG2/nLightR2, next-gen norepinephrine sensors for in vivo use. Come by to talk GPCR indicators, photometry, 2P imaging, multiplexing, or troubleshooting. Poster PS07-10AM-018. Paper: doi.org/10.1038/s415... @PatriarchiLab

Next-generation multicolor indicators for in vivo imaging of norepinephrine - Nature Methods

Improved red and green indicators for norepinephrine and their characterization are reported. These indicators allow detection of norepinephrine release in awake behaving mice in dual-color fiber phot...

doi.org

Gather round for the story of the Very Hungry Data Centre... 🧵 (1/4) #datacentre #ai

Photoswitchable optoGPCRs for reversible control of Gs and arrestin signalling www.biorxiv.org/content/10.64898/2026.07.01.735182v1

New paper! 🚀 A high-affinity anti- #mNeonGreen #nanobody turns #mNG into a #multifunctional Tag. @nanotag.bsky.social decided to openly share the #nanobody for research use. Not common, but we strongly believe well-defined reagents are key for reproducible science. We hope you find it useful! 💚

Difficult! We can use the Hill equation parameter estimates (GC8s: KD=46 nM, n=2.2; GC6f: 375 nM, 2.3) to compute the fraction of calcium-bound fluorophors as ~1% for GC6f and 55% for GC8s at 50 nM (~neuronal resting). Somehow the bound fraction for GC8s seems too high, though, indicating that ...

🟧 Orange is the new green?? 🟧 New sensitive orange calcium indicator (🟠OCaMP) now out in Nature Comms: www.nature.com/articles/s41... Highlights ⚡ Detects single action potentials in vivo 🔬 Optimized for 1030 nm imaging 📸 Highly photostable, minimal photoswitching 🌈 Multiplex with green biosensors

Looking through an old hard-drive and found a file called: "oil_immersion_lens.mp4" - a previously unseen #microscopistsNightmares?

New paper out: PinkyCaMP: an mScarlet-based calcium sensor with enhanced brightness, photostability and multiplexing capabilities.: Fink, Ryan; Imai, Shosei; Gockel, Nala Eileen; et al. Nature methods Vol. 23, no. 5, p. 998 - 1010 | Thanks for sharing #dzne #papers

Happy to reveal our latest work, Microbe-Independent Deep Assembly and Screening (MIDAS), a method for engineering proteins >10x more cheaply and quickly than before. MIDAS addresses the slowest step in protein engineering — construction and expression of variants — by skipping cloning entirely

Sarafan ChEM-H@stanford-chemh.bsky.social · 3mo ago

Faculty Fellow Michael Lin introduces MIDAS, a new method that builds and tests thousands of protein variants in days instead of weeks - delivering an order-of-magnitude improvement in speed and cost and advancing how proteins are engineered and studied:

Combining biosensor imaging data with an animated plot of the quantified intensity, based on a blog I wrote earlier: thenode.biologists.com/crafting-plo... In this version I used a dark theme

PinkyCaMP: a red genetically encoded calcium indicator with improved brightness and stability. @massecklab.bsky.social www.nature.com/articles/s41...

PinkyCaMP: an mScarlet-based calcium sensor with enhanced brightness, photostability and multiplexing capabilities - Nature Methods

PinkyCaMP is a red genetically encoded calcium indicator with improved brightness and photostability. Derived from mScarlet, it does not exhibit photoswitching upon blue light illumination and is ther...

nature.com

PinkyCaMP is finally published!! My first paper co-first authored with @sikmys.bsky.social where we make a great optogenetic and multiplex compatible red calcium indicator. Thank you to @massecklab.bsky.social and @campbell-lab.bsky.social for guiding this journey www.nature.com/articles/s41...

PinkyCaMP: an mScarlet-based calcium sensor with enhanced brightness, photostability and multiplexing capabilities - Nature Methods

PinkyCaMP is a red genetically encoded calcium indicator with improved brightness and photostability. Derived from mScarlet, it does not exhibit photoswitching upon blue light illumination and is ther...

nature.com

What if you got to work with some of your best friends on a science project? I can't publish how fun this was, but I can show you the data (🧵)! Last week, we posted our second neutrophil swarming paper to bioRxiv and I wanted to post my favorite videos here~ www.biorxiv.org/content/10.6...

TIL "dynamic range" is used for two orthogonal biosensor properties. Which one is correct?

📣 New preprint alert! Co-authored by @spalaciosm.bsky.social and @andreacaldarola.bsky.social : Quantitative imaging of calcium dynamics with a green fluorescent biosensor and fluorescence lifetime imaging www.biorxiv.org/content/10.6...

Such a beautiful example demonstrating the importance of developing and using robust biosensors #FluorescenceFriday

Tommaso Patriarchi@tpatriarchi.bsky.social · 4mo ago

Wait… localized norepinephrine transients in the awake visual cortex?! Who would have guessed this neuromodulatory signal is that spatially precise, right where visual processing is happening. Brain state control just got a lot more local. @ruedigersarah.bsky.social www.nature.com/articles/s41...

Wait… localized norepinephrine transients in the awake visual cortex?! Who would have guessed this neuromodulatory signal is that spatially precise, right where visual processing is happening. Brain state control just got a lot more local. @ruedigersarah.bsky.social www.nature.com/articles/s41...

Science professors and educators all have a role in combating this eugenics resurgence www.sciencedirect.com/science/arti...

NBC News@nbcnews.com · 5mo ago

President Trump blames "the genetics" of the assailants in a string of recents attacks including an improvised explosive thrown outside Gracie Mansion in New York City and attacks at a university in Virginia and a synagogue in Michigan.

This is why we love #CalciumSignaling Look how mechanical damage triggers long range Ca2+ waves in this plant !! By @annalisabellandi.bsky.social, who is now around here ;) Full www.science.org/doi/10.1126/... @science.org #microscopy #cell #mechanobiology 🧪🔬

The most sensitive green and red #norepinephrine #sensors engineered to date are out in #naturemethods! Deeply grateful to all our funders and contributors who made this work possible. A true team effort. @erc.europa.eu @snsf.ch doi.org/10.1038/s415...

Next-generation multicolor indicators for in vivo imaging of norepinephrine - Nature Methods

Improved red and green indicators for norepinephrine and their characterization are reported. These indicators allow detection of norepinephrine release in awake behaving mice in dual-color fiber phot...

doi.org

Abolish ICE. There’s no reforming it. There’s no compromise. There’s only one way to rein in ICE’s terror campaign. Abolish it.

Great to see this officially out: An mTurquoise2-Based Glucose Biosensor - works in yeast & mammalian cells and is compatible with FLIM: pubs.acs.org/doi/10.1021/...

An mTurquoise2-Based Glucose Biosensor

Glucose is an important substrate for organisms to acquire energy needed for cellular growth. Despite the importance of this metabolite, single-cell information at a fast time-scale about the dynamics...

pubs.acs.org

Today I'd like to honor the memory of my mentor and friend, Roger Tsien, born 1952 February 1. Today would have been Roger's 74th birthday. Most know Roger for his 2008 Chemistry Nobel Prize with Shimomura and Chalfie. Roger made GFP into the versatile imaging method it is now.

An engineered nanobody inhibitor for molecular-to-circuit control of opioid receptor function. This was a really fun collaboration with @miriamstoeber.bsky.social and @amanglik.bsky.social labs, check it out! www.biorxiv.org/content/10.6...

We've got ISSUES. Literally. We scraped >100k special issues & over 1 million articles to bring you a PISS-poor paper. We quantify just how many excess papers are published by guest editors abusing special issues to boost their CVs. How bad is it & what can we do? arxiv.org/abs/2601.07563 A 🧵 1/n

In this mesmerizing video by our #ElectronMicroscopy team, a layer 5 thin tufted neuron is revealed with blue dendrites and purple axon. As it progresses, we zoom into where it connects to a pyramidal cell. Explore our EM data at: microns-explorer.org

The 'Version of Record' of our paper came out on #FluorescenceFriday elifesciences.org/articles/105...

A green lifetime biosensor for calcium that remains bright over its full dynamic range

Directed evolution was used to engineer a green fluorescent biosensor for quantitative calcium imaging that remains fluorescent, yet shows a large change in lifetime in cells.

elifesciences.org

@massecklab.bsky.social @finkryan.bsky.social 🥳👏🧠🐭🔬🧪🩷