🚀 #CryoSPARC v5.1 is now available! With major new features and performance improvements for more powerful, efficient, and accessible #cryo-EM data processing workflows. Explore the highlights 👇
Cooling fast and slow: Characterizing the effects of vitrification in cryo-EM and the subsequent recovery of equilibrium populations pubmed.ncbi.nlm.nih.gov/42975931/ #cryoEM
CryoID2: deep learning-enhanced identification of protein and RNA from cryoEM maps https://www.biorxiv.org/content/10.64898/2026.11.03.764069v1
Happy to be one of the newest micro-crane operators 👷♀️ (cryo-lift out) #teamtomo. We now can explore the frontiers of tissue samples!! We are so grateful to @3p1l.bsky.social @humantechnopole.bsky.social who trained us on the SOLIST method. Please enjoy this skyscraper 🏙️ of a tissue section 🤗
Preserving information and integrity in Cryo-EM: Toward a Fourier-space deposition approach pubmed.ncbi.nlm.nih.gov/42956948/ #cryoEM
Afinity-functionalized and engineered cryo-EM supports are reviewed as a unified class of specimen-preparation technologies and the major validated platforms are compared #CryoElectronMicroscopy #AffinityGrids #SpecimenPreparation doi.org/10.1107/S205...
🔬 Preferred orientation is a familiar challenge in #cryoEM, but can smarter data processing help overcome it? While developing Automated Workflow v2 in #CryoSPARC, we explored how different particle picking and curation strategies could help address this challenge. 👉 cryosparc.com/blog/automat...
Curious how cryo-ET reveals cells in 3D? Join the Harvard Cryo-EM Center & Thermo Fisher Dec 7–8 at HMS for our first cryo-ET workshop: top speakers, methods workshops, posters, a facility tour & pub trivia. Only 80 seats! Register by Dec 1: forms.cloud.microsoft/r/GfTQ7kg9Yx #CryoET
New on the cryoET Data Portal: three cryo-electron tomography datasets benchmarking the dual laser phase plate (xLPP). It imaged PP7 virus-like particles in an E. coli cell, confirming unprecedented resolution. Explore the data: cryoetdataportal.czscience.com/depositions/...
Replacing H2O with D2O in cryo-EM reduces radiation damage in biological samples www.biorxiv.org/content/10.64898/2026.10.24.761413v1 #cryoEM
Rapid femtosecond laser trenching accelerates cryo-electron tomography lamella workflows www.biorxiv.org/content/10.64898/2026.10.23.761368v1 #cryoEM
interesting details here on the data processing used to get the small particle to high resolution, including the use of HR-HAIR
cryoEM papers@cryoempapers.bsky.social · 3w agoCryo-EM structure of the sub-50 kD PDZ-RhoGEF catalytic core-RhoA complex reveals an allosteric mechanism for GEF switch www.biorxiv.org/content/10.64898/2026.10.21.760610v1 #cryoEM
Fluorescent Biosensors for Cryogenic Correlative Light and Electron Microscopy www.biorxiv.org/content/10.64898/2026.10.15.759416v1 #cryoEM
This Nov and Dec, we’re hosting Innovation Forums on cryo-ET in Eindhoven and vEM in Brno, bringing together small groups for a look inside the working factory, live demonstrations, and discussion of the challenges shaping research workflows. Interested? Message me here or on email for more details.
Struggling to choose a pixel size for your cryo-ET acquisition? Worry not: super-resolution processing can take in situ subtomogram averaging beyond physical Nyquist! We reached 2.8 A loc-res of a ribosome dataset with 4.78 A physical Nyquist limit! Our new preprint 👇: doi.org/10.64898/202...
Exciting times for cryo-EM in NL 🇳🇱! As part of EMPower, two new Titan Krios G5 microscopes have been installed at NeCEN in Leiden and Utrecht. First benchmarks with ApoF reached 1.12 Å resolution 🤯. Looking forward to seeing all the exciting science these microscopes will enable! 🔬
Community Announcements: Upcoming events from our partners at @instruct-eric.bsky.social. Scipion for Facilities Workshop Oct 29-30, 2026| Madrid, Spain Organized in collaboration with @cnb-csic.bsky.social (CNB) Register here: buff.ly/Xr6cgsb #SBGrid #Collab
Scipion for facilities Workshop. October 29 - 30, 2026
Cryo-EM Facilities Meeting 2026 Workshop Information Meeting Information Venue Hybrid Location National Center for Biotechnology (CNB), Madrid, Spain Date October 29–30, 2026 Contact I2PC – Instruct…
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Variable-resolution maps (VRMs) in CCTBX and Phenix: accounting for local resolution in cryoEM pubmed.ncbi.nlm.nih.gov/42878395/ #cryoEM
Workflow and instrumentation for multiscale correlative imaging in voluminous cryogenic specimens www.biorxiv.org/content/10.64898/2026.10.14.759080v1 #cryoEM
Single-particle cryoEM: Theory and practice pubmed.ncbi.nlm.nih.gov/42876405/ #cryoEM
Shawn Zheng leads Biohub’s work to streamline cryo-electron tomography, which images cells in 3D at near-atomic resolution. His team’s AreTomoLive automates cryoET reconstruction and denoising in real time and cuts processing from months to days. Learn more: sbgrid.org/software/tal...
New on the #cryoET Data Portal: three datasets benchmarking the crossed laser phase plate (xLPP) ⚡ Data by Reza Paraan and the @biohub.org team with collaborators at UC Berkeley. Full credits at the end of the 🧵 cryoetdataportal.czscience.com/depositions/10363
🎥 Recordings from the recent Wellcome-MRC In Situ Cryo-ET Course 2026 are now available on YouTube! From sample preparation and cryo-FIB milling to data collection, image processing and STA, check it out here: www.youtube.com/playlist?lis... #CryoET #CryoEM #StructuralBiology #eBIC #Microscopy
Wellcome-MRC in situ Cryo-ET Workshop 2026 - YouTube
This lecture series covers the fundamental principles and workflows of cryo-electron tomography (cryo-ET), including sample preparation, focused ion beam (FI...
youtube.com
Ab initio CryoEM maps minimizing preferred orientation problems in heterogeneous mixtures: ReconSIREN www.biorxiv.org/content/10.64898/2026.10.03.756391v1 #cryoEM
Interested in the theory and practice of #CLEM? Sign up now by 25 Oct for the EMBO Practical Course "In situ CLEM at room temperature and in cryo" in Heidelberg, DE, 31 Jan–5 Feb 2027. https://www.embl.org/about/info/course-and-conference-office/events/lem27-01/ #EMBOCLEM #EMBOevents #training 🧪
Our latest development is now on bioRxiv: cryoSHIELDs — amphipathic peptide additives that make cryo-EM grid prep far more reliable. Tested across 24 protein systems, they enabled the first ABCC6 structure and GPCR–G protein structures at just 0.1–0.5 mg/mL! www.biorxiv.org/content/10.6...
Graphene reservoirs formed over perforated support films precisely control ice thickness during cryo-EM sample preparation. www.nature.com/articles/s41...
Control of ice thickness in cryo-EM via confinement - Nature Methods
This study explores the use of graphene reservoirs formed over perforated supporting films as a method to precisely control ice thickness during cryo-EM sample preparation.
nature.com
Plunge freezing tweezers with ultrasonic excitation for reducing preferred particle orientation in cryo-EM https://www.biorxiv.org/content/10.64898/2026.09.29.755300v1